| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 26, 17004-17010, 09, 1991
EJ Husten and BA Eipper
The biosynthesis of alpha-amidated peptides from their glycine-extended
precursors is catalyzed by the sequential action of peptidylglycine
alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha- hydroxyglycine
alpha-amidating lyase (PAL). The two enzymes are part of a bifunctional,
integral membrane protein precursor, peptidylglycine alpha-amidating
monooxygenase (PAM). The major forms of PAM mRNA in the adult rat atrium
differ by the presence or absence of optional exon A, a 315-nucleotide
segment separating the PHM and PAL domains. Using antipeptide antibodies
specific to the PHM, exon A, PAL, and cytoplasmic domains of rat PAM,
carbonate-washed atrial membranes were found to contain proteins
corresponding to rPAM-1 and rPAM-2. Digestion of atrial membranes with a
variety of endoproteinases released PHM and PAL catalytic activities.
Dose-response curves indicated that both catalytic activities were
extremely resistant to inactivation by trypsin. Endoproteolytic digestion
of atrial membranes with trypsin, chymotrypsin, elastase, thermolysin, or
endoproteinase Lys-C generated a 35-kDa PHM fragment. Digestion with
trypsin, elastase, thermolysin, or endoproteinase Lys-C generated a 42-kDa
PAL fragment. In contrast to the stability exhibited by the PHM and PAL
domains, the cytoplasmic domain of PAM was destroyed by most of the
enzymes; only digestion with endoproteinase Lys-C generated a stable
fragment. Digestion with endoproteinase Arg-C removed the carboxyl-terminal
tail from PAM but failed to release the PHM or PAL domains from the
membranes. The PHM fragments generated by some of the endoproteinases
showed a tendency to adhere to the membranes. Thus the bifunctional PAM
protein consists of independent catalytic domains separated from each other
and from the putative transmembrane domain by flexible regions accessible
to attack by a wide variety of endoproteinases.
The membrane-bound bifunctional peptidylglycine alpha-amidating monooxygenase protein. Exploration of its domain structure through limited proteolysis
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Soule, V. Stout, W. D. Swingley, J. C. Meeks, and F. Garcia-Pichel Molecular Genetics and Genomic Analysis of Scytonemin Biosynthesis in Nostoc punctiforme ATCC 29133 J. Bacteriol., June 15, 2007; 189(12): 4465 - 4472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Steveson, G. D. Ciccotosto, X.-M. Ma, G. P. Mueller, R. E. Mains, and B. A. Eipper Menkes Protein Contributes to the Function of Peptidylglycine {alpha}-Amidating Monooxygenase Endocrinology, January 1, 2003; 144(1): 188 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Steveson, G. C. Zhao, H. T. Keutmann, R. E. Mains, and B. A. Eipper Access of a Membrane Protein to Secretory Granules Is Facilitated by Phosphorylation J. Biol. Chem., October 19, 2001; 276(43): 40326 - 40337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Alam, T. C. Steveson, R. C. Johnson, N. Bäck, B. Abraham, R. E. Mains, and B. A. Eipper Signaling Mediated by the Cytosolic Domain of Peptidylglycine {alpha}-Amidating Monooxygenase Mol. Biol. Cell, March 1, 2001; 12(3): 629 - 644. [Abstract] [Full Text]
|
|
|
|
|
|
