| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 35, 21411-21414, 12, 1990
YB Xie, H Wang and DL Segaloff
The lutropin-choriogonadotropin (LH/CG) receptor is a cell surface receptor
comprised of two domains of roughly equivalent size. The amino- terminal
half of the receptor is relatively hydrophilic and is located
extracellularly, whereas the carboxyl-terminal half of the receptor shares
amino acid homology with other receptors that couple to G proteins and is
similarly thought to span the plasma membrane seven times, ending with a
relatively short carboxyl-terminal tail. In order to test the role of the
extracellular domain in binding hormone, we constructed a mutated rat
luteal LH/CG receptor cDNA (termed pCLHR-D2), which encodes for only the
extracellular domain, and used it to transiently transfect human kidney 293
cells. Here we report that the expressed extracellular domain of the LH/CG
receptor is capable of binding human CG with a high affinity, comparable
with that of the full- length receptor. Thus, not only is the extracellular
domain of the glycoprotein hormone receptors involved in binding hormone,
but it alone is capable of conferring high affinity binding. Unexpectedly,
it was also found that this truncated receptor is not secreted into the
culture media but remains trapped within the cells.
Extracellular domain of lutropin/choriogonadotropin receptor expressed in transfected cells binds choriogonadotropin with high affinity
Population Council, New York, New York 10021.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Nurwakagari, A. Breit, C. Hess, H. Salman-Livny, D. Ben-Menahem, and T. Gudermann A conformational contribution of the luteinizing hormone-receptor ectodomain to receptor activation J. Mol. Endocrinol., February 1, 2007; 38(2): 259 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y.-K. Leung, P. J. Steinbach, D. Bear, V. Baxendale, P. Y. Fechner, O. M. Rennert, and W.-Y. Chan Biological Effect of a Novel Mutation in the Third Leucine-Rich Repeat of Human Luteinizing Hormone Receptor Mol. Endocrinol., October 1, 2006; 20(10): 2493 - 2503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Apaja, J. T. Tuusa, E. M. Pietila, H. J. Rajaniemi, and U. E. Petaja-Repo Luteinizing Hormone Receptor Ectodomain Splice Variant Misroutes the Full-Length Receptor into a Subcompartment of the Endoplasmic Reticulum Mol. Biol. Cell, May 1, 2006; 17(5): 2243 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Hawtin, V. J. Wesley, J. Simms, C. C. H. Argent, K. Latif, and M. Wheatley The N-Terminal Juxtamembrane Segment of the V1a Vasopressin Receptor Provides Two Independent Epitopes Required for High-Affinity Agonist Binding and Signaling Mol. Endocrinol., November 1, 2005; 19(11): 2871 - 2881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mesnier and J.-L. Baneres Cooperative Conformational Changes in a G-protein-coupled Receptor Dimer, the Leukotriene B4 Receptor BLT1 J. Biol. Chem., November 26, 2004; 279(48): 49664 - 49670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Chen, G. Rogge, C. Hague, D. Alewood, B. Colless, R. J. Lewis, and K. P. Minneman Subtype-selective Noncompetitive or Competitive Inhibition of Human {alpha}1-Adrenergic Receptors by {rho}-TIA J. Biol. Chem., August 20, 2004; 279(34): 35326 - 35333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Madhra, E. Gay, H. M. Fraser, and W.C. Duncan Alternative splicing of the human luteal LH receptor during luteolysis and maternal recognition of pregnancy Mol. Hum. Reprod., August 1, 2004; 10(8): 599 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fanelli, M. Verhoef-Post, M. Timmerman, A. Zeilemaker, J. W. M. Martens, and