| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 36, 22228-22237, Dec, 1990
E Suenson, P Bjerrum, A Holm, B Lind, M Meldal, J Selmer and LC Petersen
Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
When thrombin-mediated fibrin formation and tissue plasminogen activator (t-PA)-mediated fibrinolysis proceed in dynamic interaction, desA-(desB beta 1-42)-fragment X polymers are shown to be the predominant fibrin derivatives present during the rapid second phase of Glu1- and Lys78-plasminogen activation. To further investigate the effect of this intermediate, a method was developed for the production and purification of fibrinogen-derived desA-(desB beta 1-42)-fragment X, deprived of both COOH-terminal A alpha-chains, but still capable of thrombin-mediated polymerization. DesA-(desB beta 1-42)-fragment X polymer was compared to intact fibrin with regard to its stimulatory effect on Glu1-, Lys78-, and Val443-plasminogen activation, and its binding of Glu1- and Lys78-plasminogen. Pure fragment X polymer gave rise to a biphasic activation pattern like that of fibrin, demonstrating similar kinetics of rapid phase activation. The dissociation constant for the binding of plasminogen to the effector decreases by a factor of 14, and the stoichiometry increases by a factor of 2 upon plasmin-catalyzed cleavage of both native Glu1- to Lys78-plasminogen, and fibrin to fragment X polymer. We conclude that desA-fibrin protofibril formation is sufficient to initiate fibrin enhancement of t-PA-catalyzed plasminogen activation, and that optimal stimulation depends on further plasmin-mediated modification of the fibrin effector to desA-fragment X-related moieties. Optimal stimulation is dependent on the presence of the kringle 1-4 domains of plasminogen and probably results from altered and increased binding of both plasminogen and t-PA to the modified effector.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. M. Binette, F. B. Taylor Jr, G. Peer, and L. Bajzar Thrombin-thrombomodulin connects coagulation and fibrinolysis: more than an in vitro phenomenon Blood, November 1, 2007; 110(9): 3168 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Walker and L. Bajzar The Intrinsic Threshold of the Fibrinolytic System Is Modulated by Basic Carboxypeptidases, but the Magnitude of the Antifibrinolytic Effect of Activated Thrombin-activable Fibrinolysis Inhibitor Is Masked by Its Instability J. Biol. Chem., July 2, 2004; 279(27): 27896 - 27904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. MATSUDA and T. SUGO Hereditary Disorders of Fibrinogen Ann. N.Y. Acad. Sci., June 1, 2001; 936(1): 65 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-E. DEMPFLE, S. ARGIRIOU, S. ALESCI, K. KUCHER, H. MULLER-PELTZER, K. RUBSAMEN, and D. L. HEENE Fibrin Formation and Proteolysis during Ancrod Treatment: Evidence for Des-A-Profibrin Formation and Thrombin Independent Factor XIII Activity Ann. N.Y. Acad. Sci., June 1, 2001; 936(1): 210 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, M. B. Boffa, L. Bajzar, J. B. Walker, and M. E. Nesheim A Study of the Mechanism of Inhibition of Fibrinolysis by Activated Thrombin-activable Fibrinolysis Inhibitor J. Biol. Chem., October 16, 1998; 273(42): 27176 - 27181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C.F. Cote, S. T. Lord, and K. P. Pratt gamma -Chain Dysfibrinogenemias: Molecular Structure-Function Relationships of Naturally Occurring Mutations in the gamma Chain of Human Fibrinogen Blood, October 1, 1998; 92(7): 2195 - 2212. [Full Text] [PDF]
|
|
|
|
|
|
G. Kassam, K.-S. Choi, J. Ghuman, H.-M. Kang, S. L. Fitzpatrick, T. Zackson, S. Zackson, M. Toba, A. Shinomiya, and D. M. Waisman The Role of Annexin II Tetramer in the Activation of Plasminogen J. Biol. Chem., February 20, 1998; 273(8): 4790 - 4799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Asakura, K. Niwa, T. Tomozawa, Y.-m. Jin, S. Madoiwa, Y. Sakata, T. Sakai, H. Funayama, G. Soe, F. Forgerty, et al. Fibroblasts Spread on Immobilized Fibrin Monomer by Mobilizing a beta 1-class Integrin, Together with a Vitronectin Receptor alpha vbeta 3 on Their Surface J. Biol. Chem., March 28, 1997; 272(13): 8824 - 8829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J.G. Horrevoets, H. Pannekoek, and M. E. Nesheim Production and Characterization of Recombinant Human Plasminogen(S741C-Fluorescein). A NOVEL APPROACH TO STUDY ZYMOGEN ACTIVATION WITHOUT GENERATION OF ACTIVE PROTEASE J. Biol. Chem., January 24, 1997; 272(4): 2176 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. V. Sakharov and D. C. Rijken Superficial Accumulation of Plasminogen During Plasma Clot Lysis Circulation, October 1, 1995; 92(7): 1883 - 1890. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. G. Horrevoets, A. E. Smilde, J. C. Fredenburgh, H. Pannekoek, and M. E. Nesheim The Activation-resistant Conformation of Recombinant Human Plasminogen Is Stabilized by Basic Residues in the Amino-terminal Hinge Region J. Biol. Chem., June 30, 1995; 270(26): 15770 - 15776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-S. Choi, S. L. Fitzpatrick, N. R. Filipenko, D. K. Fogg, G. Kassam, A. M. Magliocco, and D. M. Waisman Regulation of Plasmin-dependent Fibrin Clot Lysis by Annexin II Heterotetramer J. Biol. Chem., June 29, 2001; 276(27): 25212 - 25221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Urano, H. Ihara, K. Umemura, Y. Suzuki, M. Oike, S. Akita, Y. Tsukamoto, I. Suzuki, and A. Takada The Profibrinolytic Enzyme Subtilisin NAT Purified from Bacillus subtilis Cleaves and Inactivates Plasminogen Activator Inhibitor Type 1 J. Biol. Chem., June 29, 2001; 276(27): 24690 - 24696. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
