| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 2, 740-746, Jan, 1991
A Leyte, HB van Schijndel, C Niehrs, WB Huttner, MP Verbeet, K Mertens and JA van Mourik
The acidic region of the Factor VIII light chain was studied with regard to
structural requirements for the formation of a functional von Willebrand
factor (vWF)-binding site. Factor VIII mutants lacking the B domain, with
additional deletions and an amino acid replacement within the sequence
1649-1689 were constructed using site-directed mutagenesis and expressed in
Cos-1 cells. These mutants, which were recovered as single-chain molecules
with similar specific activities, were compared in their binding to
immobilized vWF. Deletion of amino acids 741-1648 or 741-1668 did not
affect the binding of Factor VIII to vWF. However, a mutant with a deletion
of residues 741-1689 was no longer capable of interacting with vWF. This
indicates a role for residues within the sequence 1669-1689 in the
formation of a vWF-binding site. When recombinant Factor VIII was expressed
in the presence of chlorate, an inhibitor of protein sulfation, the
resulting Factor VIII displayed strongly reduced binding to vWF. vWF
binding was completely abolished when within the sequence 1669-1689 the
tyrosine residue Tyr1680, which is part of a consensus tyrosine sulfation
sequence, was replaced by phenylalanine. The Factor VIII sequence 1673-1689
was identified as a high affinity substrate for tyrosylprotein
sulfotransferase (Km = 57 microM) in cell-free sulfation studies. It is
concluded that sulfation of Tyr1680 is required for the interaction of
Factor VIII with vWF. Two synthetic peptides that represent the sequence
1673-1689, but differ with respect to sulfation of Tyr1680 are shown to
have vWF binding affinity that is considerably lower than the Factor VIII
protein. Several models to accommodate our findings are discussed.
Sulfation of Tyr1680 of human blood coagulation factor VIII is essential for the interaction of factor VIII with von Willebrand factor
Department of Blood Coagulation, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Surmann-Schmitt, U. Dietz, T. Kireva, N. Adam, J. Park, A. Tagariello, P. Onnerfjord, D. Heinegard, U. Schlotzer-Schrehardt, R. Deutzmann, et al. Ucma, a Novel Secreted Cartilage-specific Protein with Implications in Osteogenesis J. Biol. Chem., March 14, 2008; 283(11): 7082 - 7093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sasaki, Y. Hosoda, A. Nagata, M. Ding, J.-M. Cheng, T. Miyamoto, S. Okano, A. Asano, I. Miyoshi, and T. Agui A Mutation in Tpst2 Encoding Tyrosylprotein Sulfotransferase Causes Dwarfism Associated with Hypothyroidism Mol. Endocrinol., July 1, 2007; 21(7): 1713 - 1721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Hoffhines, E. Damoc, K. G. Bridges, J. A. Leary, and K. L. Moore Detection and Purification of Tyrosine-sulfated Proteins Using a Novel Anti-sulfotyrosine Monoclonal Antibody J. Biol. Chem., December 8, 2006; 281(49): 37877 - 37887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bonomi, M. Busnelli, L. Persani, G. Vassart, and S. Costagliola Structural Differences in the Hinge Region of the Glycoprotein Hormone Receptors: Evidence from the Sulfated Tyrosine Residues Mol. Endocrinol., December 1, 2006; 20(12): 3351 - 3363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Onnerfjord, T. F. Heathfield, and D. Heinegard Identification of Tyrosine Sulfation in Extracellular Leucine-rich Repeat Proteins Using Mass Spectrometry J. Biol. Chem., January 2, 2004; 279(1): 26 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Pilloff and T. S. Leyh Allosteric and Catalytic Functions of the PPi-binding Motif in the ATP Sulfurylase-GTPase System J. Biol. Chem., December 12, 2003; 278(50): 50435 - 50441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Moore The Biology and Enzymology of Protein Tyrosine O-Sulfation J. Biol. Chem., June 27, 2003; 278(27): 24243 - 24246. [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Strott Sulfonation and Molecular Action Endocr. Rev., October 1, 2002; 23(5): 703 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Tait, J.-F. Dong, J. A. Lopez, I. W. Dawes, and B. H. Chong Site-directed mutagenesis of platelet glycoprotein Ibalpha demonstrating residues involved in the sulfation of tyrosines 276, 278, and 279 Blood, May 29, 2002; 99(12): 4422 - 4427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Fong, S. M. Alam, T. Imai, B. Haribabu, and D. D. Patel CX3CR1 Tyrosine Sulfation Enhances Fractalkine-induced Cell Adhesion J. Biol. Chem., May 24, 2002; 277(22): 19418 - 19423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D J Bowen Haemophilia A and haemophilia B: molecular insights Mol. Pathol., April 1, 2002; 55(2): 127 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Stoilova-McPhie, B. O. Villoutreix, K. Mertens, G. Kemball-Cook, and A. Holzenburg 3-Dimensional structure of membrane-bound coagulation factor VIII: modeling of the factor VIII heterodimer within a 3-dimensional density map derived by electron crystallography Blood, February 15, 2002; 99(4): 1215 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D J Bowen Haemophilia A and haemophilia B: molecular insights Mol. Pathol., February 1, 2002; 55(1): 1 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. N. van den Brink, E. A. M. Turenhout, N. Bovenschen, B. G. A. D. H. Heijnen, K. Mertens, M. Peters, and J. Voorberg Multiple VH genes are used to assemble human antibodies directed toward the A3-C1 domains of factor VIII Blood, February 15, 2001; 97(4): 966 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Preobrazhensky, S. Dragan, T. Kawano, M. A. Gavrilin, I. V. Gulina, L. Chakravarty, and P. E. Kolattukudy Monocyte Chemotactic Protein-1 Receptor CCR2B Is a Glycoprotein That Has Tyrosine Sulfation in a Conserved Extracellular N-Terminal Region J. Immunol., November 1, 2000; 165(9): 5295 - 5303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jacquemin, R. Lavend'homme, A. Benhida, B. Vanzieleghem, R. d'Oiron, J.