A. P. N. Themmen Insight into Mutation-Induced Activation of the Luteinizing Hormone Receptor: Molecular Simulations Predict the Functional Behavior of Engineered Mutants at M398 Mol. Endocrinol., June 1, 2004; 18(6): 1499 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sohn, H. Youn, M. Jeoung, Y. Koo, C. Yi, I. Ji, and T. H. Ji Orientation of Follicle-stimulating Hormone (FSH) Subunits Complexed with the FSH Receptor: {beta} SUBUNIT TOWARD THE N TERMINUS OF EXODOMAIN AND {alpha} SUBUNIT TO EXOLOOP 3 J. Biol. Chem., November 28, 2003; 278(48): 47868 - 47876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Funaro, A. Sapino, B. Ferranti, A. L. Horenstein, I. Castellano, B. Bagni, G. Garotta, and F. Malavasi Functional, Structural, and Distribution Analysis of the Chorionic Gonadotropin Receptor Using Murine Monoclonal Antibodies J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5537 - 5546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Saint-Dizier, M. Chopineau, J. Dupont, P. F. Daels, and Y. Combarnous Expression and Binding Activity of Luteinizing Hormone/Chorionic Gonadotropin Receptors in the Primary Corpus Luteum During Early Pregnancy in the Mare Biol Reprod, November 1, 2003; 69(5): 1743 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Fralish, B. Dattilo, and D. Puett Structural Analysis of Yoked Chorionic Gonadotropin-Luteinizing Hormone Receptor Ectodomain Complexes by Circular Dichroic Spectroscopy Mol. Endocrinol., July 1, 2003; 17(7): 1192 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Fralish, P. Narayan, and D. Puett Consequences of Single-Chain Translation on the Structures of Two Chorionic Gonadotropin Yoked Analogs in {alpha}-{beta} and {beta}-{alpha} Configurations Mol. Endocrinol., April 1, 2003; 17(4): 757 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sohn, K. Ryu, G. Sievert, M. Jeoung, I. Ji, and T. H. Ji Follicle-stimulating Hormone Interacts with Exoloop 3 of the Receptor J. Biol. Chem., December 13, 2002; 277(51): 50165 - 50175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Narayan, J. Gray, and D. Puett Yoked Complexes of Human Choriogonadotropin and the Lutropin Receptor: Evidence that Monomeric Individual Subunits Are Inactive Mol. Endocrinol., December 1, 2002; 16(12): 2733 - 2745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamalainen, J. Kero, M. Poutanen, and I. Huhtaniemi Transgenic Mice Harboring Murine Luteinizing Hormone Receptor Promoter/{beta}-Galactosidase Fusion Genes: Different Structural and Hormonal Requirements of Expression in the Testis, Ovary, and Adrenal Gland Endocrinology, October 1, 2002; 143(10): 4096 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ji, C. Lee, Y. Song, P. M. Conn, and T. H. Ji Cis- and Trans-Activation of Hormone Receptors: the LH Receptor Mol. Endocrinol., June 1, 2002; 16(6): 1299 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lee, I. Ji, K. Ryu, Y. Song, P. M. Conn, and T. H. Ji Two Defective Heterozygous Luteinizing Hormone Receptors Can Rescue Hormone Action J. Biol. Chem., May 3, 2002; 277(18): 15795 - 15800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ascoli, F. Fanelli, and D. L. Segaloff The Lutropin/Choriogonadotropin Receptor, A 2002 Perspective Endocr. Rev., April 1, 2002; 23(2): 141 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Hawtin, V. J. Wesley, R. A. Parslow, J. Simms, A. Miles, K. McEwan, and M. Wheatley A Single Residue (Arg46) Located Within the N-Terminus of the V1a Vasopressin Receptor Is Critical for Binding Vasopressin But Not Peptide or Nonpeptide Antagonists Mol. Endocrinol., March 1, 2002; 16(3): 600 