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J. Biol. Chem., Vol. 266, Issue 24, 15559-15562, 08, 1991
KG Langford, SY Shai, TE Howard, MJ Kovac, PA Overbeek and KE Bernstein
There are two isozymes of angiotensin-converting enzyme (ACE), one produced
by somatic tissues and a smaller protein synthesized by developing
spermatozoa (testis ACE). To investigate the molecular control of testis
ACE, we generated mice transgenic for a construct containing a putative
testis-specific ACE promoter linked to the Escherichia coli reporter gene
encoding beta-galactosidase. The transgenic mice express beta-galactosidase
protein and RNA only within the testis. Histochemical analysis of the
transgenic mice shows co- localization of beta-galactosidase protein and
endogenous ACE within elongating spermatozoa. These studies demonstrate
that transcription of testis ACE is controlled by a strong intragenic
testis-specific promoter that is contained within a 698-base pair fragment
immediately upstream from the transcription start site of testis ACE.
Characterization of the testis ACE promoter may provide insights into the
molecular mechanisms controlling cell stage-specific gene expression in the
male germ line.
Transgenic mice demonstrate a testis-specific promoter for angiotensin- converting enzyme
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30322.
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