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J. Biol. Chem., Vol. 265, Issue 24, 14298-14307, Aug, 1990

Import of chemically synthesized signal peptides into rat liver mitochondria

YK Pak and H Weiner
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.

The signal peptides of pre-aldehyde dehydrogenase (22-mer) and pre- ornithine transcarbamylase (27-mer) were chemically synthesized and their imports into rat liver mitochondria were studied. Both signal peptides were imported rapidly (within 2 min) in the absence of a membrane potential, exogenous ATP, or rabbit reticulocyte lysate. Signal peptides also were imported into mitochondria treated with a low concentration of trypsin which removed the outer membrane proteins. It was concluded that the chemically synthesized signal peptide could be imported differently than the precursor proteins. The imported signal peptide were found to be associated with both outer and inner membranes. Pulse-chase experiments showed that the import was unidirectional and that the signal peptides associated with inner membranes increased during the chase time. The signal peptides inhibited import of precursor proteins to different extents. Association of signal peptides with inner membrane near or at translocator sites might result in inhibition of precursor import.
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