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Garcia-Campayo and I. Boime Independent Activities of FSH and LH Structurally Confined in a Single Polypeptide: Selective Modification of the Relative Potencies of the Hormones Endocrinology, December 1, 2001; 142(12): 5203 - 5211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kishi, X. Liu, T. Hirakawa, D. Reczek, A. Bretscher, and M. Ascoli Identification of Two Distinct Structural Motifs That, When Added to the C-Terminal Tail of the Rat LH Receptor, Redirect the Internalized Hormone-Receptor Complex from a Degradation to a Recycling Pathway Mol. Endocrinol., September 1, 2001; 15(9): 1624 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shinozaki, F. Fanelli, X. Liu, J. Jaquette, K. Nakamura, and D. L. Segaloff Pleiotropic Effects of Substitutions of a Highly Conserved Leucine in Transmembrane Helix III of the Human Lutropin/Choriogonadotropin Receptor with Respect to Constitutive Activation and Hormone Responsiveness Mol. Endocrinol., June 1, 2001; 15(6): 972 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Fralish, P. Narayan, and D. Puett High-Level Expression of a Functional Single-Chain Human Chorionic Gonadotropin-Luteinizing Hormone Receptor Ectodomain Complex in Insect Cells Endocrinology, April 1, 2001; 142(4): 1517 - 1524. [Abstract] [Full Text]
|
|
|
|
|
|
K. Angelova, P. Narayan, J. P. Simon, and D. Puett Functional Role of Transmembrane Helix 7 in the Activation of the Heptahelical Lutropin Receptor Mol. Endocrinol., April 1, 2000; 14(4): 459 - 471. [Abstract] [Full Text]
|
|
|
|
|
|
S. You, H. Kim, M. E. El Halawani, and D. N. Foster Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms II: Characterization of Differentially Regulated tLH-R Messenger Ribonucleic Acid Isoforms in the Ovary Biol Reprod, January 1, 2000; 62(1): 117 - 124. [Abstract] [Full Text]
|
|
|
|
|
|
J. B. M. Janosi, P. A. Ramsland, M. R. Mott, S. M. Firth, R. C. Baxter, and P. J. D. Delhanty The Acid-labile Subunit of the Serum Insulin-like Growth Factor-binding Protein Complexes. STRUCTURAL DETERMINATION BY MOLECULAR MODELING AND ELECTRON MICROSCOPY J. Biol. Chem., August 13, 1999; 274(33): 23328 - 23332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hong, I. Ji, and T. H. Ji The {beta}-Subunit of Human Choriogonadotropin Interacts with the Exodomain of the Luteinizing Hormone/Choriogonadotropin Receptor and Changes Its Interaction with the {alpha}-Subunit Mol. Endocrinol., August 1, 1999; 13(8): 1285 - 1294. [Abstract] [Full Text]
|
|
|
|
|
|
S. Hong, I. Ji, and T. H. Ji The {alpha}-Subunit of Human Choriogonadotropin Interacts with the Exodomain of the Luteinizing Hormone/Choriogonadotropin Receptor Endocrinology, June 1, 1999; 140(6): 2486 - 2493. [Abstract] [Full Text]
|
|
|
|
|
|
M. Tena-Sempere, P. R. Manna, and I. Huhtaniemi Molecular Cloning of the Mouse Follicle-Stimulating Hormone Receptor Complementary Deoxyribonucleic Acid: Functional Expression of Alternatively Spliced Variants and Receptor Inactivation by a C566T Transition in Exon 7 of the Coding Sequence Biol Reprod, June 1, 1999; 60(6): 1515 - 1527. [Abstract] [Full Text]
|
|
|
|
|
|
