HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, W. Articles by Lim, L. Search for Related Content PubMed PubMed Citation Articles by Chen, W. Articles by Lim, L. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 268, Issue 18, 13280-13285, Jun, 1993

The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation

W Chen, HH Lim and L Lim
Institute of Molecular and Cell Biology, National University of Singapore.

A Caenorhabditis elegans cDNA encoding a homologue of the p21 ras- related CDC42, designated as CDC42Ce, was isolated from a nematode mixed stage cDNA library. The encoded protein of 188 amino acid residues has 85% identity to both human G25K and CDC42Hs and 79 and 76% identity to the yeast CDC42Sp and CDC42Sc proteins, respectively. The CDC42Ce cDNA maps to a position on C. elegans chromosome II in close proximity to lin-26, a cell lineage gene. The CDC42Ce cDNA hybridizes to 2- and 1.5-kilobase mRNAs. Their expression is developmentally regulated with highest levels at the embryonic stage, decreasing progressively during development except for an increase of the more abundant 1.5-kilobase mRNA at the L3 stage. The glutathione S- transferase/CDC42Ce fusion protein expressed in Escherichia coli displays both GTP binding and intrinsic GTPase activities. The GTPase activity of CDC42Ce is moderately stimulated by human n-chimaerin, a GTPase-activating protein for the related p21 rac1. The CDC42Ce protein complements the temperature-sensitive lethal mutation cdc42-1 in yeast Saccharomyces cerevisiae. These data suggest that CDC42Ce is the C. elegans homologue of the yeast CDC42. The developmental expression pattern of mRNA and is biochemical properties of its encoded protein which are closely related to CErac1 suggest that the two p21s might be involved in related biological processes.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Bassilana and R. A. Arkowitz Rac1 and Cdc42 Have Different Roles in Candida albicans Development Eukaryot. Cell, February 1, 2006; 5(2): 321 - 329. [Abstract] [Full Text] [PDF]

				J. Scheffer, C. Chen, P. Heidrich, M. B. Dickman, and P. Tudzynski A CDC42 Homologue in Claviceps purpurea Is Involved in Vegetative Differentiation and Is Essential for Pathogenicity Eukaryot. Cell, July 1, 2005; 4(7): 1228 - 1238. [Abstract] [Full Text] [PDF]

				T. J. Kubiseski, J. Culotti, and T. Pawson Functional Analysis of the Caenorhabditis elegans UNC-73B PH Domain Demonstrates a Role in Activation of the Rac GTPase In Vitro and Axon Guidance In Vivo Mol. Cell. Biol., October 1, 2003; 23(19): 6823 - 6835. [Abstract] [Full Text] [PDF]

				K. J. Boyce, M. J. Hynes, and A. Andrianopoulos Control of morphogenesis and actin localization by the Penicillium marneffei RAC homolog J. Cell Sci., April 1, 2003; 116(7): 1249 - 1260. [Abstract] [Full Text] [PDF]

				C. A. R. Hurtado, J.-M. Beckerich, C. Gaillardin, and R. A. Rachubinski A Rac Homolog Is Required for Induction of Hyphal Growth in the Dimorphic Yeast Yarrowia lipolytica J. Bacteriol., May 1, 2000; 182(9): 2376 - 2386. [Abstract] [Full Text]

				D. I. Johnson Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105. [Abstract] [Full Text] [PDF]

				B. Zhang, Y. Zhang, C. C. Collins, D. I. Johnson, and Y. Zheng A Built-in Arginine Finger Triggers the Self-stimulatory GTPase-activating Activity of Rho Family GTPases J. Biol. Chem., January 29, 1999; 274(5): 2609 - 2612. [Abstract] [Full Text] [PDF]

				P. Yang, S. Kansra, R. A. Pimental, M. Gilbreth, and S. Marcus Cloning and Characterization of shk2, a Gene Encoding a Novel p21-activated Protein Kinase from Fission Yeast J. Biol. Chem., July 17, 1998; 273(29): 18481 - 18489. [Abstract] [Full Text] [PDF]

				C. R. Davis, T. J. Richman, S. B. Deliduka, J. O. Blaisdell, C. C. Collins, and D. I. Johnson Analysis of the Mechanisms of Action of the Saccharomyces cerevisiae Dominant Lethal cdc42G12V and Dominant Negative cdc42D118A Mutations J. Biol. Chem., January 9, 1998; 273(2): 849 - 858. [Abstract] [Full Text] [PDF]

				A Wissmann, J Ingles, J D McGhee, and P E Mains Caenorhabditis elegans LET-502 is related to Rho-binding kinases and human myotonic dystrophy kinase and interacts genetically with a homolog of the regulatory subunit of smooth muscle myosin phosphatase to affect cell shape. Genes & Dev., February 15, 1997; 11(4): 409 - 422. [Abstract] [PDF]

				W. Chen, S. Chen, S. F. Yap, and L. Lim The Caenorhabditis elegans p21-activated Kinase (CePAK) Colocalizes with CeRac1 and CDC42Ce at Hypodermal Cell Boundaries during Embryo Elongation J. Biol. Chem., October 18, 1996; 271(42): 26362 - 26368. [Abstract] [Full Text] [PDF]

				T Roemer, K Madden, J Chang, and M Snyder Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein. Genes & Dev., April 1, 1996; 10(7): 777 - 793. [Abstract] [PDF]