| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 35, 21561-21566, 12, 1990
JD Scott, RE Stofko, JR McDonald, JD Comer, EA Vitalis and JA Mangili
The type II cAMP-dependent protein kinase (PKA) is localized to specific
subcellular environments through binding of dimeric regulatory subunits
(RII) to anchoring proteins. Cytoskeletal localization occurs through RII
dimer interaction with the PKA substrate molecule microtubule-associated
protein 2 (MAP2). RII alpha deletion mutants and RII alpha/endonexin
chimeras retained MAP2 binding activity if they contained the first 79
residues of the molecule. Disruption of RII alpha dimerization always
prevented MAP2 interaction because 1) RII delta 1-14 (an amino-terminal
deletion mutant lacking residues 1-14) was unable to bind MAP2 or form
dimers, and 2) a modified RII alpha monomer including residues 1-14 did not
bind MAP2. Chimeric proteins containing the first 30 residues of RII alpha
fused to endonexin II formed dimers but did not bind MAP2. This suggested
other side-chains between residues 30-79 also participate in MAP2
interaction. Peptide studies indicate additional contact with MAP2 may
occur through an acidic region (residues 68-82) close to the RII
autoinhibitor domain. Therefore, anchored PKA holoenzyme topology may
position the catalytic subunit and MAP2 as to allow its preferential
phosphorylation upon kinase activation.
Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase
Vollum Institute for Advanced Biomedical Research, Portland, Oregon 97201.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. M. Goldenberg, E. A. Rossi, R. M. Sharkey, W. J. McBride, and C.-H. Chang Multifunctional Antibodies by the Dock-and-Lock Method for Improved Cancer Imaging and Therapy by Pretargeting J. Nucl. Med., January 1, 2008; 49(1): 158 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hlavanda, E. Klement, E. Kokai, J. Kovacs, O. Vincze, N. Tokesi, F. Orosz, K. F. Medzihradszky, V. Dombradi, and J. Ovadi Phosphorylation Blocks the Activity of Tubulin Polymerization-promoting Protein (TPPP): IDENTIFICATION OF SITES TARGETED BY DIFFERENT KINASES J. Biol. Chem., October 5, 2007; 282(40): 29531 - 29539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Chang, E. A. Rossi, and D. M. Goldenberg The Dock and Lock Method: A Novel Platform Technology for Building Multivalent, Multifunctional Structures of Defined Composition with Retained Bioactivity Clin. Cancer Res., September 15, 2007; 13(18): 5586s - 5591s. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Rossi, D. M. Goldenberg, T. M. Cardillo, W. J. McBride, R. M. Sharkey, and C.-H. Chang Stably tethered multifunctional structures of defined composition made by the dock and lock method for use in cancer targeting PNAS, May 2, 2006; 103(18): 6841 - 6846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Sayner, M. Alexeyev, C. W. Dessauer, and T. Stevens Soluble Adenylyl Cyclase Reveals the Significance of cAMP Compartmentation on Pulmonary Microvascular Endothelial Cell Barrier Circ. Res., March 17, 2006; 98(5): 675 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. Collins, L. Yu, M. P. Coba, H. Husi, I. Campuzano, W. P. Blackstock, J. S. Choudhary, and S. G. N. Grant Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins J. Biol. Chem., February 18, 2005; 280(7): 5972 - 5982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Salvador, M. P. Flynn, J. Avila, S. Reierstad, E. T. Maizels, H. Alam, Y. Park, J. D. Scott, D. W. Carr, and M. Hunzicker-Dunn Neuronal Microtubule-associated Protein 2D Is a Dual A-kinase Anchoring Protein Expressed in Rat Ovarian Granulosa Cells J. Biol. Chem., June 25, 2004; 279(26): 27621 - 27632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Elliott, R. A. Shanks, I. U. Khan, J. W. Brooks, P. J. Burkett, B. J. Nelson, V. Kyttaris, Y.-T. Juang, G. C. Tsokos, and G. M. Kammer Down-Regulation of IL-2 Production in T Lymphocytes by Phosphorylated Protein Kinase A-RII{beta} J. Immunol., June 15, 2004; 172(12): 7804 - 7812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Elliott, M. Tolnay, G. C. Tsokos, and G. M. Kammer Protein Kinase A Regulatory Subunit Type II{beta} Directly Interacts with and Suppresses CREB Transcriptional Activity in Activated T Cells J. Immunol., October 1, 2003; 171(7): 3636 - 3644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Brown, K. Miki, D. B. Harper, and E. M. Eddy A-Kinase Anchoring Protein 4 Binding Proteins in the Fibrous Sheath of the Sperm Flagellum Biol Reprod, June 1, 2003; 68(6): 2241 - 2248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Aoubala, J. Holt, R. A. Clegg, D. J. Rowlands, and M. Harris The inhibition of cAMP-dependent protein kinase by full-length hepatitis C virus NS3/4A complex is due to ATP hydrolysis J. Gen. Virol., July 1, 2001; 82(7): 1637 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. H. Woo, S. N. Duffy, T. Abel, and P. V. Nguyen Genetic and Pharmacological Demonstration of Differential Recruitment of cAMP-Dependent Protein Kinases by Synaptic Activity J Neurophysiol, December 1, 2000; 84(6): 2739 - 2745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurihara, N. Nakanishi, and T. Ueha Regulation of Na+-K+-ATPase by cAMP-dependent protein kinase anchored on membrane via its anchoring protein Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1516 - C1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Fujita, K Nakamura, T Kato, N Watanabe, T Ishizaki, K Kimura, A Mizoguchi, and S Narumiya Ropporin, a sperm-specific binding protein of rhophilin, that is localized in the fibrous sheath of sperm flagella J. Cell Sci., January 1, 2000; 113(1): 103 - 112. [Abstract] [PDF]
