| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 23, 15493-15497, 08, 1991
Y Singh, KR Klimpel, CP Quinn, VK Chaudhary and SH Leppla
Anthrax toxin consists of three separate proteins produced by Bacillus
anthracis: protective antigen (PA), lethal factor (LF), and edema factor
(EF). Previous work showed that the process by which these proteins damage
eukaryotic cells begins with binding of PA (83 kDa) to cell surface
receptors. PA is then cleaved by a cell surface protease so as to expose a
high-affinity binding site for LF or EF on the COOH- terminal,
receptor-bound, 63-kilodalton fragment. In this report we more closely
define a region of PA involved in receptor binding. The gene encoding PA
was mutagenized so as to delete 3, 5, 7, 12, or 14 amino acids from the
carboxyl terminus of the protein, and the truncated PA variants were
purified from Bacillus subtilis or Escherichia coli. Deletion of 3, 5, or 7
amino acids reduced the binding of PA to cells and the subsequent toxicity
of the PA.LF complex to J774A.1 cells and also the ability to cause EF
binding to cells. Deletion of 12 or 14 amino acids completely eliminated
all these activities. These results show that the carboxy terminus
comprises or is part of the receptor-binding domain of PA.
The carboxyl-terminal end of protective antigen is required for receptor binding and anthrax toxin activity
Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. T. Albrecht, H. Li, E. D. Williamson, C. S. LeButt, H. C. Flick-Smith, C. P. Quinn, H. Westra, D. Galloway, A. Mateczun, S. Goldman, et al. Human Monoclonal Antibodies against Anthrax Lethal Factor and Protective Antigen Act Independently To Protect against Bacillus anthracis Infection and Enhance Endogenous Immunity to Anthrax Infect. Immun., November 1, 2007; 75(11): 5425 - 5433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. A. Rainey, D. J. Wigelsworth, P. L. Ryan, H. M. Scobie, R. J. Collier, and J. A. T. Young Receptor-specific requirements for anthrax toxin delivery into cells PNAS, September 13, 2005; 102(37): 13278 - 13283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brossier, M. Levy, A. Landier, P. Lafaye, and M. Mock Functional Analysis of Bacillus anthracis Protective Antigen by Using Neutralizing Monoclonal Antibodies Infect. Immun., November 1, 2004; 72(11): 6313 - 6317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Barth, K. Aktories, M. R. Popoff, and B. G. Stiles Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins Microbiol. Mol. Biol. Rev., September 1, 2004; 68(3): 373 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Marvaud, T. Smith, M. L. Hale, M. R. Popoff, L. A. Smith, and B. G. Stiles Clostridium perfringens Iota-Toxin: Mapping of Receptor Binding and Ia Docking Domains on Ib Infect. Immun., April 1, 2001; 69(4): 2435 - 2441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mogridge, M. Mourez, and R. J. Collier Involvement of Domain 3 in Oligomerization by the Protective Antigen Moiety of Anthrax Toxin J. Bacteriol., March 15, 2001; 183(6): 2111 - 2116. [Abstract] [Full Text]
|
|
|
|
|
|
S. Cohen, I. Mendelson, Z. Altboum, D. Kobiler, E. Elhanany, T. Bino, M. Leitner, I. Inbar, H. Rosenberg, Y. Gozes, et al. Attenuated Nontoxinogenic and Nonencapsulated Recombinant Bacillus anthracis Spore Vaccines Protect against Anthrax Infect. Immun., August 1, 2000; 68(8): 4549 - 4558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Blocker, H. Barth, E. Maier, R. Benz, J. T. Barbieri, and K. Aktories The C Terminus of Component C2II of Clostridium botulinum C2 Toxin Is Essential for Receptor Binding Infect. Immun., August 1, 2000; 68(8): 4566 - 4573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brossier, M. Weber-Levy, M. Mock, and J.-C. Sirard Role of Toxin Functional Domains in Anthrax Pathogenesis Infect. Immun., April 1, 2000; 68(4): 1781 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Eckhardt, H. Barth, D. Blocker, and K. Aktories Binding of Clostridium botulinum C2 Toxin to Asparagine-linked Complex and Hybrid Carbohydrates J. Biol. Chem., January 28, 2000; 275(4): 2328 - 2334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Cirino, D. Sblattero, D. Allen, S. R. Peterson, J. D. Marks, P. J. Jackson, A. Bradbury, and B. E. Lehnert Disruption of Anthrax Toxin Binding with the Use of Human Antibodies and Competitive Inhibitors Infect. Immun., June 1, 1999; 67(6): 2957 - 2963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Singh, K. R. Klimpel, S. Goel, P. K. Swain, and S. H. Leppla Oligomerization of Anthrax Toxin Protective Antigen and Binding of Lethal Factor during Endocytic Uptake into Mammalian Cells Infect. Immun., April 1, 1999; 67(4): 1853 - 1859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Varughese, A. V. Teixeira, S. Liu, and S. H. Leppla Identification of a Receptor-Binding Region within Domain 4 of the Protective Antigen Component of Anthrax Toxin Infect. Immun., April 1, 1999; 67(4): 1860 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Barnard and A. M. Friedlander Vaccination against Anthrax with Attenuated Recombinant Strains of Bacillus anthracis That Produce Protective Antigen Infect. Immun., February 1, 1999; 67(2): 562 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brossier, J.-C. Sirard, C. Guidi-Rontani, E. Duflot, and M. Mock Functional Analysis of the Carboxy-Terminal Domain of Bacillus anthracis Protective Antigen Infect. Immun., February 1, 1999; 67(2): 964 - 967. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
