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J. Biol. Chem., Vol. 265, Issue 6, 3022-3029, Feb, 1990
K Liberek, J Osipiuk, M Zylicz, D Ang, J Skorko and C Georgopoulos
The process of initiation of lambda DNA replication requires the assembly
of the proper nucleoprotein complex at the origin of replication, ori
lambda. The complex is composed of both phage and host- coded proteins. The
lambda O initiator protein binds specifically to ori lambda. The lambda P
initiator protein binds to both lambda O and the host-coded dnaB helicase,
giving rise to an ori lambda DNA.lambda O.lambda P.dnaB structure. The dnaK
and dnaJ heat shock proteins have been shown capable of dissociating this
complex. The thus freed dnaB helicase unwinds the duplex DNA template at
the replication fork. In this report, through cross-linking, size
chromatography, and protein affinity chromatography, we document some of
the protein-protein interactions occurring at ori lambda. Our results show
that the dnaK protein specifically interacts with both lambda O and lambda
P, and that the dnaJ protein specifically interacts with the dnaB helicase.
Physical interactions between bacteriophage and Escherichia coli proteins required for initiation of lambda DNA replication
Department of Molecular Biology, University of Gdansk, Poland.
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