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J. Biol. Chem., Vol. 266, Issue 11, 6830-6833, 04, 1991
SK Batra, RS Metzgar and MA Hollingsworth
A cDNA library from a poorly differentiated human pancreatic tumor cell
line was screened for differentially expressed mRNAs using single- stranded
cDNA probes synthesized from poly(A+) RNA of the poorly differentiated cell
line Panc 1 and a very well differentiated cell line CD11. One of the cDNA
clones isolated hybridized to a transcript size of 650 base pairs on
Northern blot analysis and showed 30-fold higher expression in the poorly
differentiated cell line as compared with the well differentiated cell
line. Sequence analysis of this cDNA clone and its deduced amino acid
sequence showed an open reading frame of 441 nucleotides with 100 and 98.6%
homology to ribosomal protein S16 (rpS16) from rat and mouse, respectively.
Northern blot analyses with a panel of 14 pancreatic cell lines, 2 breast
cell lines, 2 colon cell lines, and several other tissues showed higher
expression of rpS16 only in the poorly differentiated pancreatic tumor cell
line Panc 1. The expression of mRNA for two other ribosomal proteins, rpL30
and rpL32, were not elevated in Panc 1. Southern blot analysis of genomic
DNA showed a 20-fold amplification of a single band among the rpS16 family
only in the Panc 1 cell line.
Molecular cloning and sequence analysis of the human ribosomal protein S16
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
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