| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 16, 10131-10135, Jun, 1991
NG Anderson, E Kilgour and TW Sturgill
Treatment of BC3H1 myocytes or 3T3-L1 fibroblasts with fluoroaluminate
(AlF4-), a direct activator of G proteins, increased the tyrosine
phosphorylation of a 42-kDa cytosolic protein. AlF4- induced a parallel
increase in protein kinase activity toward myelin basic protein (MBP) in
partially purified cell extracts. To test whether AlF4- was activating the
42-kDa MAP (mitogen-activated protein) kinase, extracts from AlF4--treated
cells were taken through the chromatographic steps routinely used to purify
MAP kinase from growth factor-stimulated cells. Following phenyl-Superose
chromatography, a peak of MBP kinase activity eluted at a position
characteristic of MAP kinase. Immunoblotting of the active fractions with
anti-phosphotyrosine antibodies revealed a single reactive protein band of
Mr 42,000. Stimulation of MAP kinase by AlF4- was rapid, peaking within 15
min and persisting for at least 1 h. In contrast, the activation of MAP
kinase by insulin was transient, characteristic of its activation by growth
factors in other cell types. Although concentrations of sodium fluoride
greater than 1 mM also activated MAP kinase, this effect was shown to be
dependent upon the simultaneous presence of aluminum ions in the medium.
Activation of MAP kinase by AlF4- was not affected by either cellular
depletion of protein kinase C or pretreatment of cells with pertussis
toxin. Potential sites of action of AlF4- are discussed. These findings
suggest that activation of a G protein(s) in intact cells can initiate
events that result in tyrosine phosphorylation and activation of MAP
kinase.
Activation of mitogen-activated protein kinase in BC3H1 myocytes by fluoroaluminate
Department of Internal Medicine, University of Virginia, Charlottesville 22908.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Zhande, W. Zhang, Y. Zheng, E. Pendleton, Y. Li, R. D. Polakiewicz, and X. J. Sun Dephosphorylation by Default, a Potential Mechanism for Regulation of Insulin Receptor Substrate-1/2, Akt, and ERK1/2 J. Biol. Chem., December 22, 2006; 281(51): 39071 - 39080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. V. Bogatcheva, P. Wang, A. A. Birukova, A. D. Verin, and J. G. N. Garcia Mechanism of fluoride-induced MAP kinase activation in pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1139 - L1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Jacobs, K. A. Boldingh, H. H. Slagsvold, G. H. Thoresen, and R. E. Paulsen ERK2 Prohibits Apoptosis-induced Subcellular Translocation of Orphan Nuclear Receptor NGFI-B/TR3 J. Biol. Chem., November 26, 2004; 279(48): 50097 - 50101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li THE BIOCHEMISTRYAND PHYSIOLOGYOF METALLIC FLUORIDE: ACTION, MECHANISM, AND IMPLICATIONS Crit. Rev. Oral. Biol. Med., March 1, 2003; 14(2): 100 - 114. [Abstract] [Full Text]
|
|
|
|
|
|
W. Chen, M. A. White, and M. H. Cobb Stimulus-specific Requirements for MAP3 Kinases in Activating the JNK Pathway J. Biol. Chem., December 13, 2002; 277(51): 49105 - 49110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. O. Andersen, M. Enger, G. H. Thoresen, T. Skomedal, and J.-B. Osnes alpha 1-Adrenergic activation of myocardial Na-K-2Cl cotransport involving mitogen-activated protein kinase Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H641 - H652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Horgan and P. F. Copenhaver G Protein-Mediated Inhibition of Neuronal Migration Requires Calcium Influx J. Neurosci., June 1, 1998; 18(11): 4189 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Michael, K. C. Martin, R. Seger, M.-M. Ning, R. Baston, and E. R. Kandel Repeated pulses of serotonin required for long-term facilitation activate mitogen-activated protein kinase in sensory neurons of Aplysia PNAS, February 17, 1998; 95(4): 1864 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ohmichi, K. Koike, A. Kimura, K. Masuhara, H. Ikegami, Y. Ikebuchi, T. Kanzaki, K. Touhara, M. Sakaue, Y. Kobayashi, et al. Role of Mitogen-Activated Protein Kinase Pathway in Prostaglandin F2{alpha}-Induced Rat Puerperal Uterine Contraction Endocrinology, August 1, 1997; 138(8): 3103 - 3111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-W. Wu, H. K. Yoon, D. J. Baylink, L. M. Graves, and K.-H. W. Lau Fluoride at Mitogenic Doses Induces a Sustained Activation of p44mapk, but Not p42mapk, in Human TE85 Osteosarcoma Cells J. Clin. Endocrinol. Metab., April 1, 1997; 82(4): 1126 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Shapiro, J. N. Evans, R. J. Davis, and J. A. Posada The Seven-transmembrane-spanning Receptors for Endothelin and Thrombin Cause Proliferation of Airway Smooth Muscle Cells and Activation of the Extracellular Regulated Kinase and c-Jun NH(2)-terminal Kinase Groups of Mitogen-activated Protein Kinases J. Biol. Chem., March 8, 1996; 271(10): 5750 - 5754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Clark and K. J. Clark Evidence That the Bradykinin-induced Activation of Phospholipase D and of the Mitogen-activated Protein Kinase Cascade Involve Different Protein Kinase C Isoforms J. Biol. Chem., March 31, 1995; 270(13): 7097 - 7103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Horgan, M. Lagrange, and P. Copenhaver Developmental expression of G proteins in a migratory population of embryonic neurons Development, January 4, 1994; 120(4): 729 - 742. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
