| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 34, 23399-23406, 12, 1991
PW Park, DD Roberts, LE Grosso, WC Parks, J Rosenbloom, WR Abrams and RP Mecham
Many pathogenic bacteria specifically bind to components of the
extracellular matrix. In this study, we report the specific association of
Staphylococcus aureus with elastin, a major structural component of elastic
tissue. Competition assays in which the binding of radiolabeled
tropoelastin was inhibited by excess unlabeled elastin peptides, but not by
other proteins, established the specificity of the interaction. Kinetic
studies showed that tropoelastin binding to the bacteria was rapid and
saturable. Scatchard analysis of the equilibrium binding data indicated the
presence of a single class of high affinity binding sites (KD approximately
4-7 nM) with approximately 1000 sites per organism. Protease susceptibility
suggested that the elastin binding moiety on S. aureus was a protein, which
was confirmed by the isolation of a 25-kDa elastin-binding protein from S.
aureus extracts through affinity chromatography. Using a truncated form of
tropoelastin, the bacterial binding domain on elastin was mapped to a
30-kDa fragment at the amino end of the molecule. Although the precise
amino acid sequence recognized by the staphylococcal elastin receptor has
not been characterized, it is clearly different from the region of
tropoelastin that specifies binding to mammalian elastin receptors.
Binding of elastin to Staphylococcus aureus
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Nakakido, Y. Tanaka, and K. Tsumoto The N-terminal domain of elastin-binding protein of Staphylococcus aureus changes its secondary structure in a membrane-mimetic environment J. Biochem., August 1, 2007; 142(2): 131 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sakinc, B. Kleine, and S. G. Gatermann SdrI, a Serine-Aspartate Repeat Protein Identified in Staphylococcus saprophyticus Strain 7108, Is a Collagen-Binding Protein. Infect. Immun., August 1, 2006; 74(8): 4615 - 4623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Thomassin, C. C. Werneck, T. J. Broekelmann, C. Gleyzal, I. K. Hornstra, R. P. Mecham, and P. Sommer The Pro-regions of Lysyl Oxidase and Lysyl Oxidase-like 1 Are Required for Deposition onto Elastic Fibers J. Biol. Chem., December 30, 2005; 280(52): 42848 - 42855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Broekelmann, B. A. Kozel, H. Ishibashi, C. C. Werneck, F. W. Keeley, L. Zhang, and R. P. Mecham Tropoelastin Interacts with Cell-surface Glycosaminoglycans via Its COOH-terminal Domain J. Biol. Chem., December 9, 2005; 280(49): 40939 - 40947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Roche, R. Downer, F. Keane, P. Speziale, P. W. Park, and T. J. Foster The N-terminal A Domain of Fibronectin-binding Proteins A and B Promotes Adhesion of Staphylococcus aureus to Elastin J. Biol. Chem., September 10, 2004; 279(37): 38433 - 38440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vaudaux, P. Francois, C. Bisognano, W. L. Kelley, D. P. Lew, J. Schrenzel, R. A. Proctor, P. J. McNamara, G. Peters, and C. Von Eiff Increased Expression of Clumping Factor and Fibronectin-Binding Proteins by hemB Mutants of Staphylococcus aureus Expressing Small Colony Variant Phenotypes Infect. Immun., October 1, 2002; 70(10): 5428 - 5437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Savolainen, L. Paulin, B. Westerlund-Wikstrom, T. J. Foster, T. K. Korhonen, and P. Kuusela Expression of pls, a Gene Closely Associated with the mecA Gene of Methicillin-Resistant Staphylococcus aureus, Prevents Bacterial Adhesion In Vitro Infect. Immun., May 1, 2001; 69(5): 3013 - 3020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mongodin, O. Bajolet, J. Hinnrasky, E. Puchelle, and S. de Bentzmann Cell Wall-associated Protein A as a Tool for Immunolocalization of Staphylococcus aureus in Infected Human Airway Epithelium J. Histochem. Cytochem., April 1, 2000; 48(4): 523 - 534. [Abstract] [Full Text]
|
|
|
|
|
|
N. Mohamed, M. A. Teeters, J. M. Patti, M. Hook, and J. M. Ross Inhibition of Staphylococcus aureus Adherence to Collagen under Dynamic Conditions Infect. Immun., February 1, 1999; 67(2): 589 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Park, T. J. Broekelmann, B. R. Mecham, and R. P. Mecham Characterization of the Elastin Binding Domain in the Cell-surface 25-kDa Elastin-binding Protein of Staphylococcus aureus (EbpS) J. Biol. Chem., January 29, 1999; 274(5): 2845 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. van Belkum, S. Scherer, L. van Alphen, and H. Verbrugh Short-Sequence DNA Repeats in Prokaryotic Genomes Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 275 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yan, R. G. Rodrigues, D. Cahn-Hidalgo, T. J. Walsh, and D. D. Roberts Hemoglobin Induces Binding of Several Extracellular Matrix Proteins to Candida albicans. IDENTIFICATION OF A COMMON RECEPTOR FOR FIBRONECTIN, FIBRINOGEN, AND LAMININ J. Biol. Chem., March 6, 1998; 273(10): 5638 - 5644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Vaudaux, V. Monzillo, P. Francois, D. P. Lew, T. J. Foster, and B. Berger-Bächi Introduction of the mec Element (Methicillin Resistance) into Staphylococcus aureus Alters In Vitro Functional Activities of Fibrinogen and Fibronectin Adhesins Antimicrob. Agents Chemother., March 1, 1998; 42(3): 564 - 570. [Abstract] [Full Text]
|
|
|
|
|
|
P. W. Park, J. Rosenbloom, W. R. Abrams, J. Rosenbloom, and R. P. Mecham Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus J. Biol. Chem., June 28, 1996; 271(26): 15803 - 15809. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
