| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 33, 22075-22078, 11, 1991
PK Chakraborti, MJ Garabedian, KR Yamamoto and SS Simons Jr
Almost all modifications of the steroid binding domain of glucocorticoid
receptors are known to cause a reduction or loss of steroid binding
activity. Nonetheless, we now report that mutations of cysteine 656 of the
rat receptor, which was previously suspected to be a crucial amino acid for
the binding process, have produced "super" receptors. These receptors
displayed an increased affinity for glucocorticoid steroids and a decreased
relative affinity for cross- reacting steroids such as progesterone and
aldosterone. The increased in vitro affinity of the super receptors was
maintained in a whole cell bioassay. These results indicate that additional
modifications of the glucocorticoid receptor, and probably the other
steroid receptors, may further increase the binding affinity and/or
specificity.
Creation of "super" glucocorticoid receptors by point mutations in the steroid binding domain
Steroid Hormones Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. v. d. Brandt, F. Luhder, K. G. McPherson, K. L. de Graaf, D. Tischner, S. Wiehr, T. Herrmann, R. Weissert, R. Gold, and H. M. Reichardt Enhanced Glucocorticoid Receptor Signaling in T Cells Impacts Thymocyte Apoptosis and Adaptive Immune Responses Am. J. Pathol., March 1, 2007; 170(3): 1041 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, J. Simisky, F. T. F. Tsai, and D. S. Geller A critical role of helix 3-helix 5 interaction in steroid hormone receptor function PNAS, February 22, 2005; 102(8): 2707 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Nelson, V. Prapapanich, P. E. Carrigan, P. J. Roberts, D. L. Riggs, and D. F. Smith The Heat Shock Protein 70 Cochaperone Hip Enhances Functional Maturation of Glucocorticoid Receptor Mol. Endocrinol., July 1, 2004; 18(7): 1620 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Elbi, D. A. Walker, M. Lewis, G. Romero, W. P. Sullivan, D. O. Toft, G. L. Hager, and D. B. DeFranco A Novel In Situ Assay for the Identification and Characterization of Soluble Nuclear Mobility Factors Sci. Signal., June 22, 2004; 2004(238): pl10 - pl10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kauppi, C. Jakob, M. Farnegardh, J. Yang, H. Ahola, M. Alarcon, K. Calles, O. Engstrom, J. Harlan, S. Muchmore, et al. The Three-dimensional Structures of Antagonistic and Agonistic Forms of the Glucocorticoid Receptor Ligand-binding Domain: RU-486 INDUCES A TRANSCONFORMATION THAT LEADS TO ACTIVE ANTAGONISM J. Biol. Chem., June 13, 2003; 278(25): 22748 - 22754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Keeton, T. M. Fletcher, C. T. Baumann, G. L. Hager, and C. L. Smith Glucocorticoid Receptor Domain Requirements for Chromatin Remodeling and Transcriptional Activation of the Mouse Mammary Tumor Virus Promoter in Different Nucleoprotein Contexts J. Biol. Chem., July 26, 2002; 277(31): 28247 - 28255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kucera, M. Waltner-Law, D. K. Scott, R. Prasad, and D. K. Granner A Point Mutation of the AF2 Transactivation Domain of the Glucocorticoid Receptor Disrupts Its Interaction with Steroid Receptor Coactivator 1 J. Biol. Chem., July 12, 2002; 277(29): 26098 - 26102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mackem, C. T. Baumann, and G. L. Hager A Glucocorticoid/Retinoic Acid Receptor Chimera That Displays Cytoplasmic/Nuclear Translocation in Response to Retinoic Acid. A REAL TIME SENSING ASSAY FOR NUCLEAR RECEPTOR LIGANDS J. Biol. Chem., November 30, 2001; 276(49): 45501 - 45504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Giannoukos, A. M. Silverstein, W. B. Pratt, and S. S. Simons Jr. The Seven Amino Acids (547-553) of Rat Glucocorticoid Receptor Required for Steroid and Hsp90 Binding Contain a Functionally Independent LXXLL Motif That Is Critical for Steroid Binding J. Biol. Chem., December 17, 1999; 274(51): 36527 - 36536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sheldon, C. L. Smith, J. E. Bodwell, A. U. Munck, and G. L. Hager A Ligand Binding Domain Mutation in the Mouse Glucocorticoid Receptor Functionally Links Chromatin Remodeling and Transcription Initiation Mol. Cell. Biol., December 1, 1999; 19(12): 8146 - 8157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Szapary, Y. Huang, and S. S. Simons Jr. Opposing Effects of Corepressor and Coactivators in Determining the Dose-Response Curve of Agonists, and Residual Agonist Activity of Antagonists, for Glucocorticoid Receptor-Regulated Gene Expression Mol. Endocrinol., December 1, 1999; 13(12): 2108 - 2121. [Abstract] [Full Text]
|
|
|
|
|
|
U. Lind, P. Greenidge, J.-A. Gustafsson, A. P. H. Wright, and J. Carlstedt-Duke Valine 571 Functions as a Regional Organizer in Programming the Glucocorticoid Receptor for Differential Binding of Glucocorticoids and Mineralocorticoids J. Biol. Chem., June 25, 1999; 274(26): 18515 - 18523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Smith, H. Htun, R. G. Wolford, and G. L. Hager Differential Activity of Progesterone and Glucocorticoid Receptors on Mouse Mammary Tumor Virus Templates Differing in Chromatin Structure J. Biol. Chem., May 30, 1997; 272(22): 14227 - 14235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Licitra and J. O. Liu A three-hybrid system for detecting small ligand-protein receptor interactions PNAS, November 12, 1996; 93(23): 12817 - 12821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xu, P. K. Chakraborti, M. J. Garabedian, K. R. Yamamoto, and S. S. Simons Jr. Modular Structure of Glucocorticoid Receptor Domains Is Not Equivalent to Functional Independence. STABILITY AND ACTIVITY OF THE STEROID BINDING DOMAIN ARE CONTROLLED BY SEQUENCES IN SEPARATE DOMAINS J. Biol. Chem., August 30, 1996; 271(35): 21430 - 21438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nakajima, J.-C. Hsieh, P. W. Jurutka, M. A. Galligan, C. A. Haussler, G. K. Whitfield, and M. R. Haussler Examination of the Potential Functional Role of Conserved Cysteine Residues in the Hormone Binding Domain of the Human 1,25-Dihydroxyvitamin D(3) Receptor J. Biol. Chem., March 1, 1996; 271(9): 5143 - 5149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, N. J. Sarlis, and S. S. Simons Jr. Evidence for a Common Step in Three Different Processes for Modulating the Kinetic Properties of Glucocorticoid Receptor-induced Gene Transcription J. Biol. Chem., September 22, 2000; 275(39): 30106 - 30117. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
