HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rayson, B. M. Search for Related Content PubMed PubMed Citation Articles by Rayson, B. M. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 32, 21335-21338, Nov, 1991

[Ca2+]i regulates transcription rate of the Na+/K(+)-ATPase alpha 1 subunit

BM Rayson
Department of Physiology, Cornell University Medical College, New York, New York 10021.

The Na+/K(+)-ATPase enzyme is a pivotal regulator of intracellular electrolyte levels in animal cells. Changes in the rate of its synthesis have been demonstrated to mediate a number of physiological responses. The cellular mechanisms implicated in such regulatory responses though remain poorly defined. Specifically, no intracellular mediators have previously been established. We now describe for the first time, one putative mediator: [Ca2+]i. Elevations in [Ca2+]i stimulate levels of mRNA alpha 1 and mRNA beta 1, sequences which encode both the catalytic subunit of the enzyme, the alpha subunit, and the glycosylated subunit, the beta subunit. These results suggest that elevations in [Ca2+]i modulate the synthetic rate of the active enzyme, and consequently changes in [Ca2+]i may mediate the regulation of its synthetic rate in a range of physiological settings. The mRNA alpha 1 response to the elevation of [Ca2+]i appears, at least partly, to reflect an increase in transcription rate. However, no such increase in transcription rate of the beta 1 subunit gene was detectable. Thus the mRNA beta 1 response, under conditions of elevated [Ca2+]i, may be explained by a decrease in specific mRNA degradation rate.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. T. Murphy, W. A. Macdonald, M. J. McKenna, and T. Clausen Ionic mechanisms of excitation-induced regulation of Na+-K+-ATPase mRNA expression in isolated rat EDL muscle Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1397 - R1406. [Abstract] [Full Text] [PDF]

				K. T. Murphy, A. C. Petersen, C. Goodman, X. Gong, J. A. Leppik, A. P. Garnham, D. Cameron-Smith, R. J. Snow, and M. J. McKenna Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R414 - R424. [Abstract] [Full Text] [PDF]

				N. Nordsborg, M. Thomassen, C. Lundby, H. Pilegaard, and J. Bangsbo Contraction-induced increases in Na+-K+-ATPase mRNA levels in human skeletal muscle are not amplified by activation of additional muscle mass Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R84 - R91. [Abstract] [Full Text] [PDF]

				K. T. Murphy, R. J. Snow, A. C. Petersen, R. M. Murphy, J. Mollica, J. S. Lee, A. P. Garnham, R. J. Aughey, J. A. Leppik, I. Medved, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle J. Physiol., April 15, 2004; 556(2): 507 - 519. [Abstract] [Full Text] [PDF]

				N. Sevieux, J. Alam, and E. Songu-Mize Effect of cyclic stretch on {alpha}-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1555 - C1560. [Abstract] [Full Text] [PDF]

				X. Liu, L. J. Hymel, and E. Songu-Mize Role of Na+ and Ca2+ in stretch-induced Na+-K+-ATPase alpha -subunit regulation in aortic smooth muscle cells Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H83 - H89. [Abstract] [Full Text] [PDF]

				E. Songu-Mize, X. Liu, J. E. Stones, and L. J. Hymel Regulation of Na+,K+-ATPase {alpha}-Subunit Expression by Mechanical Strain in Aortic Smooth Muscle Cells Hypertension, March 1, 1996; 27(3): 827 - 832. [Abstract] [Full Text]

				Y. Zhuang, C. Wendt, and G. Gick Regulation of Na,K-ATPase beta 1 Subunit Gene Transcription by Low External Potassium in Cardiac Myocytes. ROLE OF Sp1 AND Sp3 J. Biol. Chem., July 28, 2000; 275(31): 24173 - 24184. [Abstract] [Full Text] [PDF]