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J. Biol. Chem., Vol. 265, Issue 25, 15294-15299, Sep, 1990
MS Lan, SK Batra, WN Qi, RS Metzgar and MA Hollingsworth
A monospecific polyclonal antiserum against deglycosylated human pancreatic
tumor mucin was used to select human pancreatic mucin cDNA clones from a
lambda gt11 cDNA expression library developed from a human pancreatic tumor
cell line. The full-length 4.4-kilobase mucin cDNA sequence included a
72-base pair 5'-untranslated region and a 307- base pair 3'-untranslated
region. The predicted amino acid sequence for this cDNA revealed a protein
of 122,071 daltons containing 1,255 amino acid residues of which greater
than 60% were serine, threonine, proline, alanine, and glycine.
Approximately two-thirds of the protein sequence consisted of identical
20-amino acid tandem repeats which were flanked by degenerate tandem
repeats and nontandem repeat sequences on both the amino-terminal and
carboxyl-terminal ends. The amino acid sequence also contained five
putative N-linked glycosylation sites, a putative signal sequence and
transmembrane domain, and numerous serine and threonine residues (potential
O-linked glycosylation sites) outside and within the tandem repeat
position. The cDNA and deduced amino acid sequence of the pancreatic mucin
sequence was over 99% homologous with a mucin cDNA sequence derived from
breast tumor mucin, even though the native forms of these molecules are
quite distinct in size and degree of glycosylation.
Cloning and sequencing of a human pancreatic tumor mucin cDNA
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
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H. H. Wandall, H. Hassan, E. Mirgorodskaya, A. K. Kristensen, P. Roepstorff, E. P. Bennett, P. A. Nielsen, M. A. Hollingsworth, J. Burchell, J. Taylor-Papadimitriou, et al. Substrate Specificities of Three Members of the Human UDP-N-Acetyl-alpha -D-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase Family, GalNAc-T1, -T2, and -T3 J. Biol. Chem., September 19, 1997; 272(38): 23503 - 23514. [Abstract] [Full Text] [PDF]
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J.-L. Desseyn, J.-P. Aubert, I. Van Seuningen, N. Porchet, and A. Laine Genomic Organization of the 3' Region of the Human Mucin Gene MUC5B J. Biol. Chem., July 4, 1997; 272(27): 16873 - 16883. [Abstract] [Full Text] [PDF]
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N. Harada, S. Iijima, K. Kobayashi, T. Yoshida, W. R. Brown, T. Hibi, A. Oshima, and M. Morikawa Human IgGFc Binding Protein (Fcgamma BP) in Colonic Epithelial Cells Exhibits Mucin-like Structure J. Biol. Chem., June 13, 1997; 272(24): 15232 - 15241. [Abstract] [Full Text] [PDF]
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L. F. Pemberton, A. Rughetti, J. Taylor-Papadimitriou, and S. J. Gendler The Epithelial Mucin MUC1 Contains at Least Two Discrete Signals Specifying Membrane Localization in Cells J. Biol. Chem., January 26, 1996; 271(4): 2332 - 2340. [Abstract] [Full Text] [PDF]
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A. P. Spicer, G. J. Rowse, T. K. Lidner, and S. J. Gendler Delayed Mammary Tumor Progression in Muc-1 Null Mice J. Biol. Chem., December 15, 1995; 270(50): 30093 - 30101. [Abstract] [Full Text] [PDF]
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T.éc. Lesuffleur, F. Roche, A. S. Hill, M. Lacasa, M. Fox, D. M. Swallow, A. Zweibaum, and F. X. Real Characterization of a Mucin cDNA Clone Isolated from HT-29 Mucus-secreting Cells J. Biol. Chem., June 9, 1995; 270(23): 13665 - 13673. [Abstract] [Full Text] [PDF]
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T Lesuffleur, N Porchet, J. Aubert, D Swallow, J. Gum, Y. Kim, F. Real, and A Zweibaum Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT-29 cell subpopulations J. Cell Sci., January 11, 1993; 106(3): 771 - 783. [Abstract] [PDF]
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V. Braga and S. Gendler Modulation of Muc-1 mucin expression in the mouse uterus during the estrus cycle, early pregnancy and placentation J. Cell Sci., January 6, 1993; 105(2): 397 - 405. [Abstract] [PDF]
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J. Chambers and A Harris Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells J. Cell Sci., January 6, 1993; 105(2): 417 - 422. [Abstract] [PDF]
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 R. Brandenberger, A. Schmidt, J. Linton, D. Wang, C. Backus, S. Denda, U. Muller, and L. F. Reichardt Identification and characterization of a novel extracellular matrix protein nephronectin that is associated with integrin {alpha}8{beta}1 in the embryonic kidney J. Cell Biol., July 23, 2001; 154(2): 447 - 458. [Abstract] [Full Text] [PDF]
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A. Choudhury, R. K. Singh, N. Moniaux, T. H. El-Metwally, J.-P. Aubert, and S. K. Batra Retinoic Acid-dependent Transforming Growth Factor-beta 2-mediated Induction of MUC4 Mucin Expression in Human Pancreatic Tumor Cells Follows Retinoic Acid Receptor-alpha Signaling Pathway J. Biol. Chem., October 20, 2000; 275(43): 33929 - 33936. [Abstract] [Full Text] [PDF]
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K. Engelmann, S. E. Baldus, and F.-G. Hanisch Identification and Topology of Variant Sequences within Individual Repeat Domains of the Human Epithelial Tumor Mucin MUC1 J. Biol. Chem., July 20, 2001; 276(30): 27764 - 27769. [Abstract] [Full Text] [PDF]
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E. P. Lillehoj, B. T. Kim, and K. C. Kim Identification of Pseudomonas aeruginosa flagellin as an adhesin for Muc1 mucin Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L751 - L756. [Abstract] [Full Text] [PDF]
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