JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ala-Kokko, L.
Right arrow Articles by Prockop, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ala-Kokko, L.
Right arrow Articles by Prockop, D. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 266, Issue 22, 14175-14178, Aug, 1991

Expression of a human cartilage procollagen gene (COL2A1) in mouse 3T3 cells

L Ala-Kokko, J Hyland, C Smith, KI Kivirikko, SA Jimenez and DJ Prockop
Department of Biochemistry and Molecular Biology, Jefferson Institute of Molecular Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.

Expression in a recombinant system has been difficult to obtain for any of the major fibrillar collagens that require processing by eight or more post-translational enzymes. Here, two DNA constructs were designed so that the promoter region of the gene for the pro-alpha 1(I) chain of human type I procollagen drove expression of the human type II procollagen gene in mouse NIH 3T3 cells, a culture line that normally synthesizes type I procollagen but not any cartilage-specific protein such as type II procollagen. Both constructs were expressed as both mRNA and protein. In clones expressing the construct at high levels, the steady-state levels of mRNA and the production of type II procollagen were comparable to the mRNA levels and production of type I procollagen from the endogenous mouse genes. Comparison of clones containing the two constructs demonstrated that sequences extending 80 base pairs beyond the major polyadenylation signal of the gene are not in themselves sufficient for correct termination and 3' processing of RNA transcripts. The results strongly suggest that specific sequences present in a downstream 3.5-kilobase SphI/SphI fragment determine the termination of the transcription. Of special importance is that the system will make it possible to examine the consequences of mutations in the human type II procollagen gene on the processing of RNA transcripts and on the functional properties of the protein simply by using the genomic DNA from leukocytes or other non-cartilaginous sources.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Louneva, B. Saitta, D. J. Herrick, and S. A. Jimenez
Transcriptional Inhibition of Type I Collagen Gene Expression in Scleroderma Fibroblasts by the Antineoplastic Drug Ecteinascidin 743
J. Biol. Chem., October 10, 2003; 278(41): 40400 - 40407.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Fertala, L. Ala-Kokko, R. Wiaderkiewicz, and D. J. Prockop
Collagen II Containing a Cys Substitution for Arg-alpha 1-519. HOMOTRIMERIC MONOMERS CONTAINING THE MUTATION DO NOT ASSEMBLE INTO FIBRILS BUT ALTER THE SELF-ASSEMBLY OF THE NORMAL PROTEIN
J. Biol. Chem., March 7, 1997; 272(10): 6457 - 6464.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lamberg, T. Helaakoski, J. Myllyharju, S. Peltonen, H. Notbohm, T. Pihlajaniemi, and K. I. Kivirikko
Characterization of Human Type III Collagen Expressed in a Baculovirus System
J. Biol. Chem., May 17, 1996; 271(20): 11988 - 11995.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. P. Sokolov, L. Ala-Kokko, R. Dhulipala, M. Arita, J. S. Khillan, and D. J. Prockop
Tissue-specific Expression of the Gene for Type I Procollagen (COL1A1) in Transgenic Mice
J. Biol. Chem., April 21, 1995; 270(16): 9622 - 9629.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ghayor, J.-F. Herrouin, C. Chadjichristos, L. Ala-Kokko, M. Takigawa, J.-P. Pujol, and P. Galera
Regulation of Human COL2A1 Gene Expression in Chondrocytes. IDENTIFICATION OF C-Krox-RESPONSIVE ELEMENTS AND MODULATION BY PHENOTYPE ALTERATION
J. Biol. Chem., August 25, 2000; 275(35): 27421 - 27438.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1991 by the American Society for Biochemistry and Molecular Biology.