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J. Biol. Chem., Vol. 266, Issue 9, 5955-5962, 03, 1991
JE Chatterton, ML Phillips, LK Curtiss, RW Milne, YL Marcel and VN Schumaker
Apolipoprotein B (apoB) was mapped using electron microscopy to visualize
pairs of monoclonal antibodies binding to the low density lipoprotein (LDL)
surface. The sites at which these monoclonals bind the apoB polypeptide
sequence had already been established. The angular distances between all
possible pairs of binding sites except one allowed the relative placement
of six epitopes on the LDL sphere. We conclude that apoB extends over at
least a hemisphere of the LDL surface since four epitopes are located in
the Northern Hemisphere at sites arbitrarily designated as the North Pole,
the Aleutian Islands, Bogota, and in the Atlantic Ocean, while two are
found in the Southern Hemisphere at Buenos Aires and at Madagascar. ApoB
appears to possess a restricted flexibility, since these relative epitope
locations show a substantial standard deviation in latitude and longitude.
Mapping of additional epitopes may provide an answer to the question of
whether apoB circumnavigates the LDL sphere.
Mapping apolipoprotein B on the low density lipoprotein surface by immunoelectron microscopy
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024-1569.
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