| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 2, 961-967, 01, 1993
R Hirata, N Umemoto, MN Ho, Y Ohya, TH Stevens and Y Anraku
vma12 mutants of the yeast Saccharomyces cerevisiae, which were originally
identified as calcium-sensitive (cls) mutants that were also respiratory
deficient (Pet-), have a defect in vacuolar membrane H(+)- ATPase activity
(Ohya, Y., Umemoto, N., Tanida, I., Ohta, A., Iida, H., and Anraku, Y.
(1991) J. Biol. Chem. 266, 13971-13977). The VMA12 gene was cloned by
complementation of the growth defects of vma12 mutants. The nucleotide
sequence of the gene predicts a polypeptide of 215 amino acids (25.2 kDa)
with two putative membrane-spanning domains. A null vma12 mutant,
constructed by chromosomal deletion of the gene, is viable but has
completely lost the vacuolar membrane H(+)-ATPase activity and exhibits the
same growth defects as observed for the original vma12 mutants. Synthesis
and targeting of the subunits of the H(+)-ATPase in the delta vma12 mutant
cells were examined by Western blotting analyses of whole cell and vacuolar
membrane protein extracts. None of the peripheral membrane subunits that we
analyzed (the 69-, 60- , 42-, and 27-kDa subunits) was detected in the
vacuolar membrane fractions, although the cellular levels of these
polypeptides appeared to be normal. The 100- and 17-kDa integral membrane
subunits of the enzyme were absent or present at a substantially reduced
level in mutant vacuolar membrane fractions. Anti-Vma12p antibodies
recognized a vacuolar protein with the expected molecular mass of 25 kDa.
However, the Vma12 protein was not detected in the vacuolar membrane ATPase
complex that had been solubilized with a zwitterionic detergent, ZW3- 14,
and purified by glycerol gradient centrifugation (Kane, P. M., Yamashiro,
C. T., and Stevens, T. H. (1989) J. Biol. Chem. 264, 19236- 19244). These
results indicate that the VMA12 gene product is not a component of the
active vacuolar ATPase complex and instead suggest that this protein is
required during the process of assembly and/or targeting of the enzyme
complex to the vacuolar membrane.
VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae
Department of Biology, Faculty of Science, University of Tokyo, Japan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Ohnuki, S. Nogami, H. Kanai, D. Hirata, Y. Nakatani, S. Morishita, and Y. Ohya Diversity of Ca2+-Induced Morphology Revealed by Morphological Phenotyping of Ca2+-Sensitive Mutants of Saccharomyces cerevisiae Eukaryot. Cell, May 1, 2007; 6(5): 817 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Davis-Kaplan, M. A. Compton, A. R. Flannery, D. M. Ward, J. Kaplan, T. H. Stevens, and L. A. Graham PKR1 Encodes an Assembly Factor for the Yeast V-Type ATPase J. Biol. Chem., October 20, 2006; 281(42): 32025 - 32035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ochotny, A. Van Vliet, N. Chan, Y. Yao, M. Morel, N. Kartner, H. P. von Schroeder, J. N. M. Heersche, and M. F. Manolson Effects of Human a3 and a4 Mutations That Result in Osteopetrosis and Distal Renal Tubular Acidosis on Yeast V-ATPase Expression and Activity J. Biol. Chem., September 8, 2006; 281(36): 26102 - 26111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Kane The Where, When, and How of Organelle Acidification by the Yeast Vacuolar H+-ATPase Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 177 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Malkus, L. A. Graham, T. H. Stevens, and R. Schekman Role of Vma21p in Assembly and Transport of the Yeast Vacuolar ATPase Mol. Biol. Cell, November 1, 2004; 15(11): 5075 - 5091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. D. Epple, I. Suriapranata, E.