HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Horowitz, B. Articles by McDonough, A. A. Search for Related Content PubMed PubMed Citation Articles by Horowitz, B. Articles by McDonough, A. A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 24, 14308-14314, 08, 1990

Differential regulation of Na,K-ATPase alpha 1, alpha 2, and beta subunit mRNA and protein levels by thyroid hormone

B Horowitz, CB Hensley, M Quintero, KK Azuma, D Putnam and AA McDonough
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.

The purpose of this study was to determine the effect of thyroid status on the Na,K-ATPase alpha isoforms and beta in rat heart, skeletal muscle, kidney, and brain at the levels of mRNA, protein abundance, and enzymatic activity. Northern and dot-blot analysis of RNA (euthyroid, hypothyroid, and triiodothyronine-injected hypothyroids = hyperthyroids) and immunoblot analysis of protein (euthyroid and hypothyroid) revealed isoform-specific regulation of Na,K-ATPase by thyroid status in kidney, heart, and skeletal muscle and no regulation of sodium pump subunit levels in the brain. In general, in the transition from euthyroid to hypothyroid alpha 1 mRNA and protein levels are unchanged in kidney and skeletal muscle and slightly decreased in heart, while alpha 2 mRNA and protein are decreased significantly in heart and skeletal muscle. In hypothyroid heart and skeletal muscle, the decrease in alpha 2 protein levels was much greater than the decrease in alpha 2 mRNA levels relative to euthyroid indicating translational or post-translational regulation of alpha 2 protein abundance by triiodothyronine status in these tissues. The regulation of beta subunit by thyroid status is tissue-dependent. In hypothyroid kidney beta mRNA levels do not change, but immunodetectable beta protein levels decrease relative to euthyroid, and the decrease parallels the decrease in Na,K-ATPase activity. In hypothyroid heart and skeletal muscle beta mRNA levels decrease; beta protein decreases in heart and was not detected in the skeletal muscle. These findings demonstrate that the euthyroid levels of expression of alpha 1 in heart, alpha 2 in heart and skeletal muscle, and beta in kidney, heart, and skeletal muscle are dependent on the presence of thyroid hormone.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Bhargava, M. R. Runyon, D. Smirnov, J. Lei, T. J. Groppoli, C. N. Mariash, O. D. Wangensteen, and D. H. Ingbar Triiodo-L-thyronine Rapidly Stimulates Alveolar Fluid Clearance in Normal and Hyperoxia-injured Lungs Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 506 - 512. [Abstract] [Full Text] [PDF]

				C. Garcia-G, L. Lopez-Bojorquez, J. Nunez, C. Valverde-R, and A. Orozco 3,5-Diiodothyronine in vivo maintains euthyroidal expression of type 2 iodothyronine deiodinase, growth hormone, and thyroid hormone receptor beta1 in the killifish Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R877 - R883. [Abstract] [Full Text] [PDF]

				B. Phakdeekitcharoen, S. Phudhichareonrat, C. Pookarnjanamorakot, C. Kijkunasathian, N. Tubtong, W. Kittikanokrat, and P. Radinahamed Thyroid Hormone Increases mRNA and Protein Expression of Na+-K+-ATPase {alpha}2 and {beta}1 Subunits in Human Skeletal Muscles J. Clin. Endocrinol. Metab., January 1, 2007; 92(1): 353 - 358. [Abstract] [Full Text] [PDF]

				Y.-C. Chen, M. A. Cadnapaphornchai, J. Yang, S. N. Summer, S. Falk, C. Li, W. Wang, and R. W. Schrier Nonosmotic release of vasopressin and renal aquaporins in impaired urinary dilution in hypothyroidism Am J Physiol Renal Physiol, October 1, 2005; 289(4): F672 - F678. [Abstract] [Full Text] [PDF]

				T. CLAUSEN Na+-K+ Pump Regulation and Skeletal Muscle Contractility Physiol Rev, October 1, 2003; 83(4): 1269 - 1324. [Abstract] [Full Text] [PDF]

				N. Nordsborg, J. Bangsbo, and H. Pilegaard Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism J Appl Physiol, September 1, 2003; 95(3): 1201 - 1206. [Abstract] [Full Text] [PDF]

				J. Lei, S. Nowbar, C. N. Mariash, and D. H. Ingbar Thyroid hormone stimulates Na-K-ATPase activity and its plasma membrane insertion in rat alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L762 - L772. [Abstract] [Full Text] [PDF]

				R. Schmitt, E. Klussmann, T. Kahl, D. H. Ellison, and S. Bachmann Renal expression of sodium transporters and aquaporin-2 in hypothyroid rats Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1097 - F1104. [Abstract] [Full Text] [PDF]

				A. D Pitt, C. Fernandes, N. L Bewick, P. D Hemsworth, K. A Buhagiar, P. S Hansen, H. H Rasmussen, L. Delbridge, and D. W Whalley Chronic amiodarone-induced inhibition of the Na+-K+ pump in rabbit cardiac myocytes is thyroid-dependent: comparison with dronedarone Cardiovasc Res, January 1, 2003; 57(1): 101 - 108. [Abstract] [Full Text] [PDF]

				P. Devarajan and E. J. Benz Jr. Translational regulation of Na-K-ATPase subunit mRNAs by glucocorticoids Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1132 - F1138. [Abstract] [Full Text] [PDF]

				C. B. Thompson, C. Choi, J. H. Youn, and A. A. McDonough Temporal responses of oxidative vs. glycolytic skeletal muscles to K+ deprivation: Na+ pumps and cell cations Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1411 - C1419. [Abstract] [Full Text] [PDF]

				D. F. Gray, A. S. Mihailidou, P. S. Hansen, K. A. Buhagiar, N. L. Bewick, H. H. Rasmussen, and D. W. Whalley Amiodarone Inhibits the Na+-K+ Pump in Rabbit Cardiac Myocytes after Acute and Chronic Treatment J. Pharmacol. Exp. Ther., January 1, 1998; 284(1): 75 - 82. [Abstract] [Full Text]

				Z. Borok, S. I. Danto, L. L. Dimen, X.-L. Zhang, and R. L. Lubman Na+-K+-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF Am J Physiol Lung Cell Mol Physiol, January 1, 1998; 274(1): L149 - L158. [Abstract] [Full Text] [PDF]

				V. Rybin and S. F. Steinberg Thyroid Hormone Represses Protein Kinase C Isoform Expression and Activity in Rat Cardiac Myocytes Circ. Res., September 1, 1996; 79(3): 388 - 398. [Abstract] [Full Text]

				E. Songu-Mize, X. Liu, J. E. Stones, and L. J. Hymel Regulation of Na+,K+-ATPase {alpha}-Subunit Expression by Mechanical Strain in Aortic Smooth Muscle Cells Hypertension, March 1, 1996; 27(3): 827 - 832. [Abstract] [Full Text]