JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arias, J. A.
Right arrow Articles by Dynan, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arias, J. A.
Right arrow Articles by Dynan, W. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 266, Issue 13, 8055-8061, 05, 1991

Promoter-dependent phosphorylation of RNA polymerase II by a template- bound kinase. Association with transcriptional initiation

JA Arias, SR Peterson and WS Dynan
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.

The largest subunit of eukaryotic RNA polymerase II (RNAP II) has a serine- and threonine-rich C-terminal domain (CTD) that may interact both with DNA and with the activating region of transcription factors. It has been proposed, in one model, that a protein kinase phosphorylates the promoter-associated CTD, facilitating the transition between promoter-binding and RNA-elongating forms of RNAP II. An immobilized template transcription system was used to test the predictions of this model directly. A protein kinase that phosphorylated the CTD at multiple sites was detected. This activity was tightly bound to the template, as evidenced by continued association after multiple rounds of washing. Phosphorylation was promoter sequence-dependent and exhibited the same nucleotide substrate specificity as the previously characterized ATP-requiring step in initiation. It was necessary for [gamma-32P]ATP and initiating rNTPs to be present simultaneously in the reaction in order to efficiently chase- radiolabel into elongating RNAP II-containing complexes, consistent with the idea that initiation and phosphorylation are temporally associated reactions.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
S. Vagner, C. Vagner, and I. W. Mattaj
The carboxyl terminus of vertebrate poly(A) polymerase interacts with U2AF 65 to couple 3'-end processing and splicing
Genes & Dev., February 15, 2000; 14(4): 403 - 413.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Trigon, H. Serizawa, J. W. Conaway, R. C. Conaway, S. P. Jackson, and M. Morange
Characterization of the Residues Phosphorylated in Vitro by Different C-terminal Domain Kinases
J. Biol. Chem., March 20, 1998; 273(12): 6769 - 6775.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. B. Cary, S. R. Peterson, J. Wang, D. G. Bear, E. M. Bradbury, and D. J. Chen
DNA looping by Ku and the DNA-dependent protein kinase
PNAS, April 29, 1997; 94(9): 4267 - 4272.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Fewell and E. Kuff
Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium
J. Cell Sci., January 7, 1996; 109(7): 1937 - 1946.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
L Zawel, K P Kumar, and D Reinberg
Recycling of the general transcription factors during RNA polymerase II transcription.
Genes & Dev., June 15, 1995; 9(12): 1479 - 1490.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. Trigon and M. Morange
Different Carboxyl-terminal Domain Kinase Activities Are Induced by Heat-shock and Arsenite
J. Biol. Chem., June 2, 1995; 270(22): 13091 - 13098.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Peterson, S. A. Jesch, T. N. Chamberlin, A. Dvir, S. K. Rabindran, C. Wu, and W. S. Dynan
Stimulation of the DNA-dependent Protein Kinase by RNA Polymerase II Transcriptional Activator Proteins
J. Biol. Chem., January 20, 1995; 270(3): 1449 - 1454.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A Kuhn, T M Gottlieb, S P Jackson, and I Grummt
DNA-dependent protein kinase: a potent inhibitor of transcription by RNA polymerase I.
Genes & Dev., January 15, 1995; 9(2): 193 - 203.
[Abstract] [PDF]

Home page
 ScienceHome page
L Schaeffer, R Roy, S Humbert, V Moncollin, W Vermeulen, J. Hoeijmakers, P Chambon, and J. Egly
DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor
Science, April 2, 1993; 260(5104): 58 - 63.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
D Herschlag and F B Johnson
Synergism in transcriptional activation: a kinetic view.
Genes & Dev., February 1, 1993; 7(2): 173 - 179.
[PDF]

Home page
 ScienceHome page
O Gileadi, W. Feaver, and R. Kornberg
Cloning of a subunit of yeast RNA polymerase II transcription factor b and CTD kinase
Science, September 4, 1992; 257(5075): 1389 - 1392.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
S R Peterson, A Dvir, C W Anderson, and W S Dynan
DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats.
Genes & Dev., March 1, 1992; 6(3): 426 - 438.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1991 by the American Society for Biochemistry and Molecular Biology.