J. Biol. Chem., Vol. 266, Issue 23, 14839-14841, 08, 1991

The interleukin-8 receptor is encoded by a neutrophil-specific cDNA clone, F3R

KM Thomas, L Taylor and J Navarro
Department of Physiology, Boston University School of Medicine, Massachusetts 02118.

Interleukin-8 (IL-8) is one of the most potent chemotactic agents for neutrophils and has been implicated as a major mediator of inflammation. The IL-8 receptor is expressed exclusively in neutrophils and belongs to the family of G-protein-coupled receptors. In a recent paper we reported the characterization of a cDNA clone, F3R, isolated from a neutrophil cDNA library and showed that it encodes a G-protein- coupled receptor which is exclusively expressed in neutrophils. We also suggested, based on expression studies in Xenopus oocytes, that the F3R protein product is an isoform of the (fMLP) receptor (Thomas, K. M., Pyun, H. Y., and Navarro, J. (1990) J. Biol. Chem. 265, 20061-20064). In this work, the F3R receptor cDNA is expressed in monkey kidney cells (COS-7) and is shown to encode the IL-8 receptor. F3R cDNA does not encode for a fMLP receptor isoform. We show conclusively that the F3R- transfected COS-7 cells express the IL-8 receptor at a density equivalent to that observed in neutrophils. The pharmacological profile of the F3R-transfected cells is the same as that of neutrophils. The apparent Kd values for binding of 125I-IL-8 to neutrophils and F3R- transfected COS-7 cell membranes were 1.2 and 1.4 nM, respectively. Antipeptide antibodies against a partial sequence of the F3R protein product specifically immunoprecipitate the IL-8 receptor from transfected cells as well as neutrophils. The molecular characterization of the IL-8 receptor should provide the basis for further studies on the identification of the binding domain of this inflammatory receptor.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. W. Hipkin, G. Deno, J. Fine, Y. Sun, B. Wilburn, X. Fan, W. Gonsiorek, and M. T. Wiekowski Cloning and Pharmacological Characterization of CXCR1 and CXCR2 from Macaca fascicularis J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 291 - 300. [Abstract] [Full Text] [PDF]

				A. L. Miller, R. M. Strieter, A. D. Gruber, S. B. Ho, and N. W. Lukacs CXCR2 Regulates Respiratory Syncytial Virus-Induced Airway Hyperreactivity and Mucus Overproduction J. Immunol., March 15, 2003; 170(6): 3348 - 3356. [Abstract] [Full Text] [PDF]

				P. L. Podolin, B. J. Bolognese, J. J. Foley, D. B. Schmidt, P. T. Buckley, K. L. Widdowson, Q. Jin, J. R. White, J. M. Lee, R. B. Goodman, et al. A Potent and Selective Nonpeptide Antagonist of CXCR2 Inhibits Acute and Chronic Models of Arthritis in the Rabbit J. Immunol., December 1, 2002; 169(11): 6435 - 6444. [Abstract] [Full Text] [PDF]

				W. C. Tsai, R. M. Strieter, B. Mehrad, M. W. Newstead, X. Zeng, and T. J. Standiford CXC Chemokine Receptor CXCR2 Is Essential for Protective Innate Host Response in Murine Pseudomonas aeruginosa Pneumonia Infect. Immun., July 1, 2000; 68(7): 4289 - 4296. [Abstract] [Full Text] [PDF]

				P. M. Murphy, M. Baggiolini, I. F. Charo, C. A. Hebert, R. Horuk, K. Matsushima, L. H. Miller, J. J. Oppenheim, and C. A. Power International Union of Pharmacology. XXII. Nomenclature for Chemokine Receptors Pharmacol. Rev., March 1, 2000; 52(1): 145 - 176. [Abstract] [Full Text] [PDF]

				B. Mehrad, R. M. Strieter, T. A. Moore, W. C. Tsai, S. A. Lira, and T. J. Standiford CXC Chemokine Receptor-2 Ligands Are Necessary Components of Neutrophil-Mediated Host Defense in Invasive Pulmonary Aspergillosis J. Immunol., December 1, 1999; 163(11): 6086 - 6094. [Abstract] [Full Text] [PDF]

				D. C. Maestes, R. M. Potter, and E. R. Prossnitz Differential Phosphorylation Paradigms Dictate Desensitization and Internalization of the N-Formyl Peptide Receptor J. Biol. Chem., October 15, 1999; 274(42): 29791 - 29795. [Abstract] [Full Text] [PDF]

				K. Suetomi, Z. Lu, T. Heck, T. G. Wood, D. J. Prusak, K. J. Dunn, and J. Navarro Differential Mechanisms of Recognition and Activation of Interleukin-8 Receptor Subtypes J. Biol. Chem., April 23, 1999; 274(17): 11768 - 11772. [Abstract] [Full Text] [PDF]

				N. C. Wilkinson and J. Navarro PU.1 Regulates the CXCR1 Promoter J. Biol. Chem., January 1, 1999; 274(1): 438 - 443. [Abstract] [Full Text] [PDF]

				J. S. Mills, H. M. Miettinen, D. Barnidge, M. J. Vlases, S. Wimer-Mackin, E. A. Dratz, J. Sunner, and A. J. Jesaitis Identification of a Ligand Binding Site in the Human Neutrophil Formyl Peptide Receptor Using a Site-specific Fluorescent Photoaffinity Label and Mass Spectrometry J. Biol. Chem., April 24, 1998; 273(17): 10428 - 10435. [Abstract] [Full Text] [PDF]

				X. Sanchez, K. Suetomi, B. Cousins-Hodges, J. K. Horton, and J. Navarro CXC Chemokines Suppress Proliferation of Myeloid Progenitor Cells by Activation of the CXC Chemokine Receptor 2 J. Immunol., January 15, 1998; 160(2): 906 - 910. [Abstract] [Full Text] [PDF]

				G. N. Prado, H. Suzuki, N. Wilkinson, B. Cousins, and J. Navarro Role of the C Terminus of the Interleukin 8Receptor in Signal Transduction and Internalization J. Biol. Chem., August 9, 1996; 271(32): 19186 - 19190. [Abstract] [Full Text] [PDF]

				T. J. Daly, G. J. LaRosa, S. Dolich, T. E. Maione, S. Cooper, and H. E. Broxmeyer High Activity Suppression of Myeloid Progenitor Proliferation by Chimeric Mutants of Interleukin 8 and Platelet Factor 4 J. Biol. Chem., October 6, 1995; 270(40): 23282 - 23292. [Abstract] [Full Text] [PDF]

				K Rajarathnam, B. Sykes, C. Kay, B Dewald, T Geiser, M Baggiolini, and I Clark-Lewis Neutrophil activation by monomeric interleukin-8 Science, April 1, 1994; 264(5155): 90 - 92. [Abstract] [PDF]

				D Wu, G. LaRosa, and M. Simon G protein-coupled signal transduction pathways for interleukin-8 Science, July 2, 1993; 261(5117): 101 - 103. [Abstract] [PDF]

				P. H. Naccache, S. Levasseur, G. Lachance, S. Chakravarti, S. G. Bourgoin, and S. R. McColl Stimulation of Human Neutrophils by Chemotactic Factors Is Associated with the Activation of Phosphatidylinositol 3-Kinase gamma J. Biol. Chem., July 28, 2000; 275(31): 23636 - 23641. [Abstract] [Full Text] [PDF]