HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jakobs, E. S. Articles by Paterson, A. R. Search for Related Content PubMed PubMed Citation Articles by Jakobs, E. S. Articles by Paterson, A. R. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 36, 22210-22216, Dec, 1990

Expression of sodium-linked nucleoside transport activity in monolayer cultures of IEC-6 intestinal epithelial cells

ES Jakobs, DJ Van Os-Corby and AR Paterson
McEachern Laboratory, University of Alberta, Edmonton, Canada.

Mature, confluent monolayer cultures of IEC-6 rat intestinal epithelial cells in conventional growth media express both Na(+)-linked, concentrative nucleoside transport (NT) activity and equilibrative, inhibitor-sensitive NT activity, but do not show morphologic differentiation. Na(+)-dependent fluxes of Ado and formycin B were minor in early subconfluent IEC-6 monolayers, but increased severalfold to become the major component of influx of these agents in confluent monolayers grown in medium containing Nu-Serum, a commercial medium supplement with a low serum content. In monolayers cultured in medium with fetal bovine serum, cell proliferation rates were similar to those in medium supplemented with Nu-Serum, but expression of Na(+)-linked NT activity was 6-8-fold lower than in monolayers grown in the latter medium. Inclusion of hydrocortisone in growth medium with Nu-Serum caused a 2-fold increase in the expression of Na(+)-linked NT activity. Experiments in which components of medium supplementation were withheld showed that insulin and epidermal growth factor were important in expression of the Na(+)-linked NT activity. Because the Na(+)-linked NT system has a brush border location in fresh intestinal epithelium, it is concluded that the regulated expression of this activity in the IEC- 6 monolayers is a differentiative change.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. Aymerich, M. Pastor-Anglada, and F. J. Casado Long Term Endocrine Regulation of Nucleoside Transporters in Rat Intestinal Epithelial Cells J. Gen. Physiol., October 25, 2004; 124(5): 505 - 512. [Abstract] [Full Text] [PDF]

				T. Pawelczyk, M. Podgorska, and M. Sakowicz The Effect of Insulin on Expression Level of Nucleoside Transporters in Diabetic Rats Mol. Pharmacol., January 1, 2003; 63(1): 81 - 88. [Abstract] [Full Text] [PDF]

				G. Lee, S. Dallas, M. Hong, and R. Bendayan Drug Transporters in the Central Nervous System: Brain Barriers and Brain Parenchyma Considerations Pharmacol. Rev., December 1, 2001; 53(4): 569 - 596. [Abstract] [Full Text] [PDF]

				S. A. Flanagan and K. A. Meckling-Gill Characterization of a Novel Na+-dependent, Guanosine-specific, Nitrobenzylthioinosine-sensitive Transporter in Acute Promyelocytic Leukemia Cells J. Biol. Chem., July 18, 1997; 272(29): 18026 - 18032. [Abstract] [Full Text] [PDF]