| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 27, 17826-17831, Sep, 1991
D Wang, WC Mentzer, T Cameron and RM Johnson
A 31-kDa human erythrocyte integral protein, band 7.2b, has been purified
to better than 95% homogeneity. The polypeptide was found to be insoluble
in most detergents and was isolated in denatured form by gel filtration in
the presence of sodium dodecyl sulfate and preparative electrophoresis. In
intact erythrocytes that were equilibrated with 32Pi, band 7.2b was
phosphorylated in response to exogenous dibutyryl cAMP. The peptide is also
palmitylated, as shown by its incorporation of radioactivity when intact
erythrocytes were incubated with [9,10-3H]palmitic acid. Antisera to band
7.2b were raised in rabbits, and these antibodies cross-react with 31-kDa
polypeptides in human liver and kidney. Immunoblots of red cells from a
number of other species were negative, with the exception of a cross-
reacting 23-kDa polypeptide in rat erythrocyte membranes. Band 7.2b was
absent in erythrocyte membranes from an individual with overhydrated
hereditary stomatocytes.
Purification of band 7.2b, a 31-kDa integral phosphoprotein absent in hereditary stomatocytosis
Department of Biochemistry, Wayne Street University, Detroit, Michigan 48201.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Umlauf, M. Mairhofer, and R. Prohaska Characterization of the Stomatin Domain Involved in Homo-oligomerization and Lipid Raft Association J. Biol. Chem., August 18, 2006; 281(33): 23349 - 23356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Price, R. J. Thompson, J. O. Eshcol, J. A. Wemmie, and C. J. Benson Stomatin Modulates Gating of Acid-sensing Ion Channels J. Biol. Chem., December 17, 2004; 279(51): 53886 - 53891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kumar, Y.-P. Xiao, P. J. Laipis, B. S. Fletcher, and S. C. Frost Glucose deprivation enhances targeting of GLUT1 to lipid rafts in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, April 1, 2004; 286(4): E568 - E576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mairhofer, M. Steiner, W. Mosgoeller, R. Prohaska, and U. Salzer Stomatin is a major lipid-raft component of platelet alpha granules Blood, July 18, 2002; 100(3): 897 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Salzer, P. Hinterdorfer, U. Hunger, C. Borken, and R. Prohaska Ca++-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin Blood, April 1, 2002; 99(7): 2569 - 2577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Z. Zhang, W. Abbud, R. Prohaska, and F. Ismail-Beigi Overexpression of stomatin depresses GLUT-1 glucose transporter activity Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1277 - C1283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Salzer and R. Prohaska Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts Blood, February 15, 2001; 97(4): 1141 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, C. Paszty, T. Turetsky, S. Tsai, F. A. Kuypers, G. Lee, P. Cooper, P. G. Gallagher, M. E. Stevens, E. Rubin, et al. Stomatocytosis Is Absent in "Stomatin"-Deficient Murine Red Blood Cells Blood, April 1, 1999; 93(7): 2404 - 2410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Snyers, E. Umlauf, and R. Prohaska Oligomeric Nature of the Integral Membrane Protein Stomatin J. Biol. Chem., July 3, 1998; 273(27): 17221 - 17226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Gallagher and B. G. Forget Structure, Organization, and Expression of the Human Band 7.2b Gene, a Candidate Gene for Hereditary Hydrocytosis J. Biol. Chem., November 3, 1995; 270(44): 26358 - 26363. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
