| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 3, 1524-1530, 01, 1990
RD Hanson, NL Connolly, D Burnett, EJ Campbell, RM Senior and TJ Ley
Cathepsin G is a neutral serine protease that is found in the azurophil
granules of neutrophils and monocytes. Previous experiments had
demonstrated that cathepsin G is actively produced by the promonocytic U937
cell line, and that 12-O-tetradecanoylphorbol-13-acetate (TPA)- induced
differentiation of these cells toward macrophages resulted in a reduction
of cathepsin G activity. In this study, we have analyzed the mechanism of
this TPA-induced down-regulatory event. Using in situ hybridization
techniques, we demonstrated that cathepsin G mRNA is detectable only at the
promyelocytic stage of myeloid development. Using U937 promonocytic cells
as a model, we demonstrated; 1) cathepsin G protein levels decline in
TPA-treated cells; 2) this decline was due to a nearly complete loss of
cathepsin G mRNA in cells treated with TPA for 24 h; and 3) the rate of
cathepsin G mRNA loss with TPA treatment was similar to that with
actinomycin D. These results suggested that cathepsin G transcription was
down-regulated within several hours of TPA addition. This was directly
tested by performing nuclear run-off assays of TPA-treated U937 cells;
cathepsin G transcription was shown to be strand-specific, and declined
within 4 h of TPA addition. Cathepsin G transcription was essentially
undetectable 8 or more hours after TPA treatment, suggesting that
down-regulation is predominantly transcriptional. Cycloheximide treatment
of U937 cells resulted in a partial block of TPA-mediated cathepsin G
down-regulation, indicating that continuous protein synthesis is required
for down-regulation to occur. A newly synthesized protein or proteins may
therefore be required for the transcriptional down-regulation of cathepsin
G during the normal development of promyelocytes or promonocytes.
Developmental regulation of the human cathepsin G gene in myelomonocytic cells
Department of Medicine Jewish Hospital, Washington University Medical Center, St. Louis, Missouri 63110.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. Akk, P. M. Simmons, H. W. Chan, E. Agapov, M. J. Holtzman, M. H. Grayson, and C. T. N. Pham Dipeptidyl Peptidase I-Dependent Neutrophil Recruitment Modulates the Inflammatory Response to Sendai Virus Infection J. Immunol., March 1, 2008; 180(5): 3535 - 3542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, P. Haviernik, K. D. Bunting, and Y.-C. Yang Cited2 is required for normal hematopoiesis in the murine fetal liver Blood, October 15, 2007; 110(8): 2889 - 2898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Stewart, H. N. Rivera, R. Karls, F. D. Quinn, J. Roman, and C. A. Rivera-Marrero Increased pathology in lungs of mice after infection with an {alpha}-crystallin mutant of Mycobacterium tuberculosis: changes in cathepsin proteases and certain cytokines Microbiology, January 1, 2006; 152(1): 233 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. de Garavilla, M. N. Greco, N. Sukumar, Z.-W. Chen, A. O. Pineda, F. S. Mathews, E. Di Cera, E. C. Giardino, G. I. Wells, B. J. Haertlein, et al. A Novel, Potent Dual Inhibitor of the Leukocyte Proteases Cathepsin G and Chymase: MOLECULAR MECHANISMS AND ANTI-INFLAMMATORY ACTIVITY IN VIVO J. Biol. Chem., May 6, 2005; 280(18): 18001 - 18007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Rivera-Marrero, J. Stewart, W. M. Shafer, and J. Roman The Down-Regulation of Cathepsin G in THP-1 Monocytes after Infection with Mycobacterium tuberculosis Is Associated with Increased Intracellular Survival of Bacilli Infect. Immun., October 1, 2004; 72(10): 5712 - 5721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Sedelies, T. J. Sayers, K. M. Edwards, W. Chen, D. G. Pellicci, D. I. Godfrey, and J. A. Trapani Discordant Regulation of Granzyme H and Granzyme B Expression in Human Lymphocytes J. Biol. Chem., June 18, 2004; 279(25): 26581 - 26587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Westervelt, A. A. Lane, J. L. Pollock, K. Oldfather, M. S. Holt, D. B. Zimonjic, N. C. Popescu, J. F. DiPersio, and T. J. Ley High-penetrance mouse model of acute promyelocytic leukemia