|
|
|
|
 |
|
 K. W. Trotter, I. D.C. Fraser, G. K. Scott, M. J. Stutts, J. D. Scott, and S. L. Milgram Alternative Splicing Regulates the Subcellular Localization of A-kinase Anchoring Protein 18 Isoforms J. Cell Biol., December 27, 1999; 147(7): 1481 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miki and E. M. Eddy Single Amino Acids Determine Specificity of Binding of Protein Kinase A Regulatory Subunits by Protein Kinase A Anchoring Proteins J. Biol. Chem., October 8, 1999; 274(41): 29057 - 29062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, E. A. Rossi, J. D. Hoheisel, D. Kalderon, and C. S. Rubin Generation of a Novel A Kinase Anchor Protein and a Myristoylated Alanine-rich C Kinase Substrate-like Analog from a Single Gene J. Biol. Chem., September 17, 1999; 274(38): 27191 - 27200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Banky, L. J.-S. Huang, and S. S. Taylor Dimerization/Docking Domain of the Type Ialpha Regulatory Subunit of cAMP-dependent Protein Kinase. REQUIREMENTS FOR DIMERIZATION AND DOCKING ARE DISTINCT BUT OVERLAPPING J. Biol. Chem., December 25, 1998; 273(52): 35048 - 35055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ali, X. Chen, M. Lu, J. Z. Xu, K. M. Lerea, S. C. Hebert, and W. Wang The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels PNAS, August 18, 1998; 95(17): 10274 - 10278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gibson and S. S. Taylor Dissecting the Cooperative Reassociation of the Regulatory and Catalytic Subunits of cAMP-dependent Protein Kinase. ROLE OF Trp-196 IN THE CATALYTIC SUBUNIT J. Biol. Chem., December 19, 1997; 272(51): 31998 - 32005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Leon, F. W. Herberg, P. Banky, and S. S. Taylor A Stable alpha -Helical Domain at the N Terminus of the RIalpha Subunits of cAMP-dependent Protein Kinase Is a Novel Dimerization/Docking Motif J. Biol. Chem., November 7, 1997; 272(45): 28431 - 28437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J.-s. Huang, K. Durick, J. A. Weiner, J. Chun, and S. S. Taylor D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain PNAS, October 14, 1997; 94(21): 11184 - 11189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Newlon, M. Roy, Z. E. Hausken, J. D. Scott, and P. A. Jennings The A-kinase Anchoring Domain of Type IIalpha cAMP-dependent Protein Kinase Is Highly Helical J. Biol. Chem., September 19, 1997; 272(38): 23637 - 23644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Dell'Acqua and J. D. Scott Protein Kinase A Anchoring J. Biol. Chem., May 16, 1997; 272(20): 12881 - 12884. [Full Text] [PDF]
|
|
|
|
|
|
L. J.-s. Huang, K. Durick, J. A. Weiner, J. Chun, and S. S. Taylor Identification of a Novel Protein Kinase A Anchoring Protein That Binds Both Type I and Type II Regulatory Subunits J. Biol. Chem., March 21, 1997; 272(12): 8057 - 8064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Gray, V. C. Tibbs, W. A. Catterall, and B. J. Murphy Identification of a 15-kDa cAMP-dependent Protein Kinase-anchoring Protein Associated with Skeletal Muscle L-type Calcium Channels J. Biol. Chem., March 7, 1997; 272(10): 6297 - 6302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vijayaraghavan, S. A. Goueli, M. P. Davey, and D. W. Carr Protein Kinase A-anchoring Inhibitor Peptides Arrest Mammalian Sperm Motility J. Biol. Chem., February 21, 1997; 272(8): 4747 - 4752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. E. Hausken, M. L. Dell'Acqua, V. M. Coghlan, and J. D. Scott Mutational Analysis of the A-Kinase Anchoring Protein (AKAP)-binding Site on RII. CLASSIFICATION OF SIDE CHAIN DETERMINANTS FOR ANCHORING AND ISOFORM SELECTIVE ASSOCIATION WITH AKAPs J. Biol. Chem., November 15, 1996; 271(46): 29016 - 29022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. McCartney, B. M. Little, L. K. Langeberg, and J. D. Scott Cloning and Characterization of A-kinase Anchor Protein 100 (AKAP100) J. Biol. Chem., April 21, 1995; 270(16): 9327 - 9333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Mochly-Rosen Localization of protein kinases by anchoring proteins: a theme in signal transduction Science, April 14, 1995; 268(5208): 247 - 251. [Abstract] [PDF]
|
|
|
|
|
|
Y. Li and C. S. Rubin Mutagenesis of the Regulatory Subunit (RIIbeta) of cAMP-dependent Protein Kinase IIbeta Reveals Hydrophobic Amino Acids That Are Essential for RIIbeta Dimerization and/or Anchoring RIIbeta to the Cytoskeleton J. Biol. Chem., January 27, 1995; 270(4): 1935 - 1944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bacskai, B Hochner, M Mahaut-Smith, Adams SR, B. Kaang, E. Kandel, and R. Tsien Spatially resolved dynamics of cAMP and protein kinase A subunits in Aplysia sensory neurons Science, April 9, 1993; 260(5105): 222 - 226. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
