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J. Biol. Chem., Vol. 266, Issue 34, 23268-23273, 12, 1991
KE Brown, R Lawrence and GE Sonenshein
We have isolated a partial cDNA for alpha 1(XI) collagen from a bovine
smooth muscle cell (SMC) library. Previously, this collagen was not known
to be expressed in SMCs. Comparison of the nucleotide and deduced amino
acid sequence of the 2.7-kilobase bovine clone and the human alpha 1(XI)
sequence indicates 92 and 98% homology, respectively. Bovine SMCs in
culture were found to produce alpha 1(XI) mRNA. However, alpha 2(XI) and
alpha 1(II) collagen RNA were not detectable; therefore, SMCs cannot
synthesize the same type XI collagen as found in cartilage. Since type XI
collagen is structurally related to type V collagen, the expression of
alpha 1(XI) and alpha 2(V) collagen mRNA in SMCs was characterized. Levels
of alpha 1(XI) and alpha 2(V) collagen mRNAs were low in exponentially
growing SMCs and increased 3-4-fold as cells became confluent. Increased
mRNA levels were also observed when exponentially growing subconfluent SMCs
were incubated in medium containing 0.5% fetal bovine serum for 24 h,
similar to the effects of serum deprivation on the expression of types I
and III collagen genes (Kindy, M. S., Chang, C.-J., and Sonenshein, G. E.
(1988) J. Biol. Chem. 263, 11426-11430). However, as cell density
increased, serum deprivation resulted in very different responses for these
collagen genes. Serum deprivation caused a decrease in expression of alpha
1(XI) and alpha 2(V) collagen mRNAs in cultures as they approached
confluence. In contrast, at confluence alpha 1(I) and alpha 2(I) mRNA
levels no longer responded to serum concentration whereas expression of
alpha 1(III) mRNA remained inducible by serum deprivation. These results
suggest concerted regulation of alpha 1(XI) and alpha 2(V) collagen gene
expression, which is distinct from that for the chains of type I and type
III collagen with respect to cell density and serum.
Concerted modulation of alpha 1(XI) and alpha 2(V) collagen mRNAs in bovine vascular smooth muscle cells
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
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