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J. Biol. Chem., Vol. 266, Issue 15, 9586-9590, 05, 1991

Bovine mitochondrial ribosomes possess a high affinity binding site for guanine nucleotides

ND Denslow, JC Anders and TW O'Brien
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.

Mammalian mitochondrial ribosomes possess a binding site for guanine nucleotides. GTP binds in unit stoichiometry and with high affinity (Kd = 15.3 +/- 2.8 nM) to the small subunit of bovine mitochondrial ribosomes. This binding activity survives high salt washes, indicating that the nucleotide binds to an integral site within this subunit. GDP also binds to the small subunit with high affinity (Kd = 17 +/- 5.8 nm) and in unit stoichiometry. The GTP binding activity can be competed with GDP but not appreciably by other nucleotides, indicating that both GTP and GDP bind specifically and to the same site. The non- hydrolyzable analogs of GTP, guanylyl-5'-imidophosphate, and guanylyl- (beta,gamma-methylene)- diphosphonate also bind to the small subunit, but with reduced affinity. These results indicate that mammalian mitochondrial ribosomes, unlike other ribosomes, are able to interact directly with guanosine triphosphate, suggesting that the bound GTP may be involved in a novel regulatory mechanism in mitochondrial protein synthesis.
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