| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 19, 12162-12167, Jul, 1991
LM Carruth, S Demczuk and SB Mizel
Department of Microbiology and Immunology, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
Interleukin (IL) 1 alpha is synthesized as a 33-kDa precursor that is enzymatically cleaved to the 15-17-kDa forms that are found in the culture supernatants of activated macrophages. We have explored the possibility that calcium might enhance IL-1 processing and secretion via the stimulation of a calcium-dependent protease. We have found that lysates prepared from human peripheral blood monocytes, the human histiocytic lymphoma cell line U937, and the murine macrophage cell line P388D1 contain a calcium-dependent IL-1 alpha processing activity that cleaves the IL-1 alpha precursor to its mature form. Although NIH 3T3 mouse fibroblast cell lysates also contain IL-1 processing activity, lysates from the murine thymoma EL-4, the human epidermoid cell line HEp-2, and the human foreskin fibroblast line FS-4 lack this activity. IL-1 processing activity is inhibited by leupeptin and exhibits a molecular mass of 80-110 kDa. The processing activity is also inhibited by a monoclonal antibody directed against calpain type I. These results indicate that the processing of the IL-1 alpha precursor is mediated, at least in part, by a member of the calpain family of proteases. Mixing experiments revealed that lysates from EL-4 or HEp-2 cells contain an inhibitor(s) of the calpain-like protease in macrophage extracts. It is, therefore, likely that many non-macrophage cell types are unable to process the IL-1 alpha precursor because the calpain present in these cells is only weakly active due to the presence of a specific inhibitor(s) such as calpastatin.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Pelegrin, C. Barroso-Gutierrez, and A. Surprenant P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1{beta} in Mouse Macrophage J. Immunol., June 1, 2008; 180(11): 7147 - 7157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schultz, V. Murthy, J. B. Tatro, and D. Beasley Endogenous interleukin-1{alpha} promotes a proliferative and proinflammatory phenotype in human vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2927 - H2934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Niki, H. Yamada, T. Kikuchi, Y. Toyama, H. Matsumoto, K. Fujikawa, and N. Tada Membrane-Associated IL-1 Contributes to Chronic Synovitis and Cartilage Destruction in Human IL-1{alpha} Transgenic Mice J. Immunol., January 1, 2004; 172(1): 577 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kumar, H. Kishimoto, H. L. Chua, S. Badve, K. D. Miller, R. M. Bigsby, and H. Nakshatri Interleukin-1{alpha} Promotes Tumor Growth and Cachexia in MCF-7 Xenograft Model of Breast Cancer Am. J. Pathol., December 1, 2003; 163(6): 2531 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Brough, R. A. Le Feuvre, R. D. Wheeler, N. Solovyova, S. Hilfiker, N. J. Rothwell, and A. Verkhratsky Ca2+ Stores and Ca2+ Entry Differentially Contribute to the Release of IL-1{beta} and IL-1{alpha} from Murine Macrophages J. Immunol., March 15, 2003; 170(6): 3029 - 3036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Perregaux, K. Bhavsar, L. Contillo, J. Shi, and C. A. Gabel Antimicrobial Peptides Initiate IL-1{beta} Posttranslational Processing: A Novel Role Beyond Innate Immunity J. Immunol., March 15, 2002; 168(6): 3024 - 3032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Perregaux, P. McNiff, R. Laliberte, N. Hawryluk, H. Peurano, E. Stam, J. Eggler, R. Griffiths, M. A. Dombroski, and C. A. Gabel Identification and Characterization of a Novel Class of Interleukin-1 Post-Translational Processing Inhibitors J. Pharmacol. Exp. Ther., October 1, 2001; 299(1): 187 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Perregaux, P. McNiff, R. Laliberte, M. Conklyn, and C. A. Gabel ATP Acts as an Agonist to Promote Stimulus-Induced Secretion of IL-1{beta} and IL-18 in Human Blood J. Immunol., October 15, 2000; 165(8): 4615 - 4623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shi, V. Y. Melnikov, R. W. Schrier, and C. L. Edelstein Downregulation of the calpain inhibitor protein calpastatin by caspases during renal ischemia-reperfusion Am J Physiol Renal Physiol, September 1, 2000; 279(3): F509 - F517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Laliberte, J. Eggler, and C. A. Gabel ATP Treatment of Human Monocytes Promotes Caspase-1 Maturation and Externalization J. Biol. Chem., December 24, 1999; 274(52): 36944 - 36951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. EDELSTEIN, Y. SHI, and R. W. SCHRIER Role of Caspases in Hypoxia-Induced Necrosis of Rat Renal Proximal Tubules J. Am. Soc. Nephrol., September 1, 1999; 10(9): 1940 - 1949. [Abstract] [Full Text]
|
|
|
|
|
|
D. Beasley and A. L. Cooper Constitutive expression of interleukin-1alpha precursor promotes human vascular smooth muscle cell proliferation Am J Physiol Heart Circ Physiol, March 1, 1999; 276(3): H901 - H912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Perregaux and C. A. Gabel Human monocyte stimulus-coupled IL-1beta posttranslational processing: modulation via monovalent cations Am J Physiol Cell Physiol, December 1, 1998; 275(6): C1538 - C1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Perregaux and C. A. Gabel Post-Translational Processing of Murine IL-1: Evidence that ATP-Induced Release of IL-1{alpha} and IL-1{beta} Occurs via a Similar Mechanism J. Immunol., March 1, 1998; 160(5): 2469 - 2477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Hill, G. Kwon, C. A. Marshall, and M. L. McDaniel Hyperglycemic Levels of Glucose Inhibit Interleukin 1 Release from RAW 264.7 Murine Macrophages by Activation of Protein Kinase C J. Biol. Chem., February 6, 1998; 273(6): 3308 - 3313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. McMahon, S. Garfinkel, I. Prudovsky, X. Hu, and T. Maciag Intracellular Precursor Interleukin (IL)-1alpha , but Not Mature IL-1alpha , Is Able to Regulate Human Endothelial Cell Migration in Vitro J. Biol. Chem., November 7, 1997; 272(45): 28202 - 28205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Hill, J. A. Corbett, G. Kwon, C. A. Marshall, and M. L. McDaniel Nitric Oxide Regulates Interleukin 1Bioactivity Released from Murine Macrophages J. Biol. Chem., September 13, 1996; 271(37): 22672 - 22678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Kavita and S. B. Mizel Differential Sensitivity of Interleukin-1alpha and -beta Precursor Proteins to Cleavage by Calpain, a Calcium-dependent Protease J. Biol. Chem., November 17, 1995; 270(46): 27758 - 27765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Siders and S. B. Mizel Interleukin-1[IMAGE] Secretion J. Biol. Chem., July 7, 1995; 270(27): 16258 - 16264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Kuida, J. Lippke, G Ku, M. Harding, D. Livingston, M. Su, and R. Flavell Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme Science, March 31, 1995; 267(5206): 2000 - 2003. [Abstract] [PDF]
|
|
|
|
|
|
R. V. Deshpande, J.-M. Goust, A. K. Chakrabarti, E. Barbosa, E. L. Hogan, and N. L. Banik Calpain Expression in Lymphoid Cells J. Biol. Chem., February 10, 1995; 270(6): 2497 - 2505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Tarantini, I. Micucci, S. Bellum, M. Landriscina, S. Garfinkel, I. Prudovsky, and T. Maciag The Precursor but Not the Mature Form of IL1alpha Blocks the Release of FGF1 in Response to Heat Shock J. Biol. Chem., February 9, 2001; 276(7): 5147 - 5151. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
