| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 13, 8551-8559, May, 1991
M Becker-Andre, P Schulze-Lefert and K Hahlbrock
4-Coumarate:CoA ligase (4CL), a key enzyme of phenylpropanoid metabolism in
plants, is encoded in potato (Solanum tuberosum L.) by two structurally
similar genes (St4cl-1, St4cl-2). Computer-based sequence analyses revealed
similarities at the amino acid sequence level with other enzymes dependent
on ATP for activation of aromatic carboxylic acids, e.g. some bacterial
peptide synthetases. All these enzymes have a common seven amino acid
sequence motif containing one cysteine residue. Using an assay on the basis
of the polymerase chain reaction, we show that the mRNAs from both 4CL
genes accumulate to equal levels in suspension-cultured cells and whole
plant tissues, independent of various kinds of activating stimulus applied
and of the overall transcriptional activity of the genes. The apparent lack
of differential expression, together with the fact that both 4CL genes and
proteins are nearly identical in structure, make it unlikely that 4CL
isoforms in potato have specific roles in metabolic channeling.
Constitutive in vivo footprints in the TATA-box proximal region of the
St4cl-1 promoter define putative cis-acting elements which may be involved
in the responses of the 4CL genes to various endogenous and exogenous
stimuli.
Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate: CoA ligase genes in potato
Max-Planck-Institut fur Zuchtungsforschung, Abteilung Biochemie, Koln, Federal Republic of Germany.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Hamberger and K. Hahlbrock The 4-coumarate:CoA ligase gene family in Arabidopsis thaliana comprises one rare, sinapate-activating and three commonly occurring isoenzymes PNAS, February 17, 2004; 101(7): 2209 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dai, Z. Zhang, S. Chen, and R. N. Beachy RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease PNAS, January 13, 2004; 101(2): 687 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pietrowska-Borek, H.-P. Stuible, E. Kombrink, and A. Guranowski 4-Coumarate:Coenzyme A Ligase Has the Catalytic Capacity to Synthesize and Reuse Various (Di)Adenosine Polyphosphates Plant Physiology, March 1, 2003; 131(3): 1401 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lindermayr, J. Fliegmann, and J. Ebel Deletion of a Single Amino Acid Residue from Different 4-Coumarate:CoA Ligases from Soybean Results in the Generation of New Substrate Specificities J. Biol. Chem., January 24, 2003; 278(5): 2781 - 2786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaneko, Y. Ohnishi, and S. Horinouchi Cinnamate:Coenzyme A Ligase from the Filamentous Bacterium Streptomyces coelicolor A3(2) J. Bacteriol., January 1, 2003; 185(1): 20 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Harding, J. Leshkevich, V. L. Chiang, and C.-J. Tsai Differential Substrate Inhibition Couples Kinetically Distinct 4-Coumarate:Coenzyme A Ligases with Spatially Distinct Metabolic Roles in Quaking Aspen Plant Physiology, February 1, 2002; 128(2): 428 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schmidt, R. Grimm, J. Schmidt, D. Scheel, D. Strack, and S. Rosahl Cloning and Expression of a Potato cDNA Encoding Hydroxycinnamoyl-CoA:Tyramine N-(Hydroxycinnamoyl)transferase J. Biol. Chem., February 12, 1999; 274(7): 4273 - 4280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Hu, A. Kawaoka, C.-J. Tsai, J. Lung, K. Osakabe, H. Ebinuma, and V. L. Chiang Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides) PNAS, April 28, 1998; 95(9): 5407 - 5412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Allina, A. Pri-Hadash, D. A. Theilmann, B. E. Ellis, and C. J. Douglas 4-Coumarate:Coenzyme A Ligase in Hybrid Poplar . Properties of Native Enzymes, cDNA Cloning, and Analysis of Recombinant Enzymes Plant Physiology, February 1, 1998; 116(2): 743 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Minambres, H. Martinez-Blanco, E. R. Olivera, B. Garcia, B. Diez, J. L. Barredo, M. A. Moreno, C. Schleissner, F. Salto, and J. M. Luengo Molecular Cloning and Expression in Different Microbes of the DNA Encoding Pseudomonas putida U Phenylacetyl-CoA Ligase. USE OF THIS GENE TO IMPROVE THE RATE OF BENZYLPENICILLIN BIOSYNTHESIS IN PENICILLIUM CHRYSOGENUM J. Biol. Chem., December 27, 1996; 271(52): 33531 - 33538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Steffensky, S.-M. Li, and L. Heide Cloning, Overexpression, and Purification of Novobiocic Acid Synthetase from Streptomyces spheroides NCIMB 11891 J. Biol. Chem., July 7, 2000; 275(28): 21754 - 21760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gobel, I. Feussner, A. Schmidt, D. Scheel, J. Sanchez-Serrano, M. Hamberg, and S. Rosahl Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells J. Biol. Chem., February 23, 2001; 276(9): 6267 - 6273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Stuible and E. Kombrink Identification of the Substrate Specificity-conferring Amino Acid Residues of 4-Coumarate:Coenzyme A Ligase Allows the Rational Design of Mutant Enzymes with New Catalytic Properties J. Biol. Chem., July 13, 2001; 276(29): 26893 - 26897. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
