JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brooks, A. R.
Right arrow Articles by Levy-Wilson, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brooks, A. R.
Right arrow Articles by Levy-Wilson, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 266, Issue 12, 7848-7859, 04, 1991

Characterization of tissue-specific enhancer elements in the second intron of the human apolipoprotein B gene

AR Brooks, BD Blackhart, K Haubold and B Levy-Wilson
Gladstone Foundation Laboratories for Cardiovascular Disease, Cardiovascular Research Institute, San Francisco, California 94140-0608.

We report the identification and characterization of tissue-specific transcriptional enhancer elements that influence the expression of the human apolipoprotein B gene. A 704-base pair PstI fragment comprising sequences from the first and second introns of the human apolipoprotein B gene (positions +360 to +1064) possesses tissue-specific transcriptional enhancer elements when assayed in transient transfection experiments using either the apolipoprotein B or thymidine kinase promoter. The majority of the enhancer activity, which was observed in transcriptionally active HepG2 and CaCo-2 cells, but not in transcriptionally inactive Chinese hamster ovary or HeLa cells, was subsequently localized to a 443-base pair SmaI-PvuII fragment (positions +621 to +1064) within the second intron of the apolipoprotein B gene. Gel retention experiments demonstrated that sequence motifs within this region interact with a number of nuclear proteins from HepG2, CaCo-2, and HeLa cells. The actual sequence elements that bound to nuclear proteins from HepG2 cells were identified by DNase I footprinting. Deletion experiments were performed to distinguish those protein-binding regions involved in the enhancer effect. Our data demonstrate that sequences between positions +806 and +940 are essential for this enhancer activity. This segment contains one large 97-base pair footprint, whose sequence has been conserved between the human and mouse genes. Binding sites for the liver-specific transcription factors HNF-1 and HNF-3 are present within this footprint.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
C.-C. Wu, J.-C. Lin, S.-C. Yang, C.-W. Lin, J. J.W. Chen, J.-Y. Shih, T.-M. Hong, and P.-C. Yang
Modulation of the expression of the invasion-suppressor CRMP-1 by cyclooxygenase-2 inhibition via reciprocal regulation of Sp1 and C/EBP{alpha}
Mol. Cancer Ther., June 1, 2008; 7(6): 1365 - 1375.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Xiong, W. E. Tapprich, and G. S. Cox
Mechanism of Gonadotropin Gene Expression. IDENTIFICATION OF A NOVEL NEGATIVE REGULATORY ELEMENT AT THE TRANSCRIPTION START SITE OF THE GLYCOPROTEIN HORMONE alpha -SUBUNIT GENE
J. Biol. Chem., October 18, 2002; 277(43): 40235 - 40246.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
H. Schrem, J. Klempnauer, and J. Borlak
Liver-Enriched Transcription Factors in Liver Function and Development. Part I: The Hepatocyte Nuclear Factor Network and Liver-Specific Gene Expression
Pharmacol. Rev., March 1, 2002; 54(1): 129 - 158.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. A. Hegele, H. Cao, S. B. Harris, A. J. G. Hanley, B. Zinman, and P. W. Connelly
The Private Hepatocyte Nuclear Factor-1{alpha} G319S Variant Is Associated With Plasma Lipoprotein Variation in Canadian Oji-Cree
Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 217 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
F. M. van 't Hooft, S. Jormsjö, B. Lundahl, P. Tornvall, P. Eriksson, and A. Hamsten
A functional polymorphism in the apolipoprotein B promoter that influences the level of plasma low density lipoprotein
J. Lipid Res., September 1, 1999; 40(9): 1686 - 1694.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
S. Goodison, K. Yoshida, M. Churchman, and D. Tarin
Multiple Intron Retention Occurs in Tumor Cell CD44 mRNA Processing
Am. J. Pathol., October 1, 1998; 153(4): 1221 - 1228.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yamashita, T. Yoshimasa, H. Arai, J. Hiraoka, K. Takaya, Y. Miyamoto, Y. Ogawa, H. Itoh, and K. Nakao
Identification of Cis-elements of the Human Endothelin-A Receptor Gene and Inhibition of the Gene Expression by the Decoy Strategy
J. Biol. Chem., June 26, 1998; 273(26): 15993 - 15999.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. D. Cooper, J. Chen, M. J. Botelho-Yetkinler, Y. Cao, T. Taniguchi, and B. Levy-Wilson
Characterization of Hepatic-specific Regulatory Elements in the Promoter Region of the Human Cholesterol 7alpha -Hydroxylase Gene
J. Biol. Chem., February 7, 1997; 272(6): 3444 - 3452.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D. Kardassis, M. Laccotripe, I. Talianidis, and V. Zannis
Transcriptional Regulation of the Genes Involved in Lipoprotein Transport : The Role of Proximal Promoters and Long-range Regulatory Elements and Factors in Apolipoprotein Gene Regulation
Hypertension, April 1, 1996; 27(4): 980 - 1008.
[Full Text]

Home page
 J. Biol. Chem.Home page
S.-Y. Lee, B. P. Nagy, A. R. Brooks, D.-M. Wang, B. Paulweber, and B. Levy-Wilson
Members of the Caudal Family of Homeodomain Proteins Repress Transcription from the Human Apolipoprotein B Promoter in Intestinal Cells
J. Biol. Chem., January 12, 1996; 271(2): 707 - 718.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. J. Antes, J. Chen, A. D. Cooper, and B. Levy-Wilson
The Nuclear Matrix Protein CDP Represses Hepatic Transcription of the Human Cholesterol-7alpha Hydroxylase Gene
J. Biol. Chem., August 18, 2000; 275(34): 26649 - 26660.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. J. Antes, S. A. Goodart, C. Huynh, M. Sullivan, S. G. Young, and B. Levy-Wilson
Identification and Characterization of a 315-Base Pair Enhancer, Located More than 55 Kilobases 5' of the Apolipoprotein B Gene, That Confers Expression in the Intestine
J. Biol. Chem., August 18, 2000; 275(34): 26637 - 26648.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-C. Wang, M. Waltner-Law, K. Yamada, H. Osawa, S. Stifani, and D. K. Granner
Transducin-like Enhancer of Split Proteins, the Human Homologs of Drosophila Groucho, Interact with Hepatic Nuclear Factor 3beta
J. Biol. Chem., June 9, 2000; 275(24): 18418 - 18423.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1991 by the American Society for Biochemistry and Molecular Biology.