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J. Biol. Chem., Vol. 266, Issue 35, 23932-23935, 12, 1991
S Borukhov, J Lee and A Goldfarb
Stalled elongation complexes of Escherichia coli RNA polymerase were
prepared carrying the photo-cross-linkable 8-azido derivative of adenine at
the 3'-terminus of the nascent RNA chain. Ultraviolet irradiation of such
complexes resulted in the cross-linking of radiolabeled RNA exclusively to
the beta' subunit of RNA polymerase. The adduct was mapped between Met932
and Trp1020 in the linear sequence of the beta' polypeptide using specific
chemical degradation of the cross-linked species.
Mapping of a contact for the RNA 3' terminus in the largest subunit of RNA polymerase
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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