HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Amano, J. Articles by Kobata, A. Search for Related Content PubMed PubMed Citation Articles by Amano, J. Articles by Kobata, A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 18, 11461-11477, 06, 1991

Structures of mucin-type sugar chains of the galactosyltransferase purified from human milk. Occurrence of the ABO and Lewis blood group determinants

J Amano, P Straehl, EG Berger, N Kochibe and A Kobata
Department of Biochemistry, University of Tokyo, Japan.

The mucin-type sugar chains of human milk galactosyltransferase samples purified from two donors with different blood types were released by alkaline borohydride treatment and quantitatively labeled by N- [3H]acetylation. The radioactive oligosaccharides thus obtained were fractionated by high performance liquid chromatography and immobilized lectin chromatography, and their structures were studied by sequential digestion with endo- or exoglycosidases, methylation analysis, and periodate oxidation. It was revealed that the structures of the mucin- type sugar chains of galactosyltransferase are extremely various, and many blood group determinants are expressed on more than 13 different backbone sugar chains. The characteristic features of the sugar chains could be summarized as follows. 1) The sugar chains of both samples are composed of core 1, Gal beta 1----3GalNAc, and core 2, GlcNAc beta 1---- 6(Gal beta 1----3)GalNAc. 2) One or two N-acetyllactosamine repeating units extend from the core through GlcNAc beta 1----6Gal and GlcNAc beta 1----3 Gal linkages. 3) Blood group determinants are expressed in accord with the blood types of the donors: sample 1 from a donor of blood type O, Lea+b- contains oligosaccharides with Lea and X determinants, and sample 2 from a donor of B, Lea-b- contains those with H, X, Y, and B determinants.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. J. Olson, M. Backstrom, H. Karlsson, J. Burchell, and G. C. Hansson A MUC1 tandem repeat reporter protein produced in CHO-K1 cells has sialylated core 1 O-glycans and becomes more densely glycosylated if coexpressed with polypeptide-GalNAc-T4 transferase Glycobiology, February 1, 2005; 15(2): 177 - 191. [Abstract] [Full Text] [PDF]

				J. Amano and M. Oshima Expression of the H Type 1 Blood Group Antigen during Enterocytic Differentiation of Caco-2 Cells J. Biol. Chem., July 23, 1999; 274(30): 21209 - 21216. [Abstract] [Full Text] [PDF]

				I. Pastuszak, C. Ketchum, G. Hermanson, E. J. Sjoberg, R. Drake, and A. D. Elbein GDP-L-fucose Pyrophosphorylase. PURIFICATION, cDNA CLONING, AND PROPERTIES OF THE ENZYME J. Biol. Chem., November 13, 1998; 273(46): 30165 - 30174. [Abstract] [Full Text] [PDF]