HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Z. G. Articles by Friedberg, E. C. Search for Related Content PubMed PubMed Citation Articles by Wang, Z. G. Articles by Friedberg, E. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 266, Issue 33, 22472-22478, 11, 1991

Nucleotide excision repair of DNA by human cell extracts is suppressed in reconstituted nucleosomes

ZG Wang, XH Wu and EC Friedberg
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.

Nucleotide excision repair is a complex biochemical process that corrects DNA damage caused by a broad spectrum of physical and chemical agents. We examined the effect of the assembly of ultraviolet- irradiated plasmid DNA into nucleosomes on nucleotide excision repair supported by human cell extracts. Repair synthesis in unassembled UV- irradiated plasmid DNA was readily detected in extracts from repair- proficient human cells. In contrast, repair synthesis was markedly suppressed in UV-irradiated DNA assembled into nucleosomes (minichromosomes). This suppression occurred at a step(s) which precedes repair synthesis during nucleotide excision repair. Human cell extracts were unable to effectively assemble plasmid DNA into nucleosomes under repair synthesis conditions. The addition of purified histones to the extracts restored their capacity for nucleosome assembly and simultaneously led to the suppression of repair synthesis. We propose that the preferential repair of actively transcribed genes relative to transcriptionally silent genes may reflect altered nucleosome conformation during transcription.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. M. Svedruzic, C. Wang, J. V. Kosmoski, and M. J. Smerdon Accommodation and Repair of a UV Photoproduct in DNA at Different Rotational Settings on the Nucleosome Surface J. Biol. Chem., December 2, 2005; 280(48): 40051 - 40057. [Abstract] [Full Text] [PDF]

				Y. Yu, Y. Teng, H. Liu, S. H. Reed, and R. Waters UV irradiation stimulates histone acetylation and chromatin remodeling at a repressed yeast locus PNAS, June 14, 2005; 102(24): 8650 - 8655. [Abstract] [Full Text] [PDF]

				B. Kysela, M. Chovanec, and P. A. Jeggo Phosphorylation of linker histones by DNA-dependent protein kinase is required for DNA ligase IV-dependent ligation in the presence of histone H1 PNAS, February 8, 2005; 102(6): 1877 - 1882. [Abstract] [Full Text] [PDF]

				H. Gaillard, D. J. Fitzgerald, C. L. Smith, C. L. Peterson, T. J. Richmond, and F. Thoma Chromatin Remodeling Activities Act on UV-damaged Nucleosomes and Modulate DNA Damage Accessibility to Photolyase J. Biol. Chem., May 9, 2003; 278(20): 17655 - 17663. [Abstract] [Full Text] [PDF]

				R. Hara and A. Sancar The SWI/SNF Chromatin-Remodeling Factor Stimulates Repair by Human Excision Nuclease in the Mononucleosome Core Particle Mol. Cell. Biol., October 1, 2002; 22(19): 6779 - 6787. [Abstract] [Full Text] [PDF]

				R. Hara, J. Mo, and A. Sancar DNA Damage in the Nucleosome Core Is Refractory to Repair by Human Excision Nuclease Mol. Cell. Biol., December 15, 2000; 20(24): 9173 - 9181. [Abstract] [Full Text]

				B. C. McKay, M. Ljungman, and A. J. Rainbow1 Potential roles for p53 in nucleotide excision repair Carcinogenesis, August 1, 1999; 20(8): 1389 - 1396. [Abstract] [Full Text] [PDF]

				C. P. Selby and A. Sancar Cockayne syndrome group B protein enhances elongation by RNA polymerase II PNAS, October 14, 1997; 94(21): 11205 - 11209. [Abstract] [Full Text] [PDF]

				Y. Tu, S. Bates, and G. P. Pfeifer Sequence-specific and Domain-specific DNA Repair in Xeroderma Pigmentosum and Cockayne Syndrome Cells J. Biol. Chem., August 15, 1997; 272(33): 20747 - 20755. [Abstract] [Full Text] [PDF]

				X. Liu and M. J. Smerdon Nucleotide Excision Repair of the 5 S Ribosomal RNA Gene Assembled into a Nucleosome J. Biol. Chem., July 28, 2000; 275(31): 23729 - 23735. [Abstract] [Full Text] [PDF]