R. El Meskini, R. E. Mains, and B. A. Eipper Cell Type-Specific Metabolism of Peptidylglycine {alpha}-Amidating Monooxygenase in Anterior Pituitary Endocrinology, August 1, 2000; 141(8): 3020 - 3034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Ciccotosto, T. A. Hand, R. E. Mains, and B. A. Eipper Breeding Stock-Specific Variation in Peptidylglycine {alpha}-Amidating Monooxygenase Messenger Ribonucleic Acid Splicing in Rat Pituitary Endocrinology, February 1, 2000; 141(2): 476 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bruzzaniti, R. Marx, and R. E. Mains Activation and Routing of Membrane-tethered Prohormone Convertases 1 and 2 J. Biol. Chem., August 27, 1999; 274(35): 24703 - 24713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Steveson, H. T. Keutmann, R. E. Mains, and B. A. Eipper Phosphorylation of Cytosolic Domain Ser937 Affects Both Biosynthetic and Endocytic Trafficking of Peptidylglycine alpha -Amidating Monooxygenase J. Biol. Chem., July 23, 1999; 274(30): 21128 - 21138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. SPIJKER, A. B. SMIT, B. A. EIPPER, A. MALIK, R. E. MAINS, and W. P. M. GERAERTS A molluscan peptide {alpha}-amidating enzyme precursor that generates five distinct enzymes FASEB J, April 1, 1999; 13(6): 735 - 748. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. D. Ciccotosto, M. R. Schiller, B. A. Eipper, and R. E. Mains Induction of Integral Membrane PAM Expression in AtT-20 Cells Alters the Storage and Trafficking of POMC and PC1 J. Cell Biol., February 8, 1999; 144(3): 459 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Marx and R. E. Mains Adenovirally Encoded Prohormone Convertase-1 Functions in Atrial Myocyte Large Dense Core Vesicles Endocrinology, December 1, 1997; 138(12): 5108 - 5118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. El Meskini, F. Boudouresque, and L'H. Ouafik Estrogen Regulation of Peptidylglycine {alpha}-Amidating Monooxygenase Messenger Ribonucleic Acid Levels by a Nuclear Posttranscriptional Event Endocrinology, December 1, 1997; 138(12): 5256 - 5265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Oldham, C. Li, J. Feng, R. O. Scott, W. Z. Wang, A. B. Moore, P. R. Girard, J. Huang, R. B. Caldwell, R. W. Caldwell, et al. Amidative peptide processing and vascular function Am J Physiol Cell Physiol, December 1, 1997; 273(6): C1908 - C1914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Kolhekar, M. S. Roberts, N. Jiang, R. C. Johnson, R. E. Mains, B. A. Eipper, and P. H. Taghert Neuropeptide Amidation in Drosophila: Separate Genes Encode the Two Enzymes Catalyzing Amidation J. Neurosci., February 15, 1997; 17(4): 1363 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. El Meskini, C. Delfino, F. Boudouresque, M. Hery, C. Oliver, and L'H. Ouafik Estrogen Regulation of Peptidylglycine {alpha}-Amidating Monooxygenase Expression in Anterior Pituitary Gland Endocrinology, January 1, 1997; 138(1): 379 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ping, C. E. Mounier, and S. W. May Reaction Versus Subsite Stereospecificity of Peptidylglycine alpha-Monooxygenase and Peptidylamidoglycolate Lyase, the Two Enzymes Involved in Peptide Amidation J. Biol. Chem., December 8, 1995; 270(49): 29250 - 29255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Yun and B. A. Eipper Addition of an Endoplasmic Reticulum Retention/Retrieval Signal Does Not Block Maturation of Enzymatically Active Peptidylglycine [IMAGE]-Amidating Monooxygenase J. Biol. Chem., June 23, 1995; 270(25): 15412 - 15416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Oyarce and B. Eipper Identification of subcellular compartments containing peptidylglycine alpha-amidating monooxygenase in rat anterior pituitary J. Cell Sci., January 1, 1995; 108(1): 287 - 297. [Abstract] [PDF]
|
|
|
|
|
|
R. El Meskini, G. J. Galano, R. Marx, R. E. Mains, and B. A. Eipper Targeting of Membrane Proteins to the Regulated Secretory Pathway in Anterior Pituitary Endocrine Cells J. Biol. Chem., January 26, 2001; 276(5): 3384 - 3393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Oyarce, T. C. Steveson, L. Jin, and B. A. Eipper Dopamine beta -Monooxygenase Signal/Anchor Sequence Alters Trafficking of Peptidylglycine alpha -Hydroxylating Monooxygenase J. Biol. Chem., August 24, 2001; 276(35): 33265 - 33272. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
