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J. Biol. Chem., Vol. 267, Issue 20, 13884-13889, Jul, 1992
DC Zovich, A Orologa, M Okuno, LW Kong, DA Talmage, R Piantedosi, DS Goodman and WS Blaner
Recently, we demonstrated that adipose tissue plays an important role in
retinol storage and retinol-binding protein (RBP) synthesis. Our data
suggested that RBP expression in adipose tissue is dependent on the state
of adipocyte differentiation. To examine this possibility, we explored the
differentiation-dependent expression of RBP using BFC-1 beta preadipocytes,
which can be stimulated to undergo adipose differentiation. Total RNA was
isolated from undifferentiated (preadipocytes) and differentiated
(adipocytes) BFC-1 beta cells and analyzed by Northern blotting. RBP mRNA
was not detected in the preadipocytes, but considerable RBP mRNA was
present in differentiated BFC-1 beta cells. In BFC-1 beta cells, induced to
differentiate with insulin and thyroid hormone, RBP mRNA was first detected
after 4 days, reached a maximum level by day 10, and remained at this
maximum level for at least 2 more days. Cellular retinol-binding protein
was expressed at low levels in the BFC-1 beta preadipocytes and the level
of expression increased for 6 days after induction to differentiate and
slowly declined on later days. Neither the maximum level of RBP expression
nor the day on which this level was reached was influenced by the level of
retinol provided in the BFC-1 beta culture medium. BFC- 1 beta cells
secreted newly synthesized RBP into the culture medium at a rate of 43 +/-
14 ng RBP/24 h/10(6) adipocytes. When the BFC-1 beta adipocytes were
provided 1.0 microM retinol in the medium, they accumulated the retinol and
synthesized retinyl esters. These studies with BFC-1 beta cells confirm
that RBP synthesis and secretion and retinol accumulation are intrinsic
properties of differentiated adipocytes. Furthermore, they suggest that RBP
and cellular retinol- binding protein gene expression are regulated as part
of a package of genes which are modulated during adipocyte differentiation.
Differentiation-dependent expression of retinoid-binding proteins in BFC-1 beta adipocytes
Institute of Human Nutrition, Columbia University, New York, New York 10032.
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