J. Biol. Chem., Vol. 267, Issue 33, 23451-23454, 11, 1992
Interleukin-1 alpha induces the accumulation of cytosolic phospholipase A2 and the release of prostaglandin E2 in human fibroblasts
LL Lin, AY Lin and DL DeWitt
Genetics Institute, Inc., Cambridge, Massachusetts 02140.
Treatment of the human lung fibroblast cell line, WI-38, with interleukin-1
alpha (IL-1 alpha) results in a large increase in the production of
cytosolic phospholipase A2 (cPLA2) and prostaglandin E2 (PGE2). The
IL-1-induced accumulation of cPLA2 is closely correlated with increased
PGE2 release. In contrast to cPLA2, the level of cyclooxygenase remains
unchanged following IL-1 alpha treatment. The glucocorticoid,
dexamethasone, blocks the IL-1 alpha-mediated increases in both cPLA2 and
PGE2 without affecting the cyclooxygenase level. Taken together, these data
suggest that in these cells, the regulation of prostaglandin production by
IL-1 and glucocorticoid can be attributed to the level of cPLA2. These
results provide a new mechanism for the effect of IL-1 and glucocorticoids
on eicosanoid synthesis and provide additional support for an important
role of cPLA2 in the inflammatory response.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
