HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allore, R. J. Articles by Marks, A. Search for Related Content PubMed PubMed Citation Articles by Allore, R. J. Articles by Marks, A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 26, 15537-15543, Sep, 1990

Cloning and expression of the human S100 beta gene

RJ Allore, WC Friend, D O'Hanlon, KM Neilson, R Baumal, RJ Dunn and A Marks
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

S100 protein is a low molecular weight, EF-hand, Ca2(+)-binding protein widely distributed and conserved in the central nervous system of vertebrates. The gene coding for the beta subunit of human S100 protein (S100 beta) has been recently mapped to chromosome 21. In order to study the expression of this gene in normal and abnormal brain development, we have isolated and characterized overlapping genomic clones spanning the region coding for human S100 beta and its flanking sequences. The intron-exon organization of the human S100 beta gene is similar to that of the genes coding for several other members of the S100 protein subfamily of EF-hand proteins. The human S100 beta gene is composed of 3 exons, the first of which specifies the 5'-untranslated region, while the second and third each encode a single EF-hand, Ca2(+)- binding domain. The promoter region contains several potential regulatory transcription elements including the cAMP-responsive elements CRE and AP-2. A novel sequence motif, the S100 protein element, situated in close proximity to the TATA box of the genes of several members of the S100 protein subfamily, has been identified. In addition, multiple repeats with similar nucleotide sequence and location to the recently reported beta globin direct repeat elements have been also found in the human S100 beta promoter. A full length (17.3 kilobases) copy of the human S100 beta gene was constructed and transfected into rat glioma C6 cells. Stable transfectants were shown to express correctly initiated transcripts of the human S100 beta gene, indicating that the cloned sequences contain functional regulatory transcription elements.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Zuo, J. L. Lubischer, H. Kang, L. Tian, M. Mikesh, A. Marks, V. L. Scofield, S. Maika, C. Newman, P. Krieg, et al. Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination J. Neurosci., December 8, 2004; 24(49): 10999 - 11009. [Abstract] [Full Text] [PDF]

				M. P. Crawford, S. X. Yan, S. B. Ortega, R. S. Mehta, R. E. Hewitt, D. A. Price, P. Stastny, D. C. Douek, R. A. Koup, M. K. Racke, et al. High prevalence of autoreactive, neuroantigen-specific CD8+ T cells in multiple sclerosis revealed by novel flow cytometric assay Blood, June 1, 2004; 103(11): 4222 - 4231. [Abstract] [Full Text] [PDF]

				J. N. Tsoporis, A. Marks, L. J. Van Eldik, D. O'Hanlon, and T. G. Parker Regulation of the S100B gene by alpha 1-adrenergic stimulation in cardiac myocytes Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H193 - H203. [Abstract] [Full Text] [PDF]

				J. N. Tsoporis, A. Marks, H. J. Kahn, J. W. Butany, P. P. Liu, D. O'Hanlon, and T. G. Parker S100beta Inhibits alpha 1-Adrenergic Induction of the Hypertrophic Phenotype in Cardiac Myocytes J. Biol. Chem., December 12, 1997; 272(50): 31915 - 31921. [Abstract] [Full Text] [PDF]

				D. B. Zimmer, J. Chessher, G. L. Wilson, and W. E. Zimmer S100A1 and S100B Expression and Target Proteins in Type I Diabetes Endocrinology, December 1, 1997; 138(12): 5176 - 5183. [Abstract] [Full Text] [PDF]