| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 23, 15318-15324, 08, 1991
S Gunwar, F Ballester, R Kalluri, J Timoneda, AM Chonko, SJ Edwards, ME Noelken and BG Hudson
The noncollagenous (NC1) domain hexamer of glomerular basement membrane
(GBM) collagen is composed of a multiplicity of monomeric and dimeric
subunits, and specific subunits are the targets for anti-GBM autoantibodies
of patients with Goodpasture (GP) syndrome. The identity of GBM monomers
has been established and the alpha 3(IV)NC1 monomer identified as the one
that binds GP antibodies (Gunwar, S., Saus, J., Noelken, M. E., and Hudson,
B. G. (1990) J. Biol. Chem. 265, 5466- 5469). In the present study, the
chain origin of 25 dimeric components and the identity of those that bound
the anti-GBM antibodies from two GP patients were determined. This was
accomplished by NH2-terminal sequence analysis and immunoblotting analysis
of dimeric components that were resolved by two-dimensional electrophoresis
in combination with high pressure liquid chromatography. The results
revealed that (a) the components are mainly homodimers of the NC1 domains
of alpha 1, alpha 2, alpha 3, alpha 4, and probably alpha 5 chains of
collagen IV, reflecting a specificity of promoter-promoter association and
(b) each homodimer had several size and charge isoforms. The GP antibodies
bound exclusively to both alpha 3(IV)NC1 monomers and dimers and not to
other basement membrane constituents. These findings provided new insights
about the structure of GBM collagen and together with our previous findings
firmly established the alpha 3(IV) chain as the target for the anti-GBM
antibodies that mediate glomerulonephritis and pulmonary hemorrhage in
patients with Goodpasture syndrome.
Glomerular basement membrane. Identification of dimeric subunits of the noncollagenous domain (hexamer) of collagen IV and the Goodpasture antigen
Department of Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Sugimoto, T. M. Mundel, M. Sund, L. Xie, D. Cosgrove, and R. Kalluri Bone-marrow-derived stem cells repair basement membrane collagen defects and reverse genetic kidney disease PNAS, May 9, 2006; 103(19): 7321 - 7326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Khoshnoodi, K. Sigmundsson, J.-P. Cartailler, O. Bondar, M. Sundaramoorthy, and B. G. Hudson Mechanism of Chain Selection in the Assembly of Collagen IV: A PROMINENT ROLE FOR THE {alpha}2 CHAIN J. Biol. Chem., March 3, 2006; 281(9): 6058 - 6069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Vanacore, S. Shanmugasundararaj, D. B. Friedman, O. Bondar, B. G. Hudson, and M. Sundaramoorthy The {alpha}1.{alpha}2 Network of Collagen IV: REINFORCED STABILIZATION OF THE NONCOLLAGENOUS DOMAIN-1 BY NONCOVALENT FORCES AND THE ABSENCE OF MET-LYS CROSS-LINKS J. Biol. Chem., October 22, 2004; 279(43): 44723 - 44730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Hudson The Molecular Basis of Goodpasture and Alport Syndromes: Beacons for the Discovery of the Collagen IV Family J. Am. Soc. Nephrol., October 1, 2004; 15(10): 2514 - 2527. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bhattacharya, R. Kalluri, D. J. Orten, W. J. Kimberling, and D. Cosgrove A domain-specific usherin/collagen IV interaction may be required for stable integration into the basement membrane superstructure J. Cell Sci., January 15, 2004; 117(2): 233 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zeisberg, Y. Maeshima, B. Mosterman, and R. Kalluri Renal Fibrosis : Extracellular Matrix Microenvironment Regulates Migratory Behavior of Activated Tubular Epithelial Cells Am. J. Pathol., June 1, 2002; 160(6): 2001 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zeisberg, G. Bonner, Y. Maeshima, P. Colorado, G. A. Muller, F. Strutz, and R. Kalluri Renal Fibrosis : Collagen Composition and Assembly Regulates Epithelial-Mesenchymal Transdifferentiation Am. J. Pathol., October 1, 2001; 159(4): 1313 - 1321. [Abstract] [Full Text]
