J. Biol. Chem., Vol. 266, Issue 35, 23872-23877, 12, 1991
A single base pair deletion from the inactive octamer-like motif of the 7S K distal sequence element brings full functionality in vivo
H Kleinert, R Assert and BJ Benecke
Department of Biochemistry, Faculty of Chemistry, Ruhr-University, Bochum, Federal Republic of Germany.
Octamer sequence elements were analyzed for their capacity to induce the 7S
K "core" promoter in vivo. The U6 distal sequence element (DSE) which
contains a consensus sequence octamer, was able to support efficient 7S K
expression in vivo. In contrast, no such function could be attributed to
the octamer-like element alone, which is present within the 7S K DSE.
However, conversion of this octamer-like element (ATTTaGCAT) to the octamer
consensus sequence ATTTGCAT generated a potent DSE, even in the absence of
the CACCC box, which constitutes the major functional element of the 7S K
DSE. Both the consensus and the octamer-like sequences revealed no
cooperativity with the CACCC box. Together, these results demonstrate that
the octamer-like element of the wild-type 7S K DSE is definitely not
functional in vivo. Furthermore, our experiments indicate that in contrast
to the RNA polymerase II-transcribed small nuclear RNA genes, in intact
cells a single functional DSE motif is necessary and sufficient for maximal
transcription by RNA polymerase III of the 7S K RNA gene.
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