HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Benhamou, M. Articles by Siraganian, R. P. Search for Related Content PubMed PubMed Citation Articles by Benhamou, M. Articles by Siraganian, R. P. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 267, Issue 11, 7310-7314, Apr, 1992

High-affinity IgE receptor-mediated stimulation of rat basophilic leukemia (RBL-2H3) cells induces early and late protein-tyrosine phosphorylations

M Benhamou, V Stephan, KC Robbins and RP Siraganian
Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.

We reported previously that stimulation of RBL-2H3 cells through the high-affinity IgE receptor resulted in tyrosine phosphorylation of a 72- kDa protein (pp72) that was coupled to signal transduction. In the present study, although pp72 tyrosine phosphorylation was induced only by antigen triggering, stimulation of RBL-2H3 cells by either antigen or the calcium-ionophore A23187 led to increased tyrosine phosphorylation of a 110-kDa protein (pp110). This tyrosine phosphorylated protein was also observed when RBL-2H3 cells were transfected with the G protein-coupled m3 muscarinic receptor and then stimulated to secrete with carbachol. In contrast to tyrosine phosphorylation of pp72, antigen-induced pp110 tyrosine phosphorylation required extracellular calcium, was absent in cells depleted of protein kinase C, and was detected between 1 and 5 min after stimulation. The protein-tyrosine kinase inhibitor genistein blocked both histamine release and tyrosine phosphorylation induced by A23187. Altogether, the data suggest a role for pp110 in secretion. However, protein kinase C activation induced pp110 tyrosine phosphorylation but not histamine release demonstrating that pp110 tyrosine phosphorylation alone is not sufficient for degranulation. We conclude that tyrosine phosphorylation of pp72 is associated with the early steps of IgE receptor-generated signaling, whereas pp110 tyrosine phosphorylation occurs secondary to calcium influx and protein kinase C activation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				O. Amir-Moazami, C. Alexia, N. Charles, P. Launay, R. C. Monteiro, and M. Benhamou Phospholipid Scramblase 1 Modulates a Selected Set of IgE Receptor-mediated Mast Cell Responses through LAT-dependent Pathway J. Biol. Chem., September 12, 2008; 283(37): 25514 - 25523. [Abstract] [Full Text] [PDF]

				C. Tkaczyk, M. A. Beaven, S. M. Brachman, D. D. Metcalfe, and A. M. Gilfillan The Phospholipase C{gamma}1-dependent Pathway of Fc{epsilon}RI-mediated Mast Cell Activation Is Regulated Independently of Phosphatidylinositol 3-Kinase J. Biol. Chem., November 28, 2003; 278(48): 48474 - 48484. [Abstract] [Full Text] [PDF]

				L. Geng, S. Pfister, S.-K. Kraeft, and C. E. Rudd Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: Differential involvement of the FYB SH3 domain PNAS, September 5, 2001; (2001) 191378198. [Abstract] [Full Text] [PDF]

				D. S. Cissel, P. F. Fraundorfer, and M. A. Beaven Thapsigargin-Induced Secretion Is Dependent on Activation of a Cholera Toxin-Sensitive and Phosphatidylinositol-3-kinase-Regulated Phospholipase D in a Mast Cell Line J. Pharmacol. Exp. Ther., April 1, 1998; 285(1): 110 - 118. [Abstract] [Full Text]

				H. Okazaki, J. Zhang, M. M. Hamawy, and R. P. Siraganian Activation of Protein-tyrosine Kinase Pyk2 Is Downstream of Syk in Fcepsilon RI Signaling J. Biol. Chem., December 19, 1997; 272(51): 32443 - 32447. [Abstract] [Full Text] [PDF]

				M. M. Hamawy, M. Swieter, S. E. Mergenhagen, and R. P. Siraganian Reconstitution of High Affinity IgE Receptor-mediated Secretion By Transfecting Protein Tyrosine Kinase pp125FAK J. Biol. Chem., November 28, 1997; 272(48): 30498 - 30503. [Abstract] [Full Text] [PDF]

				T. Kimura, H. Sakamoto, E. Appella, and R. P. Siraganian The Negative Signaling Molecule SH2 Domain-containing Inositol-Polyphosphate 5-Phosphatase (SHIP) Binds to the Tyrosine-phosphorylated beta  Subunit of the High Affinity IgE Receptor J. Biol. Chem., May 23, 1997; 272(21): 13991 - 13996. [Abstract] [Full Text] [PDF]

				R. Paolini, A. Serra, and J.-P. Kinet Persistence of Tyrosine-phosphorylated Fcepsilon RI in Deactivated Cells J. Biol. Chem., July 5, 1996; 271(27): 15987 - 15992. [Abstract] [Full Text] [PDF]

				B. Jabril-Cuenod, C. Zhang, A. M. Scharenberg, R. Paolini, R. Numerof, M. A. Beaven, and J.-P. Kinet Syk-dependent Phosphorylation of Shc. A POTENTIAL LINK BETWEEN Fc&egr;RI AND THE Ras/MITOGEN-ACTIVATED PROTEIN KINASE SIGNALING PATHWAY THROUGH SOS AND Grb2 J. Biol. Chem., July 5, 1996; 271(27): 16268 - 16272. [Abstract] [Full Text] [PDF]

				P. C. Wolfe, E.-Y. Chang, J. Rivera, and C. Fewtrell Differential Effects of the Protein Kinase C Activator Phorbol 12-Myristate 13-Acetate on Calcium Responses and Secretion in Adherent and Suspended RBL-2H3 Mucosal Mast Cells J. Biol. Chem., March 22, 1996; 271(12): 6658 - 6665. [Abstract] [Full Text] [PDF]

				R. Seger, Y. Biener, R. Feinstein, T. Hanoch, A. Gazit, and Y. Zick Differential Activation of Mitogen-activated Protein Kinase and S6 Kinase Signaling Pathways by 12-O-Tetradecanoylphorbol-13-acetate (TPA) and Insulin J. Biol. Chem., November 24, 1995; 270(47): 28325 - 28330. [Abstract] [Full Text] [PDF]

				M. Swieter, E. H. Berenstein, W. D. Swaim, and R. P. Siraganian Aggregation of IgE Receptors in Rat Basophilic Leukemia 2H3 Cells Induces Tyrosine Phosphorylation of the Cytosolic Protein-tyrosine Phosphatase HePTP J. Biol. Chem., September 15, 1995; 270(37): 21902 - 21906. [Abstract] [Full Text] [PDF]

				M. M. Hamawy, K. Minoguchi, W. D. Swaim, S. E. Mergenhagen, and R. P. Siraganian A 77-kDa Protein Associates with pp125[IMAGE] in Mast Cells and Becomes Tyrosine-phosphorylated by High Affinity IgE Receptor Aggregation J. Biol. Chem., May 19, 1995; 270(20): 12305 - 12309. [Abstract] [Full Text] [PDF]

				N. Hirasawa, A. Scharenberg, H. Yamamura, M. A. Beaven, and J.-P. Kinet A Requirement for Syk in the Activation of the Microtubule-associated Protein Kinase/Phospholipase A[IMAGE] Pathway by Fc[IMAGE]R1 Is Not Shared by a G Protein-coupled Receptor J. Biol. Chem., May 5, 1995; 270(18): 10960 - 10967. [Abstract] [Full Text] [PDF]

				L. Geng, S. Pfister, S.-K. Kraeft, and C. E. Rudd Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: Differential involvement of the FYB SH3 domain PNAS, September 25, 2001; 98(20): 11527 - 11532. [Abstract] [Full Text] [PDF]