HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hadjiagapiou, C. Articles by Sprecher, H. Search for Related Content PubMed PubMed Citation Articles by Hadjiagapiou, C. Articles by Sprecher, H. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 8, 4369-4373, Mar, 1990

Metabolism of 15-hydroxy-5,8,11,13-eicosatetraenoic acid by MOLT-4 cells and blood T-lymphocytes

C Hadjiagapiou, JB Travers, RH Fertel and H Sprecher
Department of Physiological Chemistry, Ohio State University, Columbus 43210.

MOLT-4 lymphocytes metabolize 15-hydroxy-5,8,11,13-eicosatetraenoic acid (15-HETE) via beta-oxidation with retention of the hydroxyl group at the omega 6-carbon atom. 15-HETE oxidation is accompanied by the time-dependent accumulation of both beta-hydroxy acids and metabolites produced by repetitive cycles of the beta-oxidation spiral. Detection of 7-hydroxy-5-dodecenoic acid shows that these cells continue to beta- oxidize the substrate when the conjugated diene is allylic to a hydroxyl group. When 15-HETE was the substrate, it was also possible to detect 12-hydroxy-5,8,10-heptadecatrien-1-al and 3,15-dihydroxy-8,11,13- eicosatrienoic acid. The former product may be produced by alpha- oxidation of 13-hydroxy-6,9,11-octadecatrienoic acid followed by its decarboxylation. Detection of a 20-carbon metabolite, lacking a double bond at position 5, suggests that an intermediate of beta-oxidation was used as a substrate for chain elongation. When 13-hydroxy-6,9,11- octadecatrienoic acid was used as a substrate, it was indeed possible to detect 3,15-dihydroxy-8,11,13-eicosatrienoic acid as well as 15- hydroxy-8,11,13-eicosatrienoic acid. In addition, 13-hydroxy-6,9,11- octadecatrienoic acid was a precursor for the biosynthesis of both 14- hydroxy-7,10,12-nonadecatrien-1-al and 1,14-dihydroxy-7,10,12- nonadecatriene. These studies with MOLT-4 cells as well as with T- lymphocytes isolated from blood show that products of the 15- lipoxygenase pathway are metabolized with the accumulation of a variety of compounds. Since 15-HETE has been implicated as a modulator of T- cell function, these findings raise the possibility that the newly described metabolites may be involved in regulating lymphocyte function.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. L. Luthria, S. P. Baykousheva, and H. Sprecher Double Bond Removal from Odd-numbered Carbons during Peroxisomal [IMAGE]-Oxidation of Arachidonic Acid Requires both 2,4-Dienoyl-CoA Reductase and [IMAGE][IMAGE],[IMAGE][IMAGE]-Dienoyl-CoA Isomerase J. Biol. Chem., June 9, 1995; 270(23): 13771 - 13776. [Abstract] [Full Text] [PDF]