| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 16, 11663-11669, 06, 1993
MI Aveldano, BS Robinson, DW Johnson and A Poulos
The formation of long and very long chain (VLC) n-6 polyunsaturated fatty
acids (PUFA) in isolated rat seminiferous tubules was investigated by
following the metabolism of three 1-14C-labeled n-6 tetraenoic fatty acids
(20:4, 24:4, and 32:4) and [U-14C]acetate. In contrast to [14C]32:4, which
was poorly incorporated and altered, [14C]20:4 and [14C]24:4 were
efficiently taken up by the tubules, esterified into lipids, elongated to
VLCPUFA, and desaturated to pentaenoic fatty acids; the rate of [14C]24:4
desaturation to [14C]24:5 was notably high. The main products with
[14C]acetate as precursor were labeled saturates and VLCPUFA, most of the
label in tetraenoic and pentaenoic acids appearing in 24:4 and 24:5,
respectively. These two C24 polyenes, connected by a delta 6 desaturation,
may play a central role in n-6 PUFA metabolism, in their capacity as
potential precursors of longer polyenes via elongation and of shorter ones,
such as 22:5n-6, via retroconversion. Triacylglycerols, rich in C22 and C24
polyenes, incorporated the greatest amounts of both [14C] acetate-derived
and exogenous 14C-PUFA, suggesting that this lipid class is involved in the
traffic and metabolism of testicular PUFA. The detection of a series of
unusual odd-chain tetraenoic and pentaenoic acids, also labeled with [U-
14C]acetate, suggests that a PUFA chain shortening mechanism occurs in
testis involving alpha- in addition to beta-oxidation. We speculate that
alpha-oxidation plays a role in the retroconversion of PUFA.
Long and very long chain polyunsaturated fatty acids of the n-6 series in rat seminiferous tubules. Active desaturation of 24:4n-6 to 24:5n-6 and concomitant formation of odd and even chain tetraenoic and pentaenoic fatty acids up to C32
Department of Chemical Pathology, Adelaide Children's Hospital, South Australia.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-P. Agbaga, R. S. Brush, M. N. A. Mandal, K. Henry, M. H. Elliott, and R. E. Anderson Role of Stargardt-3 macular dystrophy protein (ELOVL4) in the biosynthesis of very long chain fatty acids PNAS, September 2, 2008; 105(35): 12843 - 12848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nenicu, G. H Luers, W. Kovacs, M. Bergmann, and E. Baumgart-Vogt Peroxisomes in Human and Mouse Testis: Differential Expression of Peroxisomal Proteins in Germ Cells and Distinct Somatic Cell Types of the Testis Biol Reprod, December 1, 2007; 77(6): 1060 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Zanetti, E. N. Maldonado, and M. I. Aveldano Doxorubicin Affects Testicular Lipids with Long-Chain (C18-C22) and Very Long-Chain (C24-C32) Polyunsaturated Fatty Acids Cancer Res., July 15, 2007; 67(14): 6973 - 6980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Furland, E. N. Maldonado, P. A. Aresti, and M. I. Aveldano Changes in Lipids Containing Long- and Very Long-Chain Polyunsaturated Fatty Acids in Cryptorchid Rat Testes Biol Reprod, July 1, 2007; 77(1): 181 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Furland, S. R. Zanetti, G. M. Oresti, E. N. Maldonado, and M. I. Aveldano Ceramides and Sphingomyelins with High Proportions of Very Long-chain Polyunsaturated Fatty acids in Mammalian Germ Cells J. Biol. Chem., June 22, 2007; 282(25): 18141 - 18150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sandhoff, R. Geyer, R. Jennemann, C. Paret, E. Kiss, T. Yamashita, K. Gorgas, T. P. Sijmonsma, M. Iwamori, C. Finaz, et al. Novel Class of Glycosphingolipids Involved in Male Fertility J. Biol. Chem., July 22, 2005; 280(29): 27310 - 27318. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
