J. Biol. Chem., Vol. 265, Issue 9, 4839-4843, Mar, 1990

DNA methylation and collagen IV gene expression in F9 teratocarcinoma cells

PD Burbelo, S Horikoshi and Y Yamada
Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.

Although undifferentiated F9 teratocarcinoma cells express low levels of mRNAs for collagen IV, previous transient transfection experiments using an alpha 1(IV) collagen gene promoter-enhancer-CAT construct revealed a high level of transcriptional activity in these cells. In this report, we find that when this construct is introduced stably into undifferentiated F9 teratocarcinoma cells, expression does not occur unless the cells are treated with retinoic acid and dibutyryl-cAMP. Such activation mimics the endogenous gene activity. Treatment of the cells containing the integrated construct with 5-azacytidine, an agent which prevents DNA methylation, also activates transcription and acts synergistically with retinoic acid and cAMP. Analysis of DNA isolated from F9 teratocarcinoma cells revealed that there was a specific demethylation of the DNA within the 5'-flanking region of the collagen IV genes following treatment with retinoic acid and cAMP. These results suggest that during differentiation, DNA demethylation may play an important role in transcriptional regulation of the collagen IV genes.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Ikeda, K.-i. Iyama, N. Ishikawa, H. Egami, M. Nakao, Y. Sado, Y. Ninomiya, and H. Baba Loss of Expression of Type IV Collagen {alpha}5 and {alpha}6 Chains in Colorectal Cancer Associated with the Hypermethylation of Their Promoter Region Am. J. Pathol., March 1, 2006; 168(3): 856 - 865. [Abstract] [Full Text] [PDF]

				H. Fujita, R. Fujii, S. Aratani, T. Amano, A. Fukamizu, and T. Nakajima Antithetic Effects of MBD2a on Gene Regulation Mol. Cell. Biol., April 15, 2003; 23(8): 2645 - 2657. [Abstract] [Full Text] [PDF]

				J.-H. Cho, H. Kimura, T. Minami, J. Ohgane, N. Hattori, S. Tanaka, and K. Shiota DNA Methylation Regulates Placental Lactogen I Gene Expression Endocrinology, August 1, 2001; 142(8): 3389 - 3396. [Abstract] [Full Text] [PDF]

				B. Zhu, D. Benjamin, Y. Zheng, H. Angliker, S. Thiry, M. Siegmann, and J.-P. Jost Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene PNAS, April 5, 2001; (2001) 91097298. [Abstract] [Full Text]

				A. Schmitz, M. Short, O. Ammerpohl, C. Asbrand, J. Nickel, and R. Renkawitz Cis-elements Required for the Demethylation of the Mouse M-lysozyme Downstream Enhancer J. Biol. Chem., August 15, 1997; 272(33): 20850 - 20856. [Abstract] [Full Text] [PDF]

				P. S. Thorner, K. Zheng, R. Kalluri, R. Jacobs, and B. G. Hudson Coordinate Gene Expression of the alpha 3, alpha 4, and alpha 5 Chains of Collagen Type IV. EVIDENCE FROM A CANINE MODEL OF X-LINKED NEPHRITIS WITH A COL4A5 GENE MUTATION J. Biol. Chem., June 7, 1996; 271(23): 13821 - 13828. [Abstract] [Full Text] [PDF]

				B. Zhu, D. Benjamin, Y. Zheng, H. Angliker, S. Thiry, M. Siegmann, and J.-P. Jost Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene PNAS, April 24, 2001; 98(9): 5031 - 5036. [Abstract] [Full Text] [PDF]