-L. Eskelinen, and M. Thumm Aut5/Cvt17p, a Putative Lipase Essential for Disintegration of Autophagic Bodies inside the Vacuole J. Bacteriol., October 15, 2001; 183(20): 5942 - 5955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cohen, N. Perzov, H. Nelson, and N. Nelson A Novel Family of Yeast Chaperons Involved in the Distribution of V-ATPase and Other Membrane Proteins J. Biol. Chem., September 17, 1999; 274(38): 26885 - 26893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Kane, M. Tarsio, and J. Liu Early Steps in Assembly of the Yeast Vacuolar H+-ATPase J. Biol. Chem., June 11, 1999; 274(24): 17275 - 17283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nelson and W. R. Harvey Vacuolar and Plasma Membrane Proton-Adenosinetriphosphatases Physiol Rev, April 1, 1999; 79(2): 361 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Frey and S. K. Randall Initial Steps in the Assembly of the Vacuole-Type H+-ATPase Plant Physiology, September 1, 1998; 118(1): 137 - 147. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. A. Graham, K. J. Hill, and T. H. Stevens Assembly of the Yeast Vacuolar H+-ATPase Occurs in the Endoplasmic Reticulum and Requires a Vma12p/Vma22p Assembly Complex J. Cell Biol., July 13, 1998; 142(1): 39 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. E. Oluwatosin and P. M. Kane Mutations in the Yeast KEX2 Gene Cause a Vma--Like Phenotype: a Possible Role for the Kex2 Endoprotease in Vacuolar Acidification Mol. Cell. Biol., March 1, 1998; 18(3): 1534 - 1543. [Abstract] [Full Text]
|
|
|
|
 |
|
 X. Li, R. T. C. Su, H.-t. Hsu, and H. Sze The Molecular Chaperone Calnexin Associates with the Vacuolar H+-ATPase from Oat Seedlings PLANT CELL, January 1, 1998; 10(1): 119 - 130. [Abstract] [Full Text]
|
|
|
|
|
|
Y. E. Oluwatosin and P. M. Kane Mutations in the CYS4 Gene Provide Evidence for Regulation of the Yeast Vacuolar H+-ATPase by Oxidation and Reduction in Vivo J. Biol. Chem., October 31, 1997; 272(44): 28149 - 28157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Jackson and T. H. Stevens VMA12 Encodes a Yeast Endoplasmic Reticulum Protein Required for Vacuolar H+-ATPase Assembly J. Biol. Chem., October 10, 1997; 272(41): 25928 - 25934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hirata, L. A. Graham, A. Takatsuki, T. H. Stevens, and Y. Anraku VMA11 and VMA16 Encode Second and Third Proteolipid Subunits of the Saccharomyces cerevisiae Vacuolar Membrane H+-ATPase J. Biol. Chem., February 21, 1997; 272(8): 4795 - 4803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tomashek, J. L. Sonnenburg, J. M. Artimovich, and D. J. Klionsky Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., April 26, 1996; 271(17): 10397 - 10404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Hill and T. H. Stevens Vma22p Is a Novel Endoplasmic Reticulum-associated Protein Required for Assembly of the Yeast Vacuolar H[IMAGE]-ATPase Complex J. Biol. Chem., September 22, 1995; 270(38): 22329 - 22336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Graham, K. J. Hill, and T. H. Stevens VMA8 Encodes a 32-kDa V[IMAGE] Subunit of the Saccharomyces cerevisiae Vacuolar H[IMAGE]-ATPase Required for Function and Assembly of the Enzyme Complex J. Biol. Chem., June 23, 1995; 270(25): 15037 - 15044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
u. Supeková, F.;e. Supek, and N. Nelson The SaccharomycescerevisiaeVMA10 Is an Intron-containing Gene Encoding a Novel 13-kDa Subunit of Vacuolar H[IMAGE]-ATPase J. Biol. Chem., June 9, 1995; 270(23): 13726 - 13732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Na, M. Hincapie, J. H. McCusker, and J. E. Haber MOP2 (SLA2) Affects the Abundance of the Plasma Membrane H[IMAGE]-ATPase of Saccharomyces cerevisiae J. Biol. Chem., March 24, 1995; 270(12): 6815 - 6823. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
