HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Burdett, K. Articles by Hajra, A. K. Search for Related Content PubMed PubMed Citation Articles by Burdett, K. Articles by Hajra, A. K. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 19, 12201-12206, Jul, 1991

Peroxisomal localization of acyl-coenzyme A reductase (long chain alcohol forming) in guinea pig intestine mucosal cells

K Burdett, LK Larkins, AK Das and AK Hajra
Neuroscience Laboratory, University of Michigan, Ann Arbor 48104-1687.

Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which contained mainly mitochondria, lysosomes, and peroxisomes. Peroxisomes (marker enzymes: catalase and dihydroxyacetone phosphate acyltransferase) present in the L fraction were separated from other organelles in a Nycodenz density gradient centrifugation employing a vertical rotor. By comparing the distribution of acyl-CoA reductase with different marker enzymes in the gradient, it was concluded that this reductase is primarily localized in the microperoxisomes (microbodies). The topography of the membrane-bound enzyme in the isolated organelles was studied by checking its lability toward trypsin in the absence and presence of the detergent Triton X-100. The results suggested that acyl-CoA reductase is localized on the outer surface (cytosolic side) of microperoxisomal membrane.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K.'i. Moto, T. Yoshiga, M. Yamamoto, S. Takahashi, K. Okano, T. Ando, T. Nakata, and S. Matsumoto Pheromone gland-specific fatty-acyl reductase of the silkmoth, Bombyx mori PNAS, August 5, 2003; 100(16): 9156 - 9161. [Abstract] [Full Text] [PDF]

				A. K. Hajra, L. K. Larkins, A. K. Das, N. Hemati, R. L. Erickson, and O. A. MacDougald Induction of the Peroxisomal Glycerolipid-synthesizing Enzymes during Differentiation of 3T3-L1 Adipocytes. ROLE IN TRIACYLGLYCEROL SYNTHESIS J. Biol. Chem., March 24, 2000; 275(13): 9441 - 9446. [Abstract] [Full Text] [PDF]

				A. K. Das, M. D. Uhler, and A. K. Hajra Molecular Cloning and Expression of Mammalian Peroxisomal trans-2-Enoyl-coenzyme A Reductase cDNAs J. Biol. Chem., August 4, 2000; 275(32): 24333 - 24340. [Abstract] [Full Text] [PDF]