|
|
|
|
|
|
R. Kalluri and D. Cosgrove Assembly of Type IV Collagen. INSIGHTS FROM alpha 3(IV) COLLAGEN-DEFICIENT MICE J. Biol. Chem., April 21, 2000; 275(17): 12719 - 12724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Kamphaus, P. C. Colorado, D. J. Panka, H. Hopfer, R. Ramchandran, A. Torre, Y. Maeshima, J. W. Mier, V. P. Sukhatme, and R. Kalluri Canstatin, a Novel Matrix-derived Inhibitor of Angiogenesis and Tumor Growth J. Biol. Chem., January 14, 2000; 275(2): 1209 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-O. Netzer, A. Leinonen, A. Boutaud, D.-B. Borza, P. Todd, S. Gunwar, J. P. M. Langeveld, and B. G. Hudson The Goodpasture Autoantigen. MAPPING THE MAJOR CONFORMATIONAL EPITOPE(S) OF alpha 3(IV) COLLAGEN TO RESIDUES 17-31 AND 127-141 OF THE NC1 DOMAIN J. Biol. Chem., April 16, 1999; 274(16): 11267 - 11274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gunwar, F. Ballester, M. E. Noelken, Y. Sado, Y. Ninomiya, and B. G. Hudson Glomerular Basement Membrane. IDENTIFICATION OF A NOVEL DISULFIDE-CROSS-LINKED NETWORK OF alpha 3, alpha 4, AND alpha 5 CHAINS OF TYPE IV COLLAGEN AND ITS IMPLICATIONS FOR THE PATHOGENESIS OF ALPORT SYNDROME J. Biol. Chem., April 10, 1998; 273(15): 8767 - 8775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Z. Kahsai, G. C. Enders, S. Gunwar, C. Brunmark, J. Wieslander, R. Kalluri, J. Zhou, M. E. Noelken, and B. G. Hudson Seminiferous Tubule Basement Membrane. COMPOSITION AND ORGANIZATION OF TYPE IV COLLAGEN CHAINS, AND THE LINKAGE OF alpha 3(IV) AND alpha 5(IV) CHAINS J. Biol. Chem., July 4, 1997; 272(27): 17023 - 17032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Cosgrove, D T Meehan, J A Grunkemeyer, J M Kornak, R Sayers, W J Hunter, and G C Samuelson Collagen COL4A3 knockout: a mouse model for autosomal Alport syndrome. Genes & Dev., December 1, 1996; 10(23): 2981 - 2992. [Abstract] [PDF]
|
|
|
|
|
|
P. S. Thorner, K. Zheng, R. Kalluri, R. Jacobs, and B. G. Hudson Coordinate Gene Expression of the alpha 3, alpha 4, and alpha 5 Chains of Collagen Type IV. EVIDENCE FROM A CANINE MODEL OF X-LINKED NEPHRITIS WITH A COL4A5 GENE MUTATION J. Biol. Chem., June 7, 1996; 271(23): 13821 - 13828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kalluri, M. J. Sun, B. G. Hudson, and E. G. Neilson The Goodpasture Autoantigen J. Biol. Chem., April 12, 1996; 271(15): 9062 - 9068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Zhou, T Mochizuki, H Smeets, C Antignac, P Laurila, A de Paepe, K Tryggvason, and S. Reeders Deletion of the paired alpha 5(IV) and alpha 6(IV) collagen genes in inherited smooth muscle tumors Science, August 27, 1993; 261(5125): 1167 - 1169. [Abstract] [PDF]
|
|
|
|
|
|
D.-B. Borza, O. Bondar, Y. Ninomiya, Y. Sado, I. Naito, P. Todd, and B. G. Hudson The NC1 Domain of Collagen IV Encodes a Novel Network Composed of the alpha 1, alpha 2, alpha 5, and alpha 6 Chains in Smooth Muscle Basement Membranes J. Biol. Chem., July 20, 2001; 276(30): 28532 - 28540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kalluri, L. G. Cantley, D. Kerjaschki, and E. G. Neilson Reactive Oxygen Species Expose Cryptic Epitopes Associated with Autoimmune Goodpasture Syndrome J. Biol. Chem., June 23, 2000; 275(26): 20027 - 20032. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
