| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 17, 10768-10774, 06, 1991
DA Willins, CW Ryan, JV Platko and JM Calvo
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Exogenous leucine affects the expression of a number of different operons in Escherichia coli. For at least some of these operons, the leucine-related effect is mediated by a protein called Lrp (Leucine- responsive regulatory protein). The purification of Lrp to near homogeneity is described. Lrp is a moderately abundant, basic protein composed of two subunits of molecular mass 18.8 kDa each. In addition, the corresponding protein was purified from a strain having a mutation within the gene that encodes Lrp (lrp). This mutation (lrp-1) causes high constitutive expression of ilvIH, one of the operons controlled by Lrp (Platko, J. V., Willins, D.A., and Calvo, J.M. (1990) J. Bacteriol. 172, 4563-4570). The Lrp-1 and Lrp proteins have similar physical properties, but they show some differences in the characteristics with which they bind DNA upstream of the ilvIH promoter. The nucleotide sequences of the lrp and lrp-1 genes differ by only a single nucleotide, a C to G change that would substitute a Glu for an Asp at amino acid 114. Lrp has some amino acid sequence similarity to AsnC, a protein that regulates asnA expression (Kolling, R., and Lother, H. (1985) J. Bacteriol. 164, 310-315).
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Kumarevel, N. Nakano, K. Ponnuraj, S. C. B. Gopinath, K. Sakamoto, A. Shinkai, P. K. R. Kumar, and S. Yokoyama Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding Nucleic Acids Res., July 24, 2008; (2008) gkn456v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. McFarland, S. Lucchini, J. C. D. Hinton, and C. J. Dorman The Leucine-Responsive Regulatory Protein, Lrp, Activates Transcription of the fim Operon in Salmonella enterica Serovar Typhimurium via the fimZ Regulatory Gene J. Bacteriol., January 15, 2008; 190(2): 602 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shrivastava and R. Ramachandran Mechanistic insights from the crystal structures of a feast/famine regulatory protein from Mycobacterium tuberculosis H37Rv Nucleic Acids Res., December 18, 2007; 35(21): 7324 - 7335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-R. Lee, S.-H. Cho, M.-J. Yoon, A. Peterkofsky, and Y.-J. Seok Escherichia coli enzyme IIANtr regulates the K+ transporter TrkA PNAS, March 6, 2007; 104(10): 4124 - 4129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Thaw, S. E. Sedelnikova, T. Muranova, S. Wiese, S. Ayora, J. C. Alonso, A. B. Brinkman, J. Akerboom, J. van der Oost, and J. B. Rafferty Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family Nucleic Acids Res., March 9, 2006; 34(5): 1439 - 1449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cordone, E. M. F. Mauriello, D. J. Pickard, G. Dougan, M. De Felice, and E. Ricca The lrp Gene and Its Role in Type I Fimbriation in Citrobacter rodentium J. Bacteriol., October 15, 2005; 187(20): 7009 - 7017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lahooti, P. L. Roesch, and I. C. Blomfield Modulation of the Sensitivity of FimB Recombination to Branched-Chain Amino Acids and Alanine in Escherichia coli K-12 J. Bacteriol., September 15, 2005; 187(18): 6273 - 6280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Oram, A. Avdalovic, and R. K. Holmes Analysis of Genes That Encode DtxR-Like Transcriptional Regulators in Pathogenic and Saprophytic Corynebacterial Species Infect. Immun., April 1, 2004; 72(4): 1885 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lodwig, S. Kumar, D. Allaway, A. Bourdes, J. Prell, U. Priefer, and P. Poole Regulation of L-Alanine Dehydrogenase in Rhizobium leguminosarum bv. viciae and Its Role in Pea Nodules J. Bacteriol., February 1, 2004; 186(3): 842 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jafri, S. Chen, and J. M. Calvo ilvIH Operon Expression in Escherichia coli Requires Lrp Binding to Two Distinct Regions of DNA J. Bacteriol., October 1, 2002; 184(19): 5293 - 5300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Friedberg, M. Midkiff, and J. M. Calvo Global versus Local Regulatory Roles for Lrp-Related Proteins: Haemophilus influenzae as a Case Study J. Bacteriol., July 1, 2001; 183(13): 4004 - 4011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Beloin, R. Exley, A.-L. Mahé, M. Zouine, S. Cubasch, and F. Le Hégarat Characterization of LrpC DNA-Binding Properties and Regulation of Bacillus subtilis lrpC Gene Expression J. Bacteriol., August 15, 2000; 182(16): 4414 - 4424. [Abstract] [Full Text]
|
|
|
|
|
|
E. R. Zinser and R. Kolter Prolonged Stationary-Phase Incubation Selects for lrp Mutations in Escherichia coli K-12 J. Bacteriol., August 1, 2000; 182(15): 4361 - 4365. [Abstract] [Full Text]
|
|
|
|
|
|
J. Enoru-Eta, D. Gigot, T.-L. Thia-Toong, N. Glansdorff, and D. Charlier Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp J. Bacteriol., July 1, 2000; 182(13): 3661 - 3672. [Abstract] [Full Text]
|
|
|
|
|
|
A. Tapias, G. Lopez, and S. Ayora Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein which bridges DNA Nucleic Acids Res., January 15, 2000; 28(2): 552 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ali Azam, A. Iwata, A. Nishimura, S. Ueda, and A. Ishihama Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid J. Bacteriol., October 15, 1999; 181(20): 6361 - 6370. [Abstract] [Full Text]
|
|
|
|
|
|
K. T. Madhusudhan, J. Luo, and J. R. Sokatch In Vitro Transcriptional Studies of the bkd Operon of Pseudomonas putida: L-Branched-Chain Amino Acids and D-Leucine Are the Inducers J. Bacteriol., May 1, 1999; 181(9): 2889 - 2894. [Abstract] [Full Text]
|
|
|
|
|
|
A. Napoli, J. van der Oost, C. W. Sensen, R. L. Charlebois, M. Rossi, and M. Ciaramella An Lrp-Like Protein of the Hyperthermophilic Archaeon Sulfolobus solfataricus Which Binds to Its Own Promoter J. Bacteriol., March 1, 1999; 181(5): 1474 - 1480. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Y. Rhee, B. S. Parekh, and G. W. Hatfield Leucine-responsive Regulatory Protein-DNA Interactions in the Leader Region of the ilvGMEDA Operon of Escherichia coli J. Biol. Chem., October 25, 1996; 271(43): 26499 - 26507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cui, M. A. Midkiff, Q. Wang, and J. M. Calvo The Leucine-responsive Regulatory Protein (Lrp) from Escherichia coli J. Biol. Chem., March 22, 1996; 271(12): 6611 - 6617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brevet, J. Chen, F. Lévque, S. Blanquet, and P. Plateau Comparison of the Enzymatic Properties of the Two Escherichia coli Lysyl-tRNA Synthetase Species J. Biol. Chem., June 16, 1995; 270(24): 14439 - 14444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Brinkman, I. Dahlke, J. E. Tuininga, T. Lammers, V. Dumay, E. de Heus, J. H. G. Lebbink, M. Thomm, W. M. de Vos, and J. van der Oost An Lrp-like Transcriptional Regulator from the Archaeon Pyrococcus furiosus Is Negatively Autoregulated J. Biol. Chem., December 1, 2000; 275(49): 38160 - 38169. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