G. Han and D. R. Hampson Ligand Binding to the Amino-terminal Domain of the mGluR4 Subtype of Metabotropic Glutamate Receptor J. Biol. Chem., April 9, 1999; 274(15): 10008 - 10013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Alvarez, P. Narayan, J. Huang, and D. Puett Characterization of a Region of the Lutropin Receptor Extracellular Domain Near Transmembrane Helix 1 That Is Important in Ligand-Mediated Signaling Endocrinology, April 1, 1999; 140(4): 1775 - 1782. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ryu, R. L. Gilchrist, C.-S. Tung, I. Ji, and T. H. Ji High Affinity Hormone Binding to the Extracellular N-terminal Exodomain of the Follicle-stimulating Hormone Receptor Is Critically Modulated by Exoloop 3 J. Biol. Chem., October 30, 1998; 273(44): 28953 - 28958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hong, T. Phang, I. Ji, and T. H. Ji The Amino-terminal Region of the Luteinizing Hormone/Choriogonadotropin Receptor Contacts Both Subunits of Human Choriogonadotropin. I. MUTATIONAL ANALYSIS J. Biol. Chem., May 29, 1998; 273(22): 13835 - 13840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Phang, G. Kundu, S. Hong, I. Ji, and T. H. Ji The Amino-terminal Region of the Luteinizing Hormone/Choriogonadotropin Receptor Contacts Both Subunits of Human Choriogonadotropin. II. PHOTOAFFINITY LABELING J. Biol. Chem., May 29, 1998; 273(22): 13841 - 13847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Okamoto, N. Sekiyama, M. Otsu, Y. Shimada, A. Sato, S. Nakanishi, and H. Jingami Expression and Purification of the Extracellular Ligand Binding Region of Metabotropic Glutamate Receptor Subtype 1 J. Biol. Chem., May 22, 1998; 273(21): 13089 - 13096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. I. DeAlmeida and K. E. Mayo Identification of Binding Domains of the Growth Hormone-Releasing Hormone Receptor by Analysis of Mutant and Chimeric Receptor Proteins Mol. Endocrinol., May 1, 1998; 12(5): 750 - 765. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ryu, H. Lee, S. Kim, J. Beauchamp, C.-S. Tung, N. W. Isaacs, I. Ji, and T. H. Ji Modulation of High Affinity Hormone Binding. HUMAN CHORIOGONADOTROPIN BINDING TO THE EXODOMAIN OF THE RECEPTOR IS INFLUENCED BY EXOLOOP 2 OF THE RECEPTOR J. Biol. Chem., March 13, 1998; 273(11): 6285 - 6291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Osuga, M. Hayashi, M. Kudo, M. Conti, B. Kobilka, and A. J. W. Hsueh Co-expression of Defective Luteinizing Hormone Receptor Fragments Partially Reconstitutes Ligand-induced Signal Generation J. Biol. Chem., October 3, 1997; 272(40): 25006 - 25012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Biebermann, T. Schoneberg, H. Krude, G. Schultz, T. Gudermann, and A. Gruters Mutations of the Human Thyrotropin Receptor Gene Causing Thyroid Hypoplasia and Persistent Congenital Hypothyroidism J. Clin. Endocrinol. Metab., October 1, 1997; 82(10): 3471 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Osuga, M. Kudo, A. Kaipia, B. Kobilka, and A. J. W. Hsueh Derivation of Functional Antagonists Using N-Terminal Extracellular Domain of Gonadotropin and Thyrotropin Receptors Mol. Endocrinol., October 1, 1997; 11(11): 1659 - 1668. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Hashimoto, N. Ogawa, N. Hagihara, K. Yamamoto, K. Imanishi, H. Nogi, A. Nishino, T. Fujita, T. Matsuda, S. Nagata, et al. Vasoactive Intestinal Polypeptide and Pituitary Adenylate Cyclase-Activating Polypeptide Receptor Chimeras Reveal Domains That Determine Specificity of Vasoactive Intestinal Polypeptide Binding and Activation Mol. Pharmacol., July 1, 1997; 52(1): 128 - 135. [Abstract] [Full Text]
|
|
|
|
|
|
F.-P. Zhang, A. S. Rannikko, P. R. Manna, H. M. Fraser, and I. T. Huhtaniemi Cloning and Functional Expression of the Luteinizing Hormone Receptor Complementary Deoxyribonucleic Acid from the Marmoset Monkey Testis: Absence of Sequences Encoding Exon 10 in Other Species Endocrinology, June 1, 1997; 138(6): 2481 - 2490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Davis, T. G. Rozell, X. Liu, and D. L. Segaloff The Six N-linked Carbohydrates of the Lutropin/Choriogonadotropin Receptor Are Not Absolutely Required for Correct Folding, Cell Surface Expression, Hormone Binding, or Signal Transduction Mol. Endocrinol., May 1, 1997; 11(5): 550 - 562. [Abstract] [Full Text]