with very low levels of PML-RAR{alpha} expression Blood, September 1, 2003; 102(5): 1857 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Frazar, J. L. Weisbein, S. M. Anderson, A. P. Cline, L. J. Garrett, G. Felsenfeld, P. G. Gallagher, and D. M. Bodine Variegated Expression from the Murine Band 3 (AE1) Promoter in Transgenic Mice Is Associated with mRNA Transcript Initiation at Upstream Start Sites and Can Be Suppressed by the Addition of the Chicken {beta}-Globin 5' HS4 Insulator Element Mol. Cell. Biol., July 15, 2003; 23(14): 4753 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-M. Herrmann, H. Funke-Kaiser, K. Schmidt-Petersen, V. Nicaud, M. Gautier-Bertrand, A. Evans, F. Kee, D. Arveiler, C. Morrison, H.-D. Orzechowski, et al. Characterization of Polymorphic Structure of Cathepsin G Gene: Role in Cardiovascular and Cerebrovascular Diseases Arterioscler. Thromb. Vasc. Biol., September 1, 2001; 21(9): 1538 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ferrari-Lacraz, L. P. Nicod, R. Chicheportiche, H. G. Welgus, and J.-M. Dayer Human Lung Tissue Macrophages, but not Alveolar Macrophages, Express Matrix Metalloproteinases after Direct Contact with Activated T Lymphocytes Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 442 - 451. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. BUREAU, G. BONIZZI, N. KIRSCHVINK, S. DELHALLE, D. DESMECHT, M.-P. MERVILLE, V. BOURS, and P. LEKEUX Correlation between Nuclear Factor-kappa B Activity in Bronchial Brushing Samples and Lung Dysfunction in an Animal Model of Asthma Am. J. Respir. Crit. Care Med., April 1, 2000; 161(4): 1314 - 1321. [Abstract] [Full Text]
|
|
|
|
|
|
D. M. MacIvor, S. D. Shapiro, C. T.N. Pham, A. Belaaouaj, S. N. Abraham, and T. J. Ley Normal Neutrophil Function in Cathepsin G-Deficient Mice Blood, December 15, 1999; 94(12): 4282 - 4293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Melnick and J. D. Licht Deconstructing a Disease: RAR{alpha}, Its Fusion Partners, and Their Roles in the Pathogenesis of Acute Promyelocytic Leukemia Blood, May 15, 1999; 93(10): 3167 - 3215. [Full Text] [PDF]
|
|
|
|
|
|
J. J. Baldassare, Y. Bi, and C. J. Bellone The Role of p38 Mitogen-Activated Protein Kinase in IL-1{beta} Transcription J. Immunol., May 1, 1999; 162(9): 5367 - 5373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Rees, R. A. Rogers, J. Cooley, R. J. Mandle, D. M. Kenney, and E. Remold-O'Donnell Recombinant Human Monocyte/Neutrophil Elastase Inhibitor Protects Rat Lungs against Injury from Cystic Fibrosis Airway Secretions Am. J. Respir. Cell Mol. Biol., January 1, 1999; 20(1): 69 - 78. [Abstract] [Full Text]
|
|
|
|
|
|
D. Garwicz, A. Lindmark, A.-M. Persson, and U. Gullberg On the Role of the Proform-Conformation for Processing and Intracellular Sorting of Human Cathepsin G Blood, August 15, 1998; 92(4): 1415 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Makar, C. T. N. Pham, M. H. Dehoff, S. M. O'Connor, S. M. Jacobi, and V. M. Holers An Intronic Silencer Regulates B Lymphocyte Cell- and Stage-Specific Expression of the Human Complement Receptor Type 2 (CR2, CD21) Gene J. Immunol., February 1, 1998; 160(3): 1268 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. V. Rao, G. V. Rao, and J. R. Hoidal Human Dipeptidyl-peptidase I. GENE CHARACTERIZATION, LOCALIZATION, AND EXPRESSION J. Biol. Chem., April 11, 1997; 272(15): 10260 - 10265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Grisolano, R. L. Wesselschmidt, P. G. Pelicci, and T. J. Ley Altered Myeloid Development and Acute Leukemia in Transgenic Mice Expressing PML-RARalpha Under Control of Cathepsin G Regulatory Sequences Blood, January 15, 1997; 89(2): 376 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sturrock, K. F. Franklin, and J. R. Hoidal Human Proteinase-3 Expression Is Regulated by PU.1 in Conjunction with a Cytidine-rich Element J. Biol. Chem., December 13, 1996; 271(50): 32392 - 32402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. N. Pham, D. M. MacIvor, B. A. Hug, J. W. Heusel, and T. J. Ley Long-range disruption of gene expression by a selectable marker cassette PNAS, November 12, 1996; 93(23): 13090 - 13095. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
