HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spriggs, M. K. Articles by Bauer, J. Search for Related Content PubMed PubMed Citation Articles by Spriggs, M. K. Articles by Bauer, J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 36, 22499-22505, 12, 1990

Induction of an interleukin-1 receptor (IL-1R) on monocytic cells. Evidence that the receptor is not encoded by a T cell-type IL-1R mRNA

MK Spriggs, PJ Lioubin, J Slack, SK Dower, U Jonas, D Cosman, JE Sims and J Bauer
Immunex Research and Development Corporation, Seattle, Washington 98101.

Primary human monocytes and the human monocytic cell line THP-1 were induced to express receptors for interleukin-1 alpha (IL-1 alpha) and IL-1 beta. Treatment of primary monocytes with dexamethasone resulted in a 10-fold increase in receptor number over untreated cells, to approximately 2,000 receptors/cell. Treatment of THP-1 cells with phorbol ester followed by prostaglandin E2 and dexamethasone resulted in the expression of approximately 30,000 receptors/cell. Competitive binding assays on THP-1 cells showed that both IL-1 alpha and IL-1 beta bind to the same receptor. The monocyte IL-1R is significantly smaller (63 kDa) than the T cell IL-1R (80 kDa) and is immunologically distinct. However, induction of monocytes and monocytic cell lines leads to the appearance of an abundant mRNA of approximately 5,000 bases which hybridizes to a cDNA probe from the T cell-type IL-1R. Sequence data obtained from a cDNA clone of this mRNA indicate that the message is identical to the T cell IL-1R mRNA throughout the coding region. A smaller mRNA, also homologous to the T cell IL-1R mRNA, accumulated in induced THP-1 cells and has a shorter 3'-untranslated region than the larger. Data are presented which suggest that neither form of this message encodes the 63-kDa IL-1R, but rather that this protein is the product of a separate nonhomologous mRNA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. L. Cubitt, R. N. Lausch, and J. E. Oakes Synthesis of Type II Interleukin-1 Receptors by Human Corneal Epithelial Cells but not by Keratocytes Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 701 - 704. [Abstract] [Full Text]

				K F Chung and P J Barnes Cytokines in asthma Thorax, September 1, 1999; 54(9): 825 - 857. [Full Text]

				S. Valles, C. Tsoi, W.-Y. Huang, D. Wyllie, F. Carlotti, J. A. Askari, M. J. Humphries, S. K. Dower, and E. E. Qwarnstrom Recruitment of a Heparan Sulfate Subunit to the Interleukin-1 Receptor Complex. REGULATION BY FIBRONECTIN ATTACHMENT J. Biol. Chem., July 16, 1999; 274(29): 20103 - 20109. [Abstract] [Full Text] [PDF]

				G. Penton-Rol, S. Orlando, N. Polentarutti, S. Bernasconi, M. Muzio, M. Introna, and A. Mantovani Bacterial Lipopolysaccharide Causes Rapid Shedding, Followed by Inhibition of mRNA Expression, of the IL-1 Type II Receptor, with Concomitant Up-Regulation of the Type I Receptor and Induction of Incompletely Spliced Transcripts J. Immunol., March 1, 1999; 162(5): 2931 - 2938. [Abstract] [Full Text] [PDF]

				P. J. Barnes, K. F. Chung, and C. P. Page Inflammatory Mediators of Asthma: An Update Pharmacol. Rev., December 1, 1998; 50(4): 515 - 596. [Abstract] [Full Text] [PDF]

				M. Eller, M Yaar, K Ostrom, D. Harkness, and B. Gilchrest A role for interleukin-1 in epidermal differentiation: regulation by expression of functional versus decoy receptors J. Cell Sci., January 8, 1995; 108(8): 2741 - 2746. [Abstract] [PDF]

				R. Allen, R. Armitage, M. Conley, H Rosenblatt, N. Jenkins, N. Copeland, M. Bedell, S Edelhoff, C. Disteche, D. Simoneaux, et al. CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome Science, February 12, 1993; 259(5097): 990 - 993. [Abstract]

				L. Li, S. Cousart, J. Hu, and C. E. McCall Characterization of Interleukin-1 Receptor-associated Kinase in Normal and Endotoxin-tolerant Cells J. Biol. Chem., July 21, 2000; 275(30): 23340 - 23345. [Abstract] [Full Text] [PDF]