|
|
|
|
|
|
L. I. Lobel, P. Rausch, I. Trakht, S. Pollak, and J. W. Lustbader Filamentous Phage Displaying the Extracellular Domain of the hLH/CG Receptor Bind hCG Specifically Endocrinology, March 1, 1997; 138(3): 1232 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Beau, M. Misrahi, B. Gross, B. Vannier, H. Loosfelt, M. T. V. Hai, C. Pichon, and E. Milgrom Basolateral Localization and Transcytosis of Gonadotropin and Thyrotropin Receptors Expressed in Madin-Darby Canine Kidney Cells J. Biol. Chem., February 21, 1997; 272(8): 5241 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hong, K.-S. Ryu, M.-S. Oh, I. Ji, and T. H. Ji Roles of Transmembrane Prolines and Proline-induced Kinks of the Lutropin/Choriogonadotropin Receptor J. Biol. Chem., February 14, 1997; 272(7): 4166 - 4171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jaquette and D. L. Segaloff Temperature Sensitivity of Some Mutants of the Lutropin/Choriogonadotropin Receptor Endocrinology, January 1, 1997; 138(1): 85 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wu, P. Narayan, and D. Puett Protein Engineering of a Novel Constitutively Active Hormone-Receptor Complex J. Biol. Chem., December 6, 1996; 271(49): 31638 - 31642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Gilchrist, K.-S. Ryu, I. Ji, and T. H. Ji The Luteinizing Hormone/Chorionic Gonadotropin Receptor Has Distinct Transmembrane Conductors for cAMP and Inositol Phosphate Signals J. Biol. Chem., August 9, 1996; 271(32): 19283 - 19287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-S. Ryu, R. L. Gilchrist, I. Ji, S.-J. Kim, and T. H. Ji Exoloop 3 of the Luteinizing Hormone/Choriogonadotropin Receptor J. Biol. Chem., March 29, 1996; 271(13): 7301 - 7304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhang, E. Buczko, and M. L. Dufau Requirement of Cysteine Residues in Exons 1-6 of the Extracellular Domain of the Luteinizing Hormone Receptor for Gonadotropin Binding J. Biol. Chem., March 8, 1996; 271(10): 5755 - 5760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Abell, X. Liu, and D. L. Segaloff Deletions of Portions of the Extracellular Loops of the Lutropin/Choriogonadotropin Receptor Decrease the Binding Affinity for Ovine Luteinizing Hormone, but Not Human Choriogonadotropin, by Preventing the Formation of Mature Cell Surface Receptor J. Biol. Chem., February 23, 1996; 271(8): 4518 - 4527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Fernandez and D. Puett Lys[IMAGE] in the Third Extracellular Loop of the Lutropin/Choriogonadotropin Receptor Is Critical for Signaling J. Biol. Chem., January 12, 1996; 271(2): 925 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang and D. Puett Identification of Two Amino Acid Residues on the Extracellular Domain of the Lutropin/Choriogonadotropin Receptor Important in Signaling J. Biol. Chem., December 15, 1995; 270(50): 30023 - 30028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Moyle, R. K. Campbell, S. N. V. Rao, N. G. Ayad, M. P. Bernard, Y. Han, and Y. Wang Model of Human Chorionic Gonadotropin and Lutropin Receptor Interaction That Explains Signal Transduction of the Glycoprotein Hormones J. Biol. Chem., August 25, 1995; 270(34): 20020 - 20031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ji and T. H. Ji Differential Roles of Exoloop 1 of the Human Follicle-stimulating Hormone Receptor in Hormone Binding and Receptor Activation J. Biol. Chem., July 7, 1995; 270(27): 15970 - 15973. [Abstract] [Full Text] [PDF] |
