HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beyer, P. Articles by Nievelstein, V. Search for Related Content PubMed PubMed Citation Articles by Beyer, P. Articles by Nievelstein, V. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 26, 17072-17078, 09, 1991

On the mechanism of the lycopene isomerase/cyclase reaction in Narcissus pseudonarcissus L. chromoplasts

P Beyer, U Kroncke and V Nievelstein
Institut fur Biologie II, Universitat Freiburg, Federal Republic of Germany.

The lycopene cyclase reaction was investigated in Narcissus pseudonarcissus L. membranes using the terminal desaturation product of the system, 7,9,9',7'-tetra-cis-lycopene (prolycopene) as substrate. The enzymatically formed bicyclic carotenes were trans configured, so the overall reaction consists of two individual processes, isomerization and cyclization. The two reactions were experimentally distinguished by use of the inhibitor [2-(p- chlorophenylthio)triethylammonium chloride. NADPH, the essential cofactor for the overall reaction, originates from the plastid glycolytic pathway. It is shown that NADPH exhibits its function at the stage of isomerization and is also able to promote reduction of the prolycopene substrate in vitro.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Isaacson, I. Ohad, P. Beyer, and J. Hirschberg Analysis in Vitro of the Enzyme CRTISO Establishes a Poly-cis-Carotenoid Biosynthesis Pathway in Plants Plant Physiology, December 1, 2004; 136(4): 4246 - 4255. [Abstract] [Full Text] [PDF]

				P. Beyer, S. Al-Babili, X. Ye, P. Lucca, P. Schaub, R. Welsch, and I. Potrykus Golden Rice: Introducing the {beta}-Carotene Biosynthesis Pathway into Rice Endosperm by Genetic Engineering to Defeat Vitamin A Deficiency J. Nutr., March 1, 2002; 132(3): 506S - 510. [Abstract] [Full Text] [PDF]

				T. Isaacson, G. Ronen, D. Zamir, and J. Hirschberg Cloning of tangerine from Tomato Reveals a Carotenoid Isomerase Essential for the Production of {beta}-Carotene and Xanthophylls in Plants PLANT CELL, February 1, 2002; 14(2): 333 - 342. [Abstract] [Full Text] [PDF]