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J. Biol. Chem., Vol. 266, Issue 26, 17212-17217, 09, 1991
JM Baltz, JD Biggers and C Lechene
Mouse embryos at the two-cell stage are able to recover from an alkaline
load. We found that this recovery is mediated by sodium- independent
bicarbonate/chloride exchange: intracellular pH (pHi) recovery from
alkaline load is inhibited by the anion exchange inhibitor
4,4'-diisothiocyanostilbene disulfonic acid, lack of bicarbonate, or lack
of chloride. The dependence of the pHi recovery on extracellular chloride
concentration exhibits Michaelis-Menten kinetics. Furthermore, uptake of
chloride is inhibited in a dose- dependent manner by extracellular
bicarbonate. The Km for external chloride was found to be about 3 mM, with
a Ki for external bicarbonate of about 2 mM. The exchanger is active above
approximately pH 7.15. These results demonstrate that mouse embryos at the
two-cell stage possess a sodium-independent bicarbonate/chloride exchange
mechanism that is similar to that found in other mammalian cells. This
bicarbonate/chloride exchanger appears to be the sole pHi-regulatory
mechanism in the two-cell stage mouse embryo, since our previous results
have shown that there are apparently no specific mechanisms active in these
cells for relieving acid loads.
Relief from alkaline load in two-cell stage mouse embryos by bicarbonate/chloride exchange
Laboratory of Human Reproduction and Reproductive Biology, Harvard Medical School, Boston, Massachusetts 02115.
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