-M. Lavergne, H. H. Brackmann, R. Schwaab, T. VandenDriessche, M. K. L. Chuah, et al. A novel cause of mild/moderate hemophilia A: mutations scattered in the factor VIII C1 domain reduce factor VIII binding to von Willebrand factor Blood, August 1, 2000; 96(3): 958 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jorieux, E. Fressinaud, J. Goudemand, C. Gaucher, D. Meyer, C. Mazurier, and the INSERM Network on Molecular Abnormalities in v Conformational changes in the D' domain of von Willebrand factor induced by CYS 25 and CYS 95 mutations lead to factor VIII binding defect and multimeric impairment Blood, May 15, 2000; 95(10): 3139 - 3145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Lenting, J. A. van Mourik, and K. Mertens The Life Cycle of Coagulation Factor VIII in View of Its Structure and Function Blood, December 1, 1998; 92(11): 3983 - 3996. [Full Text] [PDF]
|
|
|
|
|
|
J. F. Healey, R. T. Barrow, H. M. Tamim, I. M. Lubin, M. Shima, D. Scandella, and P. Lollar Residues Glu2181-Val2243 Contain a Major Determinant of the Inhibitory Epitope in the C2 Domain of Human Factor VIII Blood, November 15, 1998; 92(10): 3701 - 3709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-B. Ouyang and K. L. Moore Molecular Cloning and Expression of Human and Mouse Tyrosylprotein Sulfotransferase-2 and a Tyrosylprotein Sulfotransferase Homologue in Caenorhabditis elegans J. Biol. Chem., September 18, 1998; 273(38): 24770 - 24774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Beisswanger, D. Corbeil, C. Vannier, C. Thiele, U. Dohrmann, R. Kellner, K. Ashman, C. Niehrs, and W. B. Huttner Existence of distinct tyrosylprotein sulfotransferase genes: Molecular characterization of tyrosylprotein sulfotransferase-2 PNAS, September 15, 1998; 95(19): 11134 - 11139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Thorelli, R. J. Kaufman, and B. Dahlback The C-terminal Region of the Factor V B-domain Is Crucial for the Anticoagulant Activity of Factor V J. Biol. Chem., June 26, 1998; 273(26): 16140 - 16145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-b. Ouyang, W. S. Lane, and K. L. Moore Tyrosylprotein sulfotransferase: Purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins PNAS, March 17, 1998; 95(6): 2896 - 2901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Bundgaard, J. Vuust, and J. F. Rehfeld New Consensus Features for Tyrosine O-Sulfation Determined by Mutational Analysis J. Biol. Chem., August 29, 1997; 272(35): 21700 - 21705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Saenko and D. Scandella The Acidic Region of the Factor VIII Light Chain and the C2 Domain Together Form the High Affinity Binding Site for von Willebrand Factor J. Biol. Chem., July 18, 1997; 272(29): 18007 - 18014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Saenko, M. Shima, G. E. Gilbert, and D. Scandella Slowed Release of Thrombin-cleaved Factor VIII from von Willebrand Factor by a Monoclonal and a Human Antibody Is a Novel Mechanism for Factor VIII Inhibition J. Biol. Chem., November 1, 1996; 271(44): 27424 - 27431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Pipe and R. J. Kaufman Factor VIII C2 Domain Missense Mutations Exhibit Defective Trafficking of Biologically Functional Proteins J. Biol. Chem., October 11, 1996; 271(41): 25671 - 25676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Saenko and D. Scandella A Mechanism for Inhibition of Factor VIII Binding to Phospholipid by von Willebrand Factor J. Biol. Chem., June 9, 1995; 270(23): 13826 - 13833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. S. H. Donath, P. J. Lenting, J. A. van Mourik, and K. Mertens The Role of Cleavage of the Light Chain at Positions Arg[IMAGE] or Arg[IMAGE] in Subunit Interaction and Activation of Human Blood Coagulation Factor VIII J. Biol. Chem., February 24, 1995; 270(8): 3648 - 3655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Sarafanov, N. M. Ananyeva, M. Shima, and E. L. Saenko Cell Surface Heparan Sulfate Proteoglycans Participate in Factor VIII Catabolism Mediated by Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., April 6, 2001; 276(15): 11970 - 11979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-f. Dong, P. Ye, A. J. Schade, S. Gao, G. M. Romo, N. T. Turner, L. V. McIntire, and J. A. Lopez Tyrosine Sulfation of Glycoprotein Ibalpha . ROLE OF ELECTROSTATIC INTERACTIONS IN VON WILLEBRAND FACTOR BINDING J. Biol. Chem., May 11, 2001; 276(20): 16690 - 16694. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
