HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Funnell, B. E. Search for Related Content PubMed PubMed Citation Articles by Funnell, B. E. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 22, 14328-14337, Aug, 1991

The P1 plasmid partition complex at parS. The influence of Escherichia coli integration host factor and of substrate topology

BE Funnell
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

The P1 ParB protein is required for active partition and thus stable inheritance of the plasmid prophage. ParB and the Escherichia coli protein integration host factor (IHF) participate in the assembly of a partition complex at the centromere-like site parS. In this report the role of IHF in the formation of the partition complex has been explored. First, ParB protein was purified for these studies, which revealed that ParB forms a dimer in solution. Next, the IHF binding site was mapped to a 29-base pair region within parS, including the sequence TAACTGACTGTTT (which differs from the IHF consensus in two positions). IHF induced a strong bend in the DNA at its binding site. Versions of parS which have lost or damaged the IHF binding site bound ParB with greatly reduced affinity in vitro and in vivo. Measurements of binding constants showed that IHF increased ParB affinity for the wild-type parS site by about 10,000-fold. Finally, DNA supercoiling improved ParB binding in the presence of IHF but not in its absence. These observations led to the proposal that IHF and superhelicity assist ParB by promoting its precise positioning at parS, a spatial arrangement that results in a high affinity of ParB for parS.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Hoischen, M. Bussiek, J. Langowski, and S. Diekmann Escherichia coli low-copy-number plasmid R1 centromere parC forms a U-shaped complex with its binding protein ParR Nucleic Acids Res., February 2, 2008; 36(2): 607 - 615. [Abstract] [Full Text] [PDF]

				M. A. Schumacher, A. Mansoor, and B. E. Funnell Structure of a Four-way Bridged ParB-DNA Complex Provides Insight into P1 Segrosome Assembly J. Biol. Chem., April 6, 2007; 282(14): 10456 - 10464. [Abstract] [Full Text] [PDF]

				S. Ringgaard, G. Ebersbach, J. Borch, and K. Gerdes Regulatory Cross-talk in the Double par Locus of Plasmid pB171 J. Biol. Chem., February 2, 2007; 282(5): 3134 - 3145. [Abstract] [Full Text] [PDF]

				P. Kodgire, M. Dixit, and K. K. Rao ScoC and SinR Negatively Regulate epr by Corepression in Bacillus subtilis. J. Bacteriol., September 1, 2006; 188(17): 6425 - 6428. [Abstract] [Full Text] [PDF]

				A. Dabrazhynetskaya, K. Sergueev, and S. Austin Species and Incompatibility Determination within the P1par Family of Plasmid Partition Elements J. Bacteriol., September 1, 2005; 187(17): 5977 - 5983. [Abstract] [Full Text] [PDF]

				T. J. G. Fothergill, D. Barilla, and F. Hayes Protein Diversity Confers Specificity in Plasmid Segregation J. Bacteriol., April 15, 2005; 187(8): 2651 - 2661. [Abstract] [Full Text] [PDF]

				M. B. Lobocka, D. J. Rose, G. Plunkett III, M. Rusin, A. Samojedny, H. Lehnherr, M. B. Yarmolinsky, and F. R. Blattner Genome of Bacteriophage P1 J. Bacteriol., November 1, 2004; 186(21): 7032 - 7068. [Abstract] [Full Text] [PDF]

				J.-J. Hao and M. Yarmolinsky Effects of the P1 Plasmid Centromere on Expression of P1 Partition Genes J. Bacteriol., September 1, 2002; 184(17): 4857 - 4867. [Abstract] [Full Text] [PDF]

				M. Lukaszewicz, K. Kostelidou, A. A. Bartosik, G. D. Cooke, C. M. Thomas, and G. Jagura-Burdzy Functional dissection of the ParB homologue (KorB) from IncP-1 plasmid RK2 Nucleic Acids Res., February 15, 2002; 30(4): 1046 - 1055. [Abstract] [Full Text] [PDF]

				J.-Y. Bouet, J. A. Surtees, and B. E. Funnell Stoichiometry of P1 Plasmid Partition Complexes J. Biol. Chem., March 10, 2000; 275(11): 8213 - 8219. [Abstract] [Full Text] [PDF]

				N. Erdmann, T. Petroff, and B. E. Funnell Intracellular localization of P1 ParB protein depends on ParA and parS PNAS, December 21, 1999; 96(26): 14905 - 14910. [Abstract] [Full Text] [PDF]

				J. A. Surtees and B. E. Funnell P1 ParB Domain Structure Includes Two Independent Multimerization Domains J. Bacteriol., October 1, 1999; 181(19): 5898 - 5908. [Abstract] [Full Text]

				O. Rodionov, M. S. A. Lobocka,*, and M. Yarmolinsky Silencing of Genes Flanking the P1 Plasmid Centromere Science, January 22, 1999; 283(5401): 546 - 549. [Abstract] [Full Text]

				R. Magnuson and M. B. Yarmolinsky Corepression of the P1 Addiction Operon by Phd and Doc J. Bacteriol., December 1, 1998; 180(23): 6342 - 6351. [Abstract] [Full Text]

				M. J. Davey and B. E. Funnell Modulation of the P1 Plasmid Partition Protein ParA by ATP, ADP, and P1 ParB J. Biol. Chem., June 13, 1997; 272(24): 15286 - 15292. [Abstract] [Full Text] [PDF]

				J. A. Surtees and B. E. Funnell The DNA Binding Domains of P1 ParB and the Architecture of the P1 Plasmid Partition Complex J. Biol. Chem., April 6, 2001; 276(15): 12385 - 12394. [Abstract] [Full Text] [PDF]