| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 31, 18867-18870, Nov, 1990
K Boesze-Battaglia, SJ Fliesler and AD Albert
The initial events of visual transduction occur on disc membranes which are
sequestered within the photoreceptor outer segment. In rod cells, the discs
are stacked in the outer segment. Discs are formed at the base of the rod
outer segment (ROS) from evaginations of the plasma membrane. As new discs
form, older discs move toward the apical tip of the rod, from which they
are eventually shed and subsequently phagocytosed by the adjacent pigment
epithelium. Thus, disc membranes within a given rod cell are not of uniform
age. We have recently shown that disc membranes are not homogeneous with
respect to cholesterol content (Boesze-Battaglia, K., Hennessey, T., and
Albert, A. D. (1989) J. Biol. Chem. 264, 8151-8155). In the present study,
freshly isolated bovine retinas were incubated with [3H]leucine for 4 h in
order to allow sufficient time for the radiolabeled proteins to become
incorporated into the basal-most (newest) discs. Osmotically intact discs
were then isolated. After the addition of digitonin, the discs were
fractionated based on cholesterol content, and radioactivity (indicative of
newly synthesized protein) was measured. Discs which exhibited high
cholesterol content also exhibited high radio-activity. These results
demonstrate that the cholesterol heterogeneity of ROS disc membranes is
related to the age, and thus the position, of the discs in the ROS.
Relationship of cholesterol content to spatial distribution and age of disc membranes in retinal rod outer segments
Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York, Buffalo 14214.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Bretillon, N. Acar, M. W. Seeliger, M. Santos, M. A. Maire, P. Juaneda, L. Martine, S. Gregoire, C. Joffre, A. M. Bron, et al. ApoB100,LDLR-/- Mice Exhibit Reduced Electroretinographic Response and Cholesteryl Esters Deposits in the Retina Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1307 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-I. Yim, S. Scarselletta, F. Zang, X. Wu, D.-w. Lee, Y.-s. Kang, E. Eisenberg, and L. E. Greene Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells J. Cell Sci., June 1, 2005; 118(11): 2405 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Martin, M. H. Elliott, R. S. Brush, and R. E. Anderson Detailed Characterization of the Lipid Composition of Detergent-Resistant Membranes from Photoreceptor Rod Outer Segment Membranes Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1147 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Huster, H. A. Scheidt, K. Arnold, A. Herrmann, and P. Muller Desmosterol May Replace Cholesterol in Lipid Membranes Biophys. J., March 1, 2005; 88(3): 1838 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. I. Senin, D. Hoppner-Heitmann, O. O. Polkovnikova, V. A. Churumova, N. K. Tikhomirova, P. P. Philippov, and K.-W. Koch Recoverin and Rhodopsin Kinase Activity in Detergent-resistant Membrane Rafts from Rod Outer Segments J. Biol. Chem., November 19, 2004; 279(47): 48647 - 48653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Boesze-Battaglia, J. Dispoto, and M. A. Kahoe Association of a Photoreceptor-specific Tetraspanin Protein, ROM-1, with Triton X-100-resistant Membrane Rafts from Rod Outer Segment Disk Membranes J. Biol. Chem., October 25, 2002; 277(44): 41843 - 41849. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
