| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 18, 13454-13461, 06, 1993
F Simonin, L Hofferer, PL Panzeter, S Muller, G de Murcia and FR Althaus
The cDNA encoding the carboxyl-terminal 40-kDa domain of human poly(ADP-
ribose) polymerase was inserted into an expression vector. The recombinant
protein was overproduced in Escherichia coli, and purified to homogeneity.
The 40-kDa domain had the same affinity (Km) for NAD+ as the full-length
enzyme, expressed abortive NAD+ glycohydrolase activity, catalyzed the
initiation, elongation, and branching of ADP- ribose polymers, but
exhibited no DNA dependence. Its specific activity was approximately
500-fold lower than that of the whole enzyme activated by DNA strand
breaks. Surprisingly, the carboxyl-terminal 40- kDa domain exhibited the
processive mode of polymer attachment typical of full-length
poly(ADP-ribose) polymerase and was able to modify histones H1 and H2B.
Finally, the polymer sizes formed by the 40-kDa domain were influenced by
histone H1.
The carboxyl-terminal domain of human poly(ADP-ribose) polymerase. Overproduction in Escherichia coli, large scale purification, and characterization
Institut de Biologie Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. R. Plummer and H. Calvert Targeting Poly(ADP-Ribose) Polymerase: A Two-Armed Strategy for Cancer Therapy Am. Assoc. Cancer Res. Educ. Book, April 12, 2008; 2008(1): 15 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Niere, S. Kernstock, F. Koch-Nolte, and M. Ziegler Functional Localization of Two Poly(ADP-Ribose)-Degrading Enzymes to the Mitochondrial Matrix Mol. Cell. Biol., January 15, 2008; 28(2): 814 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Plummer and H. Calvert Targeting Poly(ADP-Ribose) Polymerase: A Two-Armed Strategy for Cancer Therapy Clin. Cancer Res., November 1, 2007; 13(21): 6252 - 6256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Nguewa, M. A. Fuertes, C. Alonso, and J. M. Perez Pharmacological Modulation of Poly(ADP-ribose) Polymerase-Mediated Cell Death: Exploitation in Cancer Chemotherapy Mol. Pharmacol., November 1, 2003; 64(5): 1007 - 1014. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wieler, J.-P. Gagne, H. Vaziri, G. G. Poirier, and S. Benchimol Poly(ADP-ribose) Polymerase-1 Is a Positive Regulator of the p53-mediated G1 Arrest Response following Ionizing Radiation J. Biol. Chem., May 23, 2003; 278(21): 18914 - 18921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Sallmann, M. D. Vodenicharov, Z.-Q. Wang, and G. G. Poirier Characterization of sPARP-1. AN ALTERNATIVE PRODUCT OF PARP-1 GENE WITH POLY(ADP-RIBOSE) POLYMERASE ACTIVITY INDEPENDENT OF DNA STRAND BREAKS J. Biol. Chem., May 12, 2000; 275(20): 15504 - 15511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Kickhoefer, A. C. Siva, N. L. Kedersha, E. M. Inman, C. Ruland, M. Streuli, and L. H. Rome The 193-kD Vault Protein, VPARP, Is a Novel Poly(ADP-ribose) Polymerase J. Cell Biol., September 6, 1999; 146(5): 917 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. T. Marsischky, B. A. Wilson, and R. J. Collier Role of Glutamic Acid 988 of Human Poly-ADP-ribose Polymerase in Polymer Formation J. Biol. Chem., February 17, 1995; 270(7): 3247 - 3254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Buki, P. I. Bauer, A. Hakam, and E. Kun Identification of Domains of Poly(ADP-ribose) Polymerase for Protein Binding and Self-association J. Biol. Chem., February 17, 1995; 270(7): 3370